OTOG (NM_001292063) Human Untagged Clone

CAT#: SC338155

OTOG (untagged) - Human otogelin (OTOG), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: OTOG
• Synonyms: DFNB18B; MLEMP; OTGN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001292063, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGAGTCCTGGCGTCTGCGCTCTGCTGGCTGCTTTGTGTCTGGCTGCCCTGGGGTGAGCAGGCAGCCG
  AGTCCCTGCGGGTGCAGCGCCTCGCCGCAGCACCCGTTCTGTGGGGCAGTGCAGAGCCACAGCCAGAGCC
  AGCCGGGCAACCCAGCAGCAGCCACCAGGAGGCGACCCTTGCCATGGGGGACAAGGCTACAGTCGTGGGA
  GGCCAGCAGGCTGAAGCCCCAGACTCCGTGGCCATGTCTTCCTGGGAAAGGCGGCTCCATCGGGCCAAGT
  GTGCACCATCCTACTTGTTCTCCTGCTTCAATGGAGGCGAGTGTGTGCACCCAGCCTTCTGTGACTGCAG
  ACGCTTCAATGCCACTGGACCGCGCTGCCAGATGGTGTACAATGCCGGCCCTGAGAGGGACAGCATTTGC
  CGGGCGTGGGGGCAGCACCACGTGGAGACATTTGATGGGCTCTACTACTACCTCTCCGGAAAGGGCAGCT
  ACACCCTGGTGGGTCGCCATGAGCCCGAGGGACAGAGCTTCTCCATCCAGGTACACAATGACCCGCAGTG
  TGGCTCTTCACCCTACACCTGCTCCAGGGCTGTCAGCCTCTTCTTTGTGGGTGAGCAGGAGATCCATCTG
  GCCAAGGAGGTCACCCATGGAGGCATGAGGGTCCAACTGCCACATGTCATGGGGAGCGCGCGTCTGCAGC
  AGCTTGCCGGCTATGTCATCGTGCGGCATCAGTCAGCCTTCACACTGGCCTGGGATGGTGCCTCGGCTGT
  CTACATCAAGATGAGTCCAGAGCTTCTGGGCTGGACCCATGGGCTGTGTGGGAACAACAATGCTGACCCC
  AAGGATGATCTGGTGACCAGCTCTGGGAAGCTGACTGACGACGTGGTTGAGTTTGTGCACAGCTGGCAGG
  AGCAGGCCCCTAACCAGCCTCCAGGGCCCACAACTTCCTCCCTGCCTCGCCCACCGTGCCTACAGCAGAA
  CCCAGGAACCATGCAGGGCGTGTACGAGCAGTGTGAGGCTCTACTGCGGCCCCCCTTTGACGCCTGCCAC
  GCCTACGTCAGCCCTCTGCCCTTCACAGCCAGTTGTACCAGTGATCTCTGCCAATCAATGGGTGATGTAG
  CCACCTGGTGCCGGGCACTGGCGGAGTATGCCCGGGCGTGTGCCCAGGCAGGGCGGCCCTTGCAAGGCTG
  GAGGACCCAGCTCCGGCAATGCACTGTGCACTGCAAGGAGAAGGCCTTTACCTACAATGAGTGCATCGCC
  TGCTGCCCTGCCTCCTGCCATCCCCGGGCATCCTGTGTGGACAGTGAGATCGCCTGTGTGGACGGCTGCT
  ATTGCCCCAATGGGCTCATCTTCGAGGATGGGGGCTGCGTGGCACCAGCTGAGTGTCCCTGTGAGTTTCA
  CGGGACTCTGTACCCACCTGGCTCTGTGGTGAAGGAAGACTGCAATACTTGCACATGCACCTCAGGCAAG
  TGGGAGTGCAGCACAGCTGTCTGCCCAGCTGAGTGCTCAGTGACTGGTGACATTCACTTCACAACCTTTG
  ATGGCCGCCGGTACACGTTCCCCGCCACATGTCAGTACATCCTGGCCAAGAGCCGCTCTTCGGGCACCTT
  CACCGTGACATTGCAGAATGCCCCATGTGGCCTGAACCAAGATGGAGCCTGTGTCCAGTCAGTGTCAGTG
  ATTCTGCACCAGGACCCTCGGAGGCAGGTGACCCTGACCCAGGCAGGGGATGTCCTTCTGTTTGACCAGT
  ACAAGATCATCCCGCCATACACAGATGATGCCTTTGAGATCCGTAGGCTGTCCTCCGTGTTCCTGCGGGT
  GAGGACGAACGTGGGCGTGCGGGTGCTCTACGACCGTGAAGGGCTCCGACTGTACCTGCAAGTGGACCAG
  CGATGGGTGGAGGATACCGTGGGCCTCTGCGGCACCTTCAATGGCAACACGCAGGATGACTTCCTGTCTC
  CAGTGGGTGTACCTGAGAGCACCCCACAACTTTTTGGCAATTCCTGGAAAACACTTTCTGCTTGCTCCCC
  GCTGGTCTCTGGCTCCCCTCTGGACCCCTGCGATGTGCACCTGCAAGCCGCCTCCTACTCAGTGCAGGCC
  TGCAGCGTGCTCACGGGGGAGATGTTTGCGCCCTGCTCTGCGTTCCTGAGCCCCGTGCCCTACTTTGAGC
  AGTGCCGCAGGGATGCCTGCCGCTGCGGGCAGCCCTGCCTGTGCGCCACACTGGCCCACTACGCCCACCT
  GTGCCGGCGCCATGGGCTCCCCGTTGATTTCCGCGCCCGCCTGCCAGCCTGTGCACTGTCCTGTGAGGCC
  TCCAAGGAGTATAGCCCCTGCGTGGCCCCGTGTGGACGTACCTGCCAGGACCTGGCCAGCCCTGAGGCCT
  GTGGGGTTGATGGTGGCGATGACCTGAGCAGAGACGAGTGTGTGGAGGGCTGTGCCTGCCCACCGGACAC
  CTATCTGGACACCCAGGCTGACCTCTGTGTCCCCCGGAACCAGTGCTCCTGCCACTTCCAGGGAGTGGAC
  TATCCCCCCGGAGACAGTGACATCCCATCCCTGGGCCACTGCCACTGCAAAGATGGAGTCATGAGCTGTG
  ATAGCAGAGCCCCAGCTGCTGCCTGCCCAGCAGGCCAGGTCTTCGTGAACTGCAGCGACCTGCACACGGA
  CCTGGAGCTGAGCAGGGAGAGGACGTGTGAGCAGCAACTGCTGAACCTGAGCGTGTCAGCCCGTGGCCCC
  TGCCTCTCGGGCTGCGCCTGTCCCCAGGGTCTGCTCAGACACGGGGATGCATGTTTCCTGCCAGAGGAGT
  GCCCCTGCACTTGGAAGGGGAAGGAGTATTTCCCTGGGGACCAGGTGATGTCTCCTTGCCATACCTGTGT
  GTGCCAGCGGGGCTCATTCCAGTGCACCCTGCACCCTTGCGCCTCCACCTGCACTGCCTATGGGGACCGG
  CATTACCGCACGTTTGATGGGCTCCCGTTTGACTTCGTGGGGGCATGCAAAGTGCACCTGGTCAAGAGCA
  CATCAGATGTCAGCTTCTCTGTGATTGTAGAGAATGTGAACTGCTACAGCTCTGGCATGATCTGCAGGAA
  ATTTATTTCCATCAACGTTGGGAACTCACTCATTGTCTTTGATGATGACTCCGGAAATCCTAGTCCAGAG
  AGCTTCCTGGATGACAAGCAGGAGGTCCACACATGGCGAGTGGGATTTTTCACACTGGTGCATTTCCCAC
  AGGAGCACATCACCCTCTTGTGGGACCAGAGAACCACAGTGCACGTCCAGGCTGGGCCTCAGTGGCAGGG
  CCAGCTGGCGGGCCTCTGTGGGAACTTTGACTTAAAAACCATCAATGAGATGAGGACCCCGGAGAACCTA
  GAGCTAACTAACCCCCAGGAGTTTGGCAGCAGTTGGGCTGCAGTTGAGTGCCCAGACACCCTCGATCCTC
  GGGATATGTGTGTCCTGAATCCTCTCCGAGAACCATTTGCCAAGAAGGAGTGCAGCATCCTGCTCAGTGA
  GGTGTTTGAGATCTGCCACCCTGTGGTTGATGTCACTTGGTTTTACTCAAACTGCCTGACAGACACATGT
  GGCTGCAGCCAGGGTGGTGACTGTGAGTGCTTCTGTGCCAGCGTCTCCGCTTATGCCCACCAGTGTTGCC
  AGCATGGGGTGGCTGTTGACTGGCGAACCCCCCGCCTCTGCCCGTATGACTGTGACTTCTTTAACAAAGT
  GCTAGGTAAGGGCCCCTATCAGCTATCCAGCTTGGCAGCCGGTGGTGCTCTGGTGGGCATGAAGGCGGTG
  GGCGATGACATAGTCCTAGTGAGGACAGAGGATGTGGCGCCAGCAGACATTGTGAGCTTCCTGCTGACAG
  CTGCTCTGTACAAGGCCAAGGCCCATGACCCAGATGTGGTGTCCCTGGAGGCAGCAGACAGACCCAACTT
  CTTCCTTCACGTCACAGCCAACGGGTCTCTGGAGCTGGCTAAGTGGCAGGGCCGTGACACCTTCCAACAG
  CATGCCTCCTTCTTGCTGCACCGGGGGACACGGCAGGCAGGCCTGGTGGCCCTGGAGTCCCTGGCCAAGC
  CCAGCTCCTTCCTCTATGTGTCGGGCGCGGTGCTGGCCCTGCGGCTGTACGAACACACAGAGGTGTTCCG
  CCGGGGCACACTCTTCCGCCTTCTGGATGCCAAGCCCTCGGGGGCTGCCTACCCCATCTGCGAGTGGCGC
  TACGATGCCTGTGCCAGCCCCTGCTTCCAAACCTGCCGGGACCCACGGGCAGCCAGCTGCCGGGACGTAC
  CCAGGGTAGAAGGCTGTGTCCCTGTGTGCCCCACCCCCCAGGTCCTGGATGAAGTCACACAGAGATGTGT
  CTACTTGGAGGACTGTGTGGAGCCAGCAGTTTGGGTTCCCACAGAGGCCCTTGGCAATGAGACCCTCCCT
  CCCAGTCAAGGGTTGCCCACTCCCAGTGATGAGGAGCCACAGCTGTCACAGGAAAGCCCCAGGACCCCCA
  CCCACAGGCCAGCCCTCACCCCAGCTGCCCCACTCACCACAGCCCTGAACCCACCAGTGACAGCCACTGA
  GGAGCCAGTGGTGTCTCCAGGCCCCACCCAGACCACCCTGCAGCAGCCACTGGAGCTCACTGCATCTCAA
  CTCCCCGCCGGCCCCACGGAGTCCCCAGCCAGCAAGGGAGTGACTGCCAGCCTCCTGGCCATCCCCCATA
  CACCAGAGTCCTCATCCCTCCCTGTTGCACTGCAGACACCCACACCTGGCATGGTGTCAGGTGCCATGGA
  GACAACAAGGGTGACTGTGATCTTTGCAGGAAGCCCTAACATCACAGTCTCCTCCCGGTCGCCCCCTGCC
  CCTCGCTTCCCGCTCATGACCAAGGCTGTGACAGTCCGAGGCCATGGCTCCTTGCCTGTTAGGACGACAC
  CCCCACAGCCCTCCTTGACAGCAAGTCCCTCCTCCAGACCTGTGGCTTCCCCTGGAGCCATCTCCAGGTC
  CCCCACCTCCTCGGGATCCCACAAGGCTGTGCTGACACCTGCAGTAACTAAGGTCATAAGCAGGACAGGG
  GTCCCCCAGCCCACCCAGGCCCAGAGTGCTTCAAGTCCCAGCACCCCTCTAACTGTGGCTGGAACAGCAG
  CAGAACAGGTTCCTGTCAGTCCCCTTGCAACCAGGAGCTTGGAGATAGTGCTATCCACAGAGAAGGGCGA
  AGCCGGGCACAGCCAGCCCATGGGCTCGCCTGCCTCCCCACAGCCACACCCACTCCCCTCTGCACCACCC
  CGCCCAGCCCAGCATACCACCATGGCCACCAGGTCTCCAGCTCTGCCCCCAGAGACCCCAGCTGCCGCCA
  GCCTGTCAACAGCCACTGATGGGCTGGCAGCCACACCCTTCATGTCCCTTGAGTCAACTCGTCCCTCCCA
  GCTCCTCTCTGGCCTGCCTCCCGACACCAGCCTGCCCCTGGCCAAGGTGGGCACATCTGCCCCAGTGGCC
  ACACCCGGCCCCAAAGCCTCTGTCATCACCACTCCACTCCAGCCACAGGCCACGACTCTGCCTGCTCAGA
  CACTTAGCCCAGTACTGCCTTTCACTCCAGCAGCAATGACCCAGGCGCACCCACCCACTCACATAGCACC
  CCCAGCAGCAGGCACAGCTCCAGGCCTGCTGCTGGGAGCCACATTGCCAACCTCTGGAGTCCTGCCTGTG
  GCTGAGGGCACGGCCTCCATGGTATCTGTTGTCCCACGAAAGAGCACCACAGGGAAGGTGGCCATCCTAT
  CCAAGCAAGTGTCTCTGCCCACTTCCATGTATGGTTCTGCAGAGGGTGGGCCCACAGAGCTCACGCCTGC
  TACGAGCCACCCTCTCACGCCCTTGGTGGCTGAGCCCGAGGGAGCCCAGGCAGGCACAGCTCTGCCAGTG
  CCCACATCCTATGCCCTGAGCCGTGTCTCAGCCAGGACGGCCCCCCAAGACAGCATGCTGGTTCTGTTGC
  CTCAGCTGGCTGAGGCCCATGGAACCTCGGCAGGGCCTCACCTGGCAGCAGAGCCGGTGGACGAGGCCAC
  CACAGAACCATCTGGGCGCTCAGCCCCAGCCCTGAGCATCGTAGAGGGTTTGGCGGAGGCTTTGGCAACT
  ACCACTGAGGCCAATACATCCACCACCTGTGTTCCAATCGCCGAGCAGGACTGCGTCCGCCACATCTGCC
  TGGAGGGCCAGCTGATTCGCGTGAATCAGTCCCAGCACTGTCCCCAGGGTGCTGCTCCCCCTCGCTGTGG
  GATCCTGGGCCTCGCCGTGCGGGTGGGTGGGGACCGCTGCTGCCCACTCTGGGAGTGTGCCTGCCGGTGC
  TCAATCTTCCCTGACCTGAGCTTCGTGACCTTCGATGGGAGCCACGTAGCTCTGTTCAAGGAGGCCATCT
  ACATCCTCAGCCAGAGCCCAGATGAAATGCTCACCGTCCATGTACTGGACTGCAAAAGTGCCAACCTGGG
  GCACCTGAACTGGCCCCCGTTCTGTCTGGTGATGTTGAACATGACTCACTTGGCCCATCAGGTCACTATT
  GATCGCTTCAACCGAAAGGTGACTGTGGACTTGCAGCCTGTGTGGCCACCGGTGAGCAGGTATGGATTCA
  GAATTGAGGACACAGGCCACATGTACATGATCCTGACTCCCTCAGACATCCAGATCCAGTGGCTCCACAG
  CTCAGGACTCATGATCGTGGAGGCCAGCAAAACCAGCAAGGCCCAGGGCCATGGCCTGTGCGGTATCTGT
  GATGGAGATGCAGCCAATGACCTTACCCTGAAGGATGGCTCAGTGGTGGGTGGGGCTGAGGACCCTGCTC
  CCTTTCTGGACAGCTGGCAGGTGCCCAGCTCCCTGACCTCAGTGGGCCAGACCCGCTTCCGCCCAGACAG
  CTGCGCCACAACTGACTGCTCGCCCTGCCTTCGCATGGTGTCCAACCGCACCTTCAGTGCCTGCCACCGC
  TTTGTGCCTCCGGAGTCATTCTGTGAGCTGTGGATCCGGGACACCAAGTACGTGCAGCAGCCCTGCGTGG
  CCCTGACTGTGTACGTGGCCATGTGCCACAAATTTCATGTGTGCATCGAGTGGCGGCGCTCTGACTACTG
  CCCCTTCCTGTGCTCCAGCGACTCCACATACCAGGCATGTGTGACAGCCTGTGAGCCACCCAAGACATGC
  CAGGATGGGATACTAGGGCCTCTGGACCCAGAGCACTGCCAGGTGCTGGGCGAGGGCTGCGTCTGCTCCG
  AGGGCACCATCTTACACCGGCGCCACTCTGCACTCTGCATCCCGGAGGCCAAGTGCGCCTGCACTGACAG
  CATGGGGGTGCCGAGGGCCCTGGGGGAGACCTGGAACAGCTCCCTCAGCGGCTGCTGCCAGCACCAGTGC
  CAAGCCCCAGACACCATTGTCCCGGTGGATCTGGGCTGCCCCAGTCCCCGCCCTGAGAGCTGCCTGCGAT
  TCGGGGAGGTGGCCTTGCTCCTACCCACCAAGGACCCCTGCTGCCTGGGGACTGTCTGTGTGTGTAACCA
  GACTCTGTGTGAGGGTCTCGCCCCCACATGCCGCCCAGGCCACCGCCTCCTCACCCACTTCCAGGAGGAC
  TCCTGCTGCCCCAGCTACAGCTGTGAGTGTGACCCAGATCTCTGTGAGGCAGAGCTGGTCCCCAGCTGCC
  GACAGGACCAGATCCTGATCACGGGCCGCCTGGGGGACTCCTGCTGCACCTCCTACTTCTGCGCCTGTGG
  TGACTGTCCAGACTCCATCCCCGAATGTCAAGAAGGGGAGGCGCTCACTGTGCACAGGAATACCACGGAA
  CTCTGCTGCCCTCTGTACCAGTGTGTGTGTGAGAACTTCCGCTGTCCCCAAGTGCAGTGTGGCCTGGGCA
  CTGCCCTGGTGGAGGTGTGGAGCCCCGACCGCTGCTGCCCCTACAAATCCTGTGAATGTGACTGTGACAC
  AATCCCGGTGCCCCGGTGCCATCTGTGGGAGAAATCCCAGCTGGATGAGGAGTTCATGCACAGCGTGGAG
  AATGTGTGTGGCTGCGCCAAGTACGAGTGTGTGAAGGCCCCGGTGTGTCTGAGCCGCGAGCTGGGTGTGA
  TGCAGCCCGGCCAGACAGTGGTGGAGCTCTCAGCAGATGGCGTGTGCCACACCTCCCGCTGCACCACCGT
  GCTCGACCCTCTCACCAACTTCTACCAGATCAACACCACCTCCGTGCTCTGTGACATCCACTGTGAGGCG
  AACCAGGAGTACGAGCACCCGCGGGACCTCGCTGCCTGCTGCGGCTCCTGCAGGAACGTGTCCTGTCTCT
  TCACCTTCCCCAATGGCACCACCTCCCTGTTCTTGCCCGGGGCATCCTGGATCGCAGACTGCGCCCGCCA
  CCACTGCAGCAGCACGCCCCTGGGTGCCGTGCTGGTCCGCTCTCCCATAAGCTGCCCACCGCTCAATGAG
  ACTGAGTGTGCCAAGGTTGGGGGTTCCGTGGTACCTTCCTTGGAAGGATGCTGCAGGACCTGTAAGGAGG
  ATGGGCGCTCCTGCAAGAAGGTGACCATCCGCATGACCATCCGCAAGAATGAATGCAGGAGCAGCACCCC
  TGTGAACCTAGTGTCCTGCGATGGGAGGTGCCCATCCGCCAGCATCTACAACTACAACATCAACACCTAT
  GCCCGATTCTGCAAGTGCTGCCGTGAGGTGGGCCTGCAGCGGCGCTCTGTGCAGCTCTTCTGTGCCACCA
  ATGCCACCTGGGTGCCCTATACAGTGCAGGAGCCCACCGACTGTGCCTGCCAGTGGTCCTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001292063
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001292063.1, NP_001278992.1
• RefSeq Size: 8742 bp
• RefSeq ORF: 8742 bp
• Locus ID: 340990
• Cytogenetics: 11p15.1
• Gene Summary: The protein encoded by this gene is a component of the acellular membranes of the inner ear. Disruption of the orthologous mouse gene shows that it plays a role in auditory and vestibular functions. It is involved in fibrillar network organization, the anchoring of otoconial membranes and cupulae to the neuroepithelia, and likely in sound stimulation resistance. Mutations in this gene cause autosomal recessive nonsyndromic deafness, type 18B. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2014]
  Transcript Variant: This variant (2) uses two alternate splice sites and includes an additional exon, which together result in an alternate in-frame segment in the 5' coding region, compared to variant 1. The encoded isoform (b) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The exon combination of this transcript is inferred from the full-length mouse mRNA U96411.1, and is supported by partial human transcript alignments, RNA-seq data, and partial RT-PCR data from PMID:23122587. CCDS Note: This CCDS representation lacks full-length human transcript support. Its exon combination is therefore inferred, but it is supported by the full-length orthologous mouse transcript U96411.1, by partial human transcript alignments, and by data in PMID:23122587. ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##