Lubricin (PRG4) (NM_001303232) Human Untagged Clone
CAT#: SC338019
PRG4 (untagged) - Human proteoglycan 4 (PRG4), transcript variant E
"NM_001303232" in other vectors (2)
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Frequently bought together (4)
Other products for "PRG4"
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Symbol | PRG4 |
Synonyms | CACP; HAPO; JCAP; MSF; SZP |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>NCBI ORF sequence for NM_001303232, the custom clone sequence may differ by one or more nucleotides
ATGGCATGGAAAACACTTCCCATTTACCTGTTGTTGCTGCTGTCTGTTTTCGTGATTCAGCAAGTTTCAT CTCAAGATTTATCAAGCTGTGCAGGGAGATGTGGGGAAGGGTATTCTAGAGATGCCACCTGCAACTGTGA TTATAACTGTCAACACTACATGGAGTGCTGCCCTGATTTCAAGAGAGTCTGCACTGCGGAGCTTTCCTGT AAAGGCCGCTGCTTTGAGTCCTTCGAGAGAGGGAGGGAGTGTGACTGCGACGCCCAATGTAAGAAGTATG ACAAGTGCTGTCCCGATTATGAGAGTTTCTGTGCAGAAGTGCATAATCCCACATCACCACCATCTTCAAA GAAAGCACCTCCACCTTCAGGAGCATCTCAAACCATCAAATCAACAACCAAACGTTCACCCAAACCACCA AACAAGAAGAAGACTAAGAAAGTTATAGAATCAGAGGAAATAACAGAAGTAAAAGATAACAAGAAGAACA GAACTAAAAAGAAACCTACCCCCAAACCACCAGTTGTAGATGAAGCTGGAAGTGGATTGGACAATGGTGA CTTCAAGGTCACAACTCCTGACACGTCTACCACCCAACACAATAAAGTCAGCACATCTCCCAAGATCACA ACAGCAAAACCAATAAATCCCAGACCCAGTCTTCCACCTAATTCTGATACATCTAAAGAGACGTCTTTGA CAGTGAATAAAGAGACAACAGTTGAAACTAAAGAAACTACTACAACAAATAAACAGACTTCAACTGATGG AAAAGAGAAGACTACTTCCGCTAAAGAGACACAAAGTATAGAGAAAACATCTGCTAAAGATTTAGCACCC ACATCTAAAGTGCTGGCTAAACCTACACCCAAAGCTGAAACTACAACCAAAGGCCCTGCTCTCACCACTC CCAAGGAGCCCACGCCCACCACTCCCAAGGAGCCTGCATCTACCACACCCAAAGAGCCCACACCTACCAC CATCAAGTCTGCACCCACCACCCCCAAGGAGCCTGCACCCACCACCACCAAGTCTGCACCCACCACTCCC AAGGAGCCTGCACCCACCACCACCAAGGAGCCTGCACCCACCACTCCCAAGGAGCCTGCACCCACCACCA CCAAGGAGCCTGCACCCACCACCACCAAGTCTGCACCCACCACTCCCAAGGAGCCTGCACCCACCACCCC CAAGAAGCCTGCCCCAACTACCCCCAAGGAGCCTGCACCCACCACTCCCAAGGAGCCTACACCCACCACT CCCAAGGAGCCTGCACCCACCACCAAGGAGCCTGCACCCACCACTCCCAAAGAGCCTGCACCCACTGCCC CCAAGAAGCCTGCCCCAACTACCCCCAAGGAGCCTGCACCCACCACTCCCAAGGAGCCTGCACCCACCAC CACCAAGGAGCCTTCACCCACCACTCCCAAGGAGCCTGCACCCACCACCACCAAGTCTGCACCCACCACT ACCAAGGAGCCTGCACCCACCACTACCAAGTCTGCACCCACCACTCCCAAGGAGCCTTCACCCACCACCA CCAAGGAGCCTGCACCCACCACTCCCAAGGAGCCTGCACCCACCACCCCCAAGAAGCCTGCCCCAACTAC CCCCAAGGAGCCTGCACCCACCACTCCCAAGGAACCTGCACCCACCACCACCAAGAAGCCTGCACCCACC ACTCCCAAAGAGCCTGCCCCAACTACCCCCAAGGAGACTGCACCCACCACCCCCAAGAAGCTCACGCCCA CCACCCCCGAGAAGCTCGCACCCACCACCCCTGAGAAGCCCGCACCCACCACCCCTGAGGAGCTCGCACC CACCACCCCTGAGGAGCCCACACCCACCACCCCTGAGGAGCCTGCTCCCACCACTCCCAAGGCAGCGGCT CCCAACACCCCTAAGGAGCCTGCTCCAACTACCCCTAAGGAGCCTGCTCCAACTACCCCTAAGGAGCCTG CTCCAACTACCCCTAAGGAGACTGCTCCAACTACCCCTAAAGGGACTGCTCCAACTACCCTCAAGGAACC TGCACCCACTACTCCCAAGAAGCCTGCCCCCAAGGAGCTTGCACCCACCACCACCAAGGAGCCCACATCC ACCACCTCTGACAAGCCCGCTCCAACTACCCCTAAGGGGACTGCTCCAACTACCCCTAAGGAGCCTGCTC CAACTACCCCTAAGGAGCCTGCTCCAACTACCCCTAAGGGGACTGCTCCAACTACCCTCAAGGAACCTGC ACCCACTACTCCCAAGAAGCCTGCCCCCAAGGAGCTTGCACCCACCACCACCAAGGGGCCCACATCCACC ACCTCTGACAAGCCTGCTCCAACTACACCTAAGGAGACTGCTCCAACTACCCCCAAGGAGCCTGCACCCA CTACCCCCAAGAAGCCTGCTCCAACTACTCCTGAGACACCTCCTCCAACCACTTCAGAGGTCTCTACTCC AACTACCACCAAGGAGCCTACCACTATCCACAAAAGCCCTGATGAATCAACTCCTGAGCTTTCTGCAGAA CCCACACCAAAAGCTCTTGAAAACAGTCCCAAGGAACCTGGTGTACCTACAACTAAGACTCCTGCAGCGA CTAAACCTGAAATGACTACAACAGCTAAAGACAAGACAACAGAAAGAGACTTACGTACTACACCTGAAAC TACAACTGCTGCACCTAAGATGACAAAAGAGACAGCAACTACAACAGAAAAAACTACCGAATCCAAAATA ACAGCTACAACCACACAAGTAACATCTACCACAACTCAAGATACCACACCATTCAAAATTACTACTCTTA AAACAACTACTCTTGCACCCAAAGTAACTACAACAAAAAAGACAATTACTACCACTGAGATTATGAACAA ACCTGAAGAAACAGCTAAACCAAAAGACAGAGCTACTAATTCTAAAGCGACAACTCCTAAACCTCAAAAG CCAACCAAAGCACCCAAAAAACCCACTTCTACCAAAAAGCCAAAAACAATGCCTAGAGTGAGAAAACCAA AGACGACACCAACTCCCCGCAAGATGACATCAACAATGCCAGAATTGAACCCTACCTCAAGAATAGCAGA AGCCATGCTCCAAACCACCACCAGACCTAACCAAACTCCAAACTCCAAACTAGTTGAAGTAAATCCAAAG AGTGAAGATGCAGGTGGTGCTGAAGGAGAAACACCTCATATGCTTCTCAGGCCCCATGTGTTCATGCCTG AAGTTACTCCCGACATGGATTACTTACCGAGAGTACCCAATCAAGGCATTATCATCAATCCCATGCTTTC CGATGAGACCAATATATGCAATGGTAAGCCAGTAGATGGACTGACTACTTTGCGCAATGGGACATTAGTT GCATTCCGAGGTCATTATTTCTGGATGCTAAGTCCATTCAGTCCACCATCTCCAGCTCGCAGAATTACTG AAGTTTGGGGTATTCCTTCCCCCATTGATACTGTTTTTACTAGGTGCAACTGTGAAGGAAAAACTTTCTT CTTTAAGGATTCTCAGTACTGGCGTTTTACCAATGATATAAAAGATGCAGGGTACCCCAAACCAATTTTC AAAGGATTTGGAGGACTAACTGGACAAATAGTGGCAGCGCTTTCAACAGCTAAATATAAGAACTGGCCTG AATCTGTGTATTTTTTCAAGAGAGGTGGCAGCATTCAGCAGTATATTTATAAACAGGAACCTGTACAGAA GTGCCCTGGAAGAAGGCCTGCTCTAAATTATCCAGTGTATGGAGAAACGACACAGGTTAGGAGACGTCGC TTTGAACGTGCTATAGGACCTTCTCAAACACACACCATCAGAATTCAATATTCACCTGCCAGACTGGCTT ATCAAGACAAAGGTGTCCTTCATAATGAAGTTAAAGTGAGTATACTGTGGAGAGGACTTCCAAATGTGGT TACCTCAGCTATATCACTGCCCAACATCAGAAAACCTGACGGCTATGATTACTATGCCTTTTCTAAAGAT CAATACTATAACATTGATGTGCCTAGTAGAACAGCAAGAGCAATTACTACTCGTTCTGGGCAGACCTTAT CCAAAGTCTGGTACAACTGTCCTTAG |
Restriction Sites | SgfI-MluI |
ACCN | NM_001303232 |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001303232.1, NP_001290161.1 |
RefSeq Size | 4921 bp |
RefSeq ORF | 4086 bp |
Locus ID | 10216 |
UniProt ID | Q92954 |
Cytogenetics | 1q31.1 |
Gene Summary | The protein encoded by this gene is a large proteoglycan that is synthesized by chondrocytes located at the surface of articular cartilage and by some synovial lining cells. This protein contains both chondroitin sulfate and keratan sulfate glycosaminoglycans. It functions as a boundary lubricant at the cartilage surface and contributes to the elastic absorption and energy dissipation of synovial fluid. Mutations in this gene result in camptodactyly-arthropathy-coxa vara-pericarditis syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] Transcript Variant: This variant (E, also known as V1) lacks an alternate in-frame exon in the 5' coding region, compared to variant A. The resulting protein (isoform E) is shorter than isoform A. |
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