MMS19 (NM_001289405) Human Untagged Clone

CAT#: SC337790

MMS19 (untagged) - Human MMS19 nucleotide excision repair homolog (S. cerevisiae) (MMS19), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MMS19
• Synonyms: CIAO4; hMMS19; MET18; MMS19L
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001289405, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCGCTGCCGCGGCTGTGGAGGCGGCGGCGCCTATGGGTGCCCTATGGGGCCTCGTGCACGACTTCG
  TCGTGGGTCAGCAAGAGGGCCCCGCTGACCAGGTGGCTGCAGATGTGAAATCTGGCAACTATACAGTGTT
  ACAAGTTGTGGAAGCCCTTGGGTCCTCTCTAGAGAATCCAGAACCCCGAACTCGGGCACGAGCAATCCAG
  CTTTTGTCACAGGTGCTACTCCACTGTCACACCTTGCTCCTGGAGAAGGAAGTGGTACACCTGATACTGT
  TCTATGAGAACCGGCTGAAGGACCATCATCTTGTGATCCCATCTGTCCTGCAGGGTTTGAAGGCACTTAG
  CCTGTGTGTGGCCCTGCCCCCAGGGCTGGCTGTTTCTGTGCTTAAAGCCATCTTCCAGGAAGTGCATGTA
  CAGTCCCTGCCACAGGTGGACCGACACACAGTCTACAATATCATCACCAATTTTATGCGAACCCGGGAAG
  AAGAGCTAAAGAGCCTAGGAGCTGACTTCACCTTTGGCTTCATCCAGGTGATGGATGGGGAAAAGGATCC
  CCGTAATCTTCTGGTGGCCTTCCGCATCGTCCATGACCTCATCTCCAGGGACTATAGCCTGGGACCCTTT
  GTGGAGGAGTTGTTTGAAGTGACATCCTGTTATTTCCCTATCGATTTTACCCCTCCACCTAATGATCCCC
  ATGGTATCCAGAGAGAAGACCTCATCCTGAGTCTTCGCGCTGTGCTGGCTTCTACACCACGATTTGCTGA
  GTTTCTGCTGCCCCTGTTGATTGAGAAAGTGGATTCTGAGGTTCTGAGTGCCAAGTTGGATTCTCTACAG
  ACTCTGAATGCTTGCTGTGCTGTGTATGGACAGAAGGAACTGAAGGACTTCCTCCCCAGCCTTTGGGCTT
  CTATCCGCAGAGAGGTGTTCCAGACGGCAAGTGAGCGGGTGGAGGCAGAGGGCCTGGCGGCCCTCCACTC
  CCTGACTGCGTGTTTGTCTCGCTCTGTGCTGAGGGCTGATGCTGAGGACCTCCTTGACTCCTTCCTTAGC
  AACATTCTACAGGACTGCAGGCACCACCTGTGTGAACCGGACATGAAACTGGTGTGGCCTAGTGCCAAGC
  TGTTGCAGGCAGCTGCAGGTGCATCTGCCCGGGCCTGTGACTCTGTCACCAGCAATGTACTGCCTTTACT
  GCTGGAACAGTTCCACAAGCACAGTCAGAGCAGCCAGCGGCGGACAATCCTTGAAATGCTCCTGGGTTTC
  TTGAAGCTGCAGCAGAAATGGAGCTATGAAGACAAAGATCAAAGGCCTCTGAATGGCTTCAAGGACCAGC
  TGTGCTCACTGGTATTCATGGCTCTAACAGACCCCAGCACCCAGCTTCAGCTTGTTGGCATCCGTACACT
  GACAGTCTTGGGTGCCCAGCCAGATCTCCTATCTTATGAGGACTTGGAGCTGGCAGTGGGTCACCTGTAC
  AGACTGAGCTTCCTGAAGGAGGATTCCCAGAGTTGCAGGGTGGCAGCACTGGAAGCATCAGGAACCCTGG
  CTGCTCTCTACCCTGTGGCCTTCAGCAGCCACCTCGTACCCAAGCTCGCTGAGGAGCTGCGTGTAGGGGA
  GTCAAATTTGACTAACGGAGATGAGCCCACCCAATGCTCCCGGCATCTGTGCTGTCTGCAAGCCTTGTCA
  GCTGTATCAACACATCCCAGCATCGTCAAGGAGACACTGCCTCTGCTGCTGCAGCATCTCTGGCAAGTGA
  ACAGAGGGAATATGGTTGCACAATCCAGTGACGTTATTGCTGTCTGTCAGAGCCTCAGACAGATGGCAGA
  AAAATGTCAGCAGGACCCTGAGAGTTGCTGGTATTTCCACCAGACAGCTATACCTTGCCTGCTTGCCTTG
  GCTGTGCAGGCCTCTATGCCAGAGAAGGAGCCCTCAGTTCTGAGAAAAGTACTATTGGAGGATGAGGTGT
  TGGCTGCCATGGTGTCTGTCATTGGCACTGCTACAACCCACCTGAGCCCTGAGTTAGCTGCCCAGAGTGT
  GACACACATTGTGCCCCTCTTCTTGGATGGCAACGTGTCCTTTCTGCCTGAAAACAGCTTCCCGAGCAGA
  TTCCAGCCATTCCAGGATGGCTCCTCAGGGCAGAGGCGGCTGATTGCACTGCTTATGGCCTTTGTCTGCT
  CCCTGCCTCGAAATGTGGAAATCCCTCAGCTGAACCAACTCATGCGGGAGCTTTTGGAACTGAGCTGCTG
  CCACAGCTGCCCCTTTTCTTCCACCGCTGCTGCCAAGTGCTTTGCAGGACTCCTCAACAAGCACCCTGCA
  GGGCAGCAGCTGGATGAATTCCTACAGCTAGCTGTGGACAAAGTGGAGGCTGGCCTGGGCTCTGGGCCCT
  GTCGTAGTCAGGCCTTCACTCTTCTTCTCTGGGTAACAAAGGCCCTAGTGCTCAGATACCATCCTCTCAG
  CTCCTGCCTTACAGCCCGGCTCATGGGCCTCCTGAGTGACCCAGAATTAGGTCCAGCAGCAGCTGATGGC
  TTCTCTCTGCTCATGTCTGACTGCACTGATGTGCTGACTCGTGCTGGCCATGCCGAAGTGCGGATCATGT
  TCCGCCAGCGGTTCTTCACAGATAATGTGCCTGCTTTGGTCCAGGGCTTCCATGCTGCTCCCCAAGATGT
  GAAGCCAAACTACTTGAAGGGTCTTTCTCATGTACTTAACAGGCTGCCCAAGCCTGTACTCTTGCCAGAG
  CTGCCCACGCTTCTTTCCTTGCTGCTGGAGGCCCTGTCCTGCCCTGACTGTGTGGTGCAGCTCTCCACCC
  TCAGCTGCCTTCAGCCTCTTCTACTGGAAGCACCCCAAGTCATGAGTCTTCACGTGGACACCCTCGTCAC
  CAAGTTTCTGAACCTCAGCTCTAGCCCTTCCATGGCTGTCCGGATCGCCGCACTGCAGTGCATGCATGCT
  CTCACTCGCCTGCCCACCCCTGTGCTGCTGCCGTACAAACCACAGGTGATTCGGGCCTTAGCCAAACCCC
  TGGATGACAAGAAGAGACTGGTGCGCAAGGAAGCAGTGTCAGCCAGAGGGGAGTGGTTTCTGTTGGGGAG
  CCCTGGCAGCTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289405
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289405.1, NP_001276334.1
• RefSeq Size: 3585 bp
• RefSeq ORF: 3093 bp
• Locus ID: 64210
• UniProt ID: Q96T76
• Cytogenetics: 10q24.1
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: Key component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into apoproteins specifically involved in DNA metabolism and genomic integrity. In the CIA complex, MMS19 acts as an adapter between early-acting CIA components and a subset of cellular target iron-sulfur proteins such as ERCC2/XPD, FANCJ and RTEL1, thereby playing a key role in nucleotide excision repair (NER), homologous recombination-mediated double-strand break DNA repair, DNA replication and RNA polymerase II (POL II) transcription (PubMed:22678362, PubMed:22678361, PubMed:29225034, PubMed:23585563). As part of the mitotic spindle-associated MMXD complex, plays a role in chromosome segregation, probably by facilitating iron-sulfur cluster assembly into ERCC2/XPD (PubMed:20797633). Indirectly acts as a transcriptional coactivator of estrogen receptor (ER), via its role in iron-sulfur insertion into some component of the TFIIH-machinery (PubMed:11279242).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) differs in the 5' UTR, compared to variant 1. Variants 1 and 4 encode the same protein (isoform 1). Sequence Note: A downstream start codon is selected for this RefSeq based on better conservation with homologous proteins. The use of an alternative upstream start codon, which is present in some species, including human, chimp and macaque (with a subsequent CDS frameshift in the latter species), would increase the protein length from 1030 aa to 1051 aa. The shorter protein is referred to in the literature, including PMIDs 11071939, 11279242 and 11328871.