POLR1B (NM_001282772) Human Untagged Clone

CAT#: SC337916

POLR1B (untagged) - Human polymerase (RNA) I polypeptide B, 128kDa (POLR1B), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: POLR1B
• Synonyms: A135; RPA2; RPA135; Rpo1-2; TCS4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001282772, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGAGCCAAAGAGGCTGCGGAAACGGGACTGCGGCCACTACTTCCGGCGTGTACCGAGAGACTGGCGT
  CCGGCGTGTACCGAGAGACTGGCGTCCGGTGTGCAGGTGGCCACATGGATCCTGGCAGCCGGTGGCGGAA
  CCTGCCCAGCGGGCCTAGCCTAAAGCACTTGACTGACCCCTCTTATGGAATCCCGCGGGAACAGCAAAAG
  GCAGCGTTGCAGGAGCTGACGCGGGCGCACGTGGAGTCCTTCAACTACGCTGTGCACGAGGGTCTCGGCC
  TCGCGGTGCAGGCTATACCTCCCTTTGAATTTGCTTTCAAAGATGAGCGTATCTCTTTTACTATTCTGGA
  TGCTGTTATCAGTCCACCTACAGTTCCAAAAGGGACCATCTGCAAAGAGGCCAATGTTTATCCAGCAGAA
  TGCCGGGGCCGAAGGAGTACCTACCGTGGGAAGTTGACAGCTGATATCAACTGGGCAGTGAATGGAATCT
  CAAAAGGAATCATTAAGCAGTTTCTTGGCTATGTTCCCATCATGGTGAAATCCAAGCTTTGCAACTTACG
  TAACCTTCCCCCACAAGCCCTCATTGAGCACCATGAGGAGGCAGAGGAAATGGGGGGCTATTTTATAATC
  AATGGCATTGAAAAAGTCATCCGAATGTTGATTATGCCTCGGAGAAATTTTCCCATTGCAATGATAAGAC
  CAAAATGGAAAACCAGAGGGCCTGGTTATACTCAGTATGGAGTTTCAATGCACTGTGTGAGGGAAGAACA
  TTCCGCTGTCAATATGAACCTCCACTACTTGGAAAATGGCACTGTTATGTTGAACTTTATTTACCGAAAA
  GAACTGTTCTTTCTTCCTTTGGGATTTGCACTTAAGGCACTTGTCAGCTTTTCTGATTATCAGATCTTTC
  AGGAGCTCATCAAAGGAAAAGAGGATGATTCTTTCCTTAGGAACTCTGTTTCTCAGATGTTAAGGATTGT
  AATGGAAGAGGGTTGTTCGACACAAAAACAGGTCCTTAACTACCTAGGTGAATGCTTCAGAGTAAAACTC
  AATGTTCCTGACTGGTACCCAAATGAGCAAGCTGCGGAGTTCCTGTTTAACCAGTGCATCTGTATCCACT
  TGAAATCCAATACTGAAAAGTTTTATATGCTTTGTCTCATGACGCGAAAGCTCTTTGCTTTAGCCAAAGG
  AGAGTGCATGGAGGACAATCCTGATAGTTTGGTGAACCAGGAAGTCCTCACACCGGGTCAGCTCTTCCTT
  ATGTTCCTGAAGGAAAAACTGGAAGGTTGGTTAGTGTCTATTAAAATAGCTTTTGATAAGAAGGCTCAGA
  AGACCAGTGTTTCCATGAACACTGACAATTTGATGAGGATTTTTACAATGGGCATAGACCTTACAAAACC
  ATTTGAATACCTTTTTGCTACTGGGAATCTGCGTTCTAAAACAGGTCTTGGCCTCCTACAAGATTCTGGA
  CTTTGTGTTGTGGCTGACAAGCTGAACTTCATACGCTACCTCTCCCATTTCCGCTGCGTGCACAGAGGGG
  CTGATTTTGCCAAGATGAGGACCACCACAGTACGCAGGCTGCTGCCAGAGTCCTGGGGCTTCCTTTGTCC
  CGTGCATACCCCAGACGGGGAGCCCTGTGGCCTGATGAACCACCTAACTGCCGTATGTGAGGTTGTCACA
  CAGTTTGTGTATACGGCATCTATTCCAGCTTTACTGTGCAACTTGGGGGTCACTCCCATTGATGGAGCTC
  CCCACCGATCATACAGTGAGTGCTACCCTGTCCTGCTGGACGGTGTCATGGTTGGCTGGGTGGATAAGGA
  TCTTGCTCCAGGCATCGCAGATTCTCTTCGTCATTTTAAGGTGTTGAGAGAGAAAAGAATTCCTCCCTGG
  ATGGAAGTGGTCCTTATACCCATGACAGGAAAACCAAGTCTGTACCCAGGATTGTTCCTTTTTACCACTC
  CTTGTAGACTGGTACGGCCTGTGCAGAACTTAGCATTGGGCAAAGAAGAGCTAATTGGAACTATGGAACA
  GATCTTCATGAATGTCGCTATCTTTGAGGATGAAGTTTTTGCTGGAGTTACCACACACCAGGAACTCTTT
  CCACACAGCCTGCTGAGTGTGATTGCCAACTTCATCCCTTTCTCTGATCACAACCAGAGTCCACGGAACA
  TGTACCAATGCCAGATGGGTAAGCAAACTATGGGCTTTCCACTTCTCACTTATCAAGACCGATCGGATAA
  CAAACTGTATCGTCTTCAGACTCCTCAGAGTCCCTTGGTGAGACCCTCCATGTATGATTATTATGACATG
  GATAACTATCCAATTGGGACCAATGCCATCGTTGCTGTGATTTCTTACACTGGCTATGATATGGAAGATG
  CCATGATTGTGAATAAGGCCTCTTGGGAACGAGGCTTTGCCCATGGAAGTGTCTACAAGTCTGAGTTCAT
  AGACCTCTCTGAAAAAATTAAACAAGGAGATAGTAGCCTGGTGTTTGGCATCAAACCTGGTGACCCACGC
  GTTCTGCAGAAGTTAGATGACGATGGATTGCCGTTTATAGGAGCAAAACTGCAGTACGGAGATCCGTATT
  ACAGCTACCTCAACCTCAACACCGGGGAAAGTTTTGTGATGTACTATAAGAGTAAAGAAAATTGTGTTGT
  GGATAACATCAAAGTGTGCAGTAATGACACTGGGAGTGGAAAATTCAAGTGTGTTTGCATCACTATGAGA
  GTGCCTCGGAACCCAACTATCGGAGATAAATTTGCCAGTCGCCATGGGCAGAAGGGCATTTTAAGCAGAT
  TGTGGCCGGCTGAGGACATGCCTTTTACTGAGAGTGGGATGGTCCCAGACATTCTGTTCAATCCCCATGG
  TTTTCCATCCCGCATGACCATTGGGATGTTAATTGAGAGTATGGCCGGGAAGTCTGCAGCTTTGCATGGT
  CTCTGCCATGATGCTACACCCTTCATCTTCTCAGAGGAGAACTCGGCCTTAGAATACTTTGGTGAGATGT
  TAAAGGCTGCTGGCTACAATTTCTATGGCACCGAGAGGTTATATAGTGGCATCAGTGGGCTAGAACTGGA
  AGCAGACATCTTCATAGGAGTGGTTTATTATCAGCGCTTACGCCATATGGTCTCAGACAAATTTCAAGTA
  AGGACAACTGGAGCCCGAGACAGAGTCACCAACCAGCCTATTGGGGGAAGAAATGTCCAGGGTGGAATCC
  GTTTTGGGGAGATGGAACGGGATGCGCTTTTAGCTCATGGTACATCTTTTCTCCTTCATGACCGCCTCTT
  CAACTGCTCAGATCGGTCGGTAGCCCATGTGTGTGTGAAGTGTGGCAGTTTACTCTCTCCACTGTTGGAG
  AAGCCACCCCCTTCTTGGTCTGCCATGCGCAACAGAAAATACAACTGTACTCTGTGTAGTCGCAGTGACA
  CTATCGATACTGTTTCTGTGCCTTATGTTTTTCGGTATTTTGTAGCTGAACTGGCAGCTATGAACATCAA
  AGTGAAACTGGATGTTGTTTAA
  
  
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001282772
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001282772.1, NP_001269701.1
• RefSeq Size: 5077 bp
• RefSeq ORF: 3522 bp
• Locus ID: 84172
• UniProt ID: Q9H9Y6
• Cytogenetics: 2q14.1
• Protein Families: Transcription Factors
• Protein Pathways: Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase
• Gene Summary: Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and Grummt, 1996 [PubMed 8921381]).[supplied by OMIM, Mar 2008]
  Transcript Variant: This variant (3) contains alternate 5' exon structure and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (3) has a distinct and longer N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.