pIIIa (AC_000019) Virus Tagged ORF Clone

CAT#: VC100081

Myc-DDK-tagged ORF clone of viral ORF for pIIIa [Human adenovirus 35], codon optimized for human cell expression, AP_000579

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: pIIIa
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100081 represents NCBI reference of AP_000579 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACAGCAAGCTCCTGATCCGGCTATGCGCGCCGCATTGCAGAGCCAGCCAAGCGGAATAAACTCAT
  CCGACGATTGGACCCAGGCCATGCAGAGGATCATGGCCCTGACAACGAGAAACCCGGAAGCCTTCCGGCA
  GCAACCTCAGGCTAATCGCCTGTCCGCGATCATGGAAGCCGTGGTGCCTAGCCGGTCTAACCCAACACAC
  GAGAAGGTGTTGGCTATTGTGAATGCACTGGTAGAGAATAAAGCAATCAGGCCAGACGAGGCCGGGCTCG
  TGTATAACGCTTTGCTCGAGCGGGTGGCGAGATATAACAGTTCCAATGTCCAGACAAATTTGGACCGGAT
  GATCACGGATGTGCGGGAAGCAGTATCTCAGCGCGAACGCTTCCAGAGGGACGCGAATCTCGGATCTCTG
  GTGGCCCTCAACGCTTTTCTGAGCACACAGCCTGCTAACGTCCCACGGGGACAGCAGGATTACACAAACT
  TCCTCAGCGCCCTCCGCCTGATGGTGAGTGAGGTTCCTCAATCCGAAGTTTACCAGTCCGGACCCGACTA
  TTTCTTCCAAACCAGCCGGCAAGGACTCCAGACCGTTAATCTTAGCCAAGCTTTCAAAAATCTTAAGGGC
  CTGTGGGGGGTTCATGCCCCTGTGGGGGAGAGGGCAACTGTATCTTCCCTGCTGACTCCAAACTCTCGGC
  TGCTTCTCCTGCTGGTGGCCCCCTTTACCGATTCCGGCTCTATAGATAGGAATAGTTACCTCGGATATCT
  GCTGAATTTGTACCGCGAGGCTATCGGACAAAGCCAGGTGGATGAGCAGACCTACCAAGAGATAACTCAG
  GTGTCCCGGGCTTTGGGGCAGGAAGATACTGGCTCACTTGAGGCAACGTTGAATTTCCTGCTGACGAATA
  GGTCTCAGAAAATACCGCCCCAATACGCTCTGACCGCCGAAGAGGAGCGAATCCTGAGGTATGTCCAGCA
  GAGCGTGGGACTGTTTCTGATGCAGGAAGGCGCCACTCCCACTGCCGCCCTGGATATGACAGCTCGAAAC
  ATGGAACCCTCAATGTACGCCTCAAACCGCCCATTCATTAACAAACTGCTGGATTACCTCCATAGGGCGG
  CTGCAATGAACAGTGATTACTTTACCAACGCGATTTTGAACCCACACTGGTTGCCACCGCCAGGGTTTTA
  TACAGGCGAGTACGACATGCCTGATCCGAATGATGGCTTCCTTTGGGACGACGTGGACAGCGATGTATTC
  AGTCCCCTGTCCGATCATCGAACCTGGAAGAAAGAGGGGGGCGACCGGATGCATAGCTCTGCTTCCCTGT
  CAGGTGTTATGGGTGCTACCGCTGCTGAGCCGGAGAGTGCCTCACCCTTCCCTTCACTTCCATTTAGCCT
  TCACTCTGTGCGCAGCTCAGAGGTAGGCCGCATCAGTCGCCCCAGCCTGATGGGGGAGGAAGAGTATCTG
  AACGATTCTCTGCTCCGCCCAGCCAGAGAGAAGAATTTCCCAAATAACGGCATAGAATCTCTGGTGGATA
  AGATGTCCCGCTGGAAGACGTATGCCCAAGATCATAGAGATGAGCCAGGGATTATGGGTACCACATCTCG
  GGCGTCACGGCGGAGGCAACGCCACGATCGCCAGCGAGGCCTCGTATGGGATGATGAGGATAGCGCTGAC
  GACTCCAGCGTTCTTGATCTGGGCGGCAGAGGAAGAGGCAACCCTTTCGCTCACTTGCGCCCGAGACTGG
  GCGGTATGCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100081 representing AP_000579
  Red=Cloning sites Green=Tags
  
  MEQQAPDPAMRAALQSQPSGINSSDDWTQAMQRIMALTTRNPEAFRQQPQANRLSAIMEAVVPSRSNPTH
  EKVLAIVNALVENKAIRPDEAGLVYNALLERVARYNSSNVQTNLDRMITDVREAVSQRERFQRDANLGSL
  VALNAFLSTQPANVPRGQQDYTNFLSALRLMVSEVPQSEVYQSGPDYFFQTSRQGLQTVNLSQAFKNLKG
  LWGVHAPVGERATVSSLLTPNSRLLLLLVAPFTDSGSIDRNSYLGYLLNLYREAIGQSQVDEQTYQEITQ
  VSRALGQEDTGSLEATLNFLLTNRSQKIPPQYALTAEEERILRYVQQSVGLFLMQEGATPTAALDMTARN
  MEPSMYASNRPFINKLLDYLHRAAAMNSDYFTNAILNPHWLPPPGFYTGEYDMPDPNDGFLWDDVDSDVF
  SPLSDHRTWKKEGGDRMHSSASLSGVMGATAAEPESASPFPSLPFSLHSVRSSEVGRISRPSLMGEEEYL
  NDSLLRPAREKNFPNNGIESLVDKMSRWKTYAQDHRDEPGIMGTTSRASRRRQRHDRQRGLVWDDEDSAD
  DSSVLDLGGRGRGNPFAHLRPRLGGML
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000019
• ORF Size: 1761 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: AC_000019.1, AP_000579
• RefSeq ORF: 1761 bp
• MW: 65.6 kDa