HECW1 (NM_001287059) Human Untagged Clone

CAT#: SC338073

HECW1 (untagged) - Human HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HECW1
• Synonyms: NEDL1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001287059, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCTGCTGCACCTGTGTAGTGTGAAGAATCTGTACCAGAACAGGTTTTTAGGCCTGGCCGCCATGGCGT
  CTCCTTCTAGAAACTCCCAGAGCCGACGCCGGTGCAAGGAGCCGCTCCGATACAGCTACAACCCCGACCA
  GTTCCACAACATGGACCTCAGGGGCGGCCCCCACGATGGCGTCACCATTCCCCGCTCCACCAGCGACACT
  GACCTGGTCACCTCGGACAGCCGCTCCACGCTCATGGTCAGCAGCTCCTACTATTCCATCGGGCACTCTC
  AGGACCTGGTCATCCACTGGGACATAAAGGAGGAAGTGGACGCTGGGGACTGGATTGGCATGTACCTCAT
  TGATGAGGTCTTGTCCGAAAACTTTCTGGACTATAAAAACCGTGGAGTCAATGGTTCTCATCGGGGCCAG
  ATCATCTGGAAGATCGATGCCAGCTCGTACTTTGTGGAACCTGAAACTAAGATCTGCTTCAAATACTACC
  ATGGAGTGAGTGGGGCCCTGCGAGCAACCACCCCCAGTGTCACGGTCAAAAACTCGGCAGCTCCTATTTT
  TAAAAGCATTGGTGCTGATGAGACCGTCCAAGGACAAGGAAGTCGGAGGCTGATCAGCTTCTCTCTCTCA
  GATTTCCAAGCCATGGGGTTGAAGAAAGGGATGTTTTTCAACCCAGACCCTTATCTGAAGATTTCCATTC
  AGCCTGGGAAACACAGCATCTTCCCCGCCCTCCCTCACCATGGACAGGAGAGGAGATCCAAGATCATAGG
  CAACACCGTGAACCCCATCTGGCAGGCCGAGCAATTCAGTTTTGTGTCCTTGCCCACTGACGTGCTGGAA
  ATTGAGGTGAAGGACAAGTTTGCCAAGAGCCGCCCCATCATCAAGCGCTTCTTGGGAAAGCTGTCGATGC
  CCGTTCAAAGACTCCTGGAGAGACACGCCATAGGGGATAGGGTGGTCAGCTACACACTTGGCCGCAGGCT
  TCCAACAGATCATGTGAGTGGACAGCTGCAATTCCGATTTGAGATCACTTCCTCCATCCACCCAGATGAT
  GAGGAGATTTCCCTGAGTACCGAGCCTGAGTCAGCCCAAATTCAGGACAGCCCCATGAACAACCTGATGG
  AAAGCGGCAGTGGGGAACCTCGGTCTGAGGCACCAGAGTCCTCTGAGAGCTGGAAGCCAGAGCAGCTGGG
  TGAGGGCAGTGTCCCCGATGGTCCAGGGAACCAAAGCATAGAGCTTTCCAGACCAGCTGAGGAAGCAGCA
  GTCATCACGGAGGCAGGAGACCAGGGCATGGTCTCTGTGGGACCTGAAGGGGCTGGGGAGCTCCTGGCCC
  AGGTGCAAAAGGACATCCAGCCTGCCCCCAGTGCAGAAGAGCTGGCCGAGCAGCTGGACCTGGGTGAGGA
  GGCATCAGCACTGCTGCTGGAAGACGGTGAAGCCCCAGCCAGCACCAAGGAGGAGCCCTTGGAGGAGGAA
  GCAACGACCCAGAGCCGGGCTGGAAGGGAAGAAGAGGAGAAGGAGCAGGAGGAGGAGGGAGATGTGTCTA
  CCCTGGAGCAGGGAGAGGGCAGGCTGCAGCTGCGGGCCTCGGTGAAGAGAAAAAGCAGGCCCTGCTCCTT
  GCCTGTGTCCGAGCTGGAGACGGTGATCGCGTCAGCCTGCGGGGACCCCGAGACCCCGCGGACACACTAC
  ATCCGCATCCACACCCTGCTGCACAGCATGCCCTCCGCCCAGGGCGGCAGCGCGGCAGAGGAGGAGGACG
  GCGCGGAGGAGGAGTCCACCCTCAAGGACTCCTCGGAGAAGGATGGGCTCAGCGAGGTGGACACGGTGGC
  CGCTGACCCGTCTGCCCTGGAAGAGGACAGAGAAGAGCCCGAGGGGGCTACTCCAGGCACGGCGCACCCT
  GGCCACTCCGGGGGCCACTTCCCCAGCCTGGCCAATGGCGCGGCCCAGGATGGCGACACGCACCCCAGCA
  CCGGGAGCGAGAGCGACTCCAGCCCCAGGCAAGGCGGGGACCACAGTTGCGAGGGCTGTGACGCGTCCTG
  CTGCAGCCCCTCGTGCTACAGCTCCTCGTGCTACAGCACGTCCTGCTACAGCAGCTCGTGCTACAGCGCC
  TCGTGCTACAGCCCCTCCTGCTACAACGGCAACAGGTTCGCCAGCCACACGCGCTTCTCCTCCGTGGACA
  GCGCCAAGATCTCCGAGAGCACGGTCTTCTCCTCGCAAGACGACGAGGAGGAGGAGAACAGCGCGTTCGA
  GTCGGTACCCGACTCCATGCAGAGCCCTGAGCTGGACCCGGAGTCCACGAACGGCGCTGGGCCGTGGCAA
  GACGAGCTGGCCGCCCCTAGCGGGCACGTGGAAAGAAGCCCGGAAGGTCTGGAATCCCCCGTGGCAGGTC
  CAAGCAATCGGAGAGAAGACTGGGAAGCTCGAATTGACAGCCACGGGCGGGTCTTTTATGTGGACCACGT
  GAACCGCACAACCACCTGGCAGCGTCCGACGGCAGCAGCCACCCCGGATGGCATGCGGAGATCGGGGTCC
  ATCCAGCAGATGGAGCAACTCAACAGGCGGTATCAAAACATTCAGCGAACCATTGCAACAGAGAGGTCCG
  AAGAAGATTCTGGCAGCCAAAGCTGCGAGCAAGCCCCAGCAGGAGGAGGCGGAGGTGGAGGGAGTGACTC
  AGAAGCCGAATCTTCCCAGTCCAGCTTAGATCTAAGGAGAGAAGGGTCACTTTCTCCAGTGAACTCACAA
  AAAATCACCTTGCTGCTGCAGTCCCCAGCGGTCAAGTTCATCACCAACCCCGAGTTCTTCACTGTGCTAC
  ATGCCAATTATAGTGCCTACCGAGTCTTCACCAGTAGCACCTGCTTAAAGCACATGATTCTGAAAGTCCG
  ACGGGATGCTCGCAATTTTGAACGCTACCAGCACAACCGGGACTTGGTGAATTTCATCAACATGTTCGCA
  GACACTCGGCTGGAACTGCCCCGGGGCTGGGAGATCAAAACGGACCAGCAGGGAAAGTCTTTTTTCGTGG
  ACCACAACAGTCGAGCTACCACTTTCATTGACCCCCGAATCCCTCTTCAGAACGGTCGTCTTCCCAATCA
  TCTAACTCACCGACAGCACCTCCAGAGGCTCCGAAGTTACAGCGCTGGAGAGGCCTCAGAAGTTTCTAGA
  AACAGAGGAGCCTCTTTACTGGCCAGGCCAGGACACAGCTTAGTAGCTGCTATTCGAAGCCAACATCAAC
  ATGAGTCATTGCCACTGGCATATAATGACAAGATTGTGGCATTTCTTCGCCAGCCAAACATTTTTGAAAT
  GCTGCAAGAGCGTCAGCCAAGCTTAGCAAGAAACCACACACTCAGGGAGAAAATCCATTACATTCGGACT
  GAGGGTAATCACGGGCTTGAGAAGTTGTCCTGTGATGCGGATCTGGTCATTTTGCTGAGTCTCTTTGAAG
  AAGAGATTATGTCCTACGTCCCCCTGCAGGCTGCCTTCCACCCTGGGTATAGCTTCTCTCCCCGATGTTC
  ACCCTGTTCTTCACCTCAGAACTCCCCAGGTTTACAGAGAGCCAGTGCAAGAGCCCCTTCCCCCTACCGA
  AGAGACTTTGAGGCCAAGCTCCGCAATTTCTACAGAAAACTGGAAGCCAAAGGATTTGGTCAGGGTCCGG
  GGAAAATTAAGCTCATTATTCGCCGGGATCATTTGTTGGAGGGAACCTTCAATCAGGTGATGGCCTATTC
  GCGGAAAGAGCTCCAGCGAAACAAGCTCTACGTCACCTTTGTTGGAGAGGAGGGCCTGGACTACAGTGGC
  CCCTCGCGGGAGTTCTTCTTCCTTCTGTCTCAGGAGCTCTTCAACCCTTACTATGGACTCTTTGAGTACT
  CGGCAAATGATACTTACACGGTGCAGATCAGCCCCATGTCCGCATTTGTAGAAAACCATCTTGAGTGGTT
  CAGGTTTAGCGGTCGCATCCTGGGTCTGGCTCTGATCCATCAGTACCTTCTTGACGCTTTCTTCACGAGG
  CCCTTCTACAAGGCACTCCTGAGACTGCCCTGTGATTTGAGTGACCTGGAATATTTGGATGAGGAATTCC
  ACCAGAGTTTGCAGTGGATGAAGGACAACAACATCACAGACATCTTAGACCTCACTTTCACTGTTAATGA
  AGAGGTTTTTGGACAGGTCACGGAAAGGGAGTTGAAGTCTGGAGGAGCCAACACACAGGTGACGGAGAAA
  AACAAGAAGGAGTACATCGAGCGCATGGTGAAGTGGCGGGTGGAGCGCGGCGTGGTACAGCAGACCGAGG
  CGCTGGTGCGCGGCTTCTACGAGGTTGTAGACTCGAGGCTGGTGTCCGTGTTTGATGCCAGGGAGCTGGA
  GCTGGTGATAGCTGGCACCGCGGAAATCGACCTAAATGACTGGCGGAATAACACTGAGTACCGGGGAGGT
  TACCACGATGGGCATCTTGTGATCCGCTGGTTCTGGGCTGCGGTGGAGCGCTTCAATAATGAGCAGAGGC
  TGAGATTACTGCAGTTTGTCACGGGAACATCCAGCGTGCCCTACGAAGGCTTCGCAGCCCTCCGTGGGAG
  CAATGGGCTTCGGCGCTTCTGCATAGAGAAATGGGGGAAAATTACTTCTCTCCCCAGGGCACACACATGC
  TTCAACCGACTGGATCTTCCACCGTATCCCTCGTACTCCATGTTGTATGAAAAGCTGTTAACAGCAGTAG
  AGGAAACCAGCACCTTTGGACTTGAGTGA
  
  
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001287059
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001287059.1, NP_001273988.1
• RefSeq Size: 6523 bp
• RefSeq ORF: 4719 bp
• Locus ID: 23072
• UniProt ID: Q76N89
• Cytogenetics: 7p14.1-p13
• Gene Summary: E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent degradation of DVL1. Also targets the mutant SOD1 protein involved in familial amyotrophic lateral sclerosis (FALS). Forms cytotoxic aggregates with DVL1, SSR3 and mutant SOD1 that lead to motor neuron death in FALS.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR and lacks an alternate in-frame exon in the central coding region, compared to variant 1, resulting in an isoform (b) that is shorter than isoform a.