LIMCH1 (NM_001289124) Human Untagged Clone

CAT#: SC337661

LIMCH1 (untagged) - Human LIM and calponin homology domains 1 (LIMCH1), transcript variant 7

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LIMCH1
• Synonyms: LIMCH1A; LMO7B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001289124, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCGAAAGGACACTGATGACATTGAAAGTCCTAAACGCAGTATCCGAGACAGTGGCTACATCGACTGCT
  GGGATTCCGAGCGCAGCGACTCCCTCTCTCCTCCTCGCCACGGCAGAGATGATTCCTTCGACAGCCTGGA
  TTCCTTTGGCTCTCGCTCTCGGCAGACGCCTTCACCAGATGTAGTCCTCAGGGGAAGCAGCGATGGGAGA
  GGAAGCGACTCTGAATCCGACTTGCCTCATCGGAAGCTGCCAGATGTGAAGAAGGATGACATGTCTGCAC
  GGCGGACTTCCCATGGTGAGCCGAAATCAGCAGTGCCTTTTAACCAGTACCTCCCGAACAAAAGCAATCA
  GACGGCCTACGTCCCCGCGCCTCTGAGAAAGAAGAAAGCAGAGAGAGAGGAATACCGCAAGAGCTGGAGT
  ACCGCCACCTCCCCGCTGGGTGGGGAGAGGCCCTTCAGGAGCACCAGCATGTTTGACATGCGGTGTGAGG
  AGGAGGCCGCGGTGCAGCCGCACAGCAGGGCCCGCCAGGAGCAGCTGCAGCTGATAAATAACCAGCTGAG
  GGAAGAGGACGACAAATGGCAAGATGACCTGGCTCGTTGGAAGAGTCGTAGAAGAAGTGTTTCTCAGGAC
  TTAATCAAGAAAGAGGAAGAAAGGAAAAAAATGGAGAAGTTACTGGCTGGAGAAGATGGGACAAGTGAAC
  GAAGGAAAAGCATCAAAACCTACAGAGAAATTGTTCAAGAAAAAGAGCGGAGAGAGAGAGAGCTGCATGA
  AGCATATAAGAACGCTCGGTCCCAGGAGGAGGCAGAGGGGATCCTTCAACAGTACATTGAGAGGTTCACC
  ATCAGTGAGGCTGTTCTCGAACGCTTGGAGATGCCAAAAATTCTGGAAAGAAGCCATTCAACAGAGCCAA
  ATTTATCCTCCTTCCTGAATGACCCCAATCCCATGAAATACCTGCGGCAACAGTCACTGCCTCCACCCAA
  ATTCACTGCCACTGTTGAAACCACCATTGCTCGTGCCAGTGTTCTGGATACCAGCATGTCAGCAGGCAGT
  GGGTCTCCAAGCAAAACTGTCACTCCCAAAGCAGTGCCTATGCTGACACCCAAGCCTTACTCCCAGCCCA
  AAAATTCTCAAGATGTTCTGAAGACCTTTAAGGTAGACGGGAAAGTCAGTGTGAATGGAGAGACGGTTCA
  TAGAGAGGAGGAGAAGGAAAGAGAGTGTCCCACGGTGGCACCTGCCCACTCCTTAACCAAATCCCAGATG
  TTTGAAGGTGTGGCCAGAGTGCACGGGTCTCCACTGGAGCTGAAACAAGACAACGGTAGCATCGAGATCA
  ACATAAAGAAGCCAAACTCTGTTCCCCAAGAGCTCGCAGCAACCACTGAGAAAACGGAACCGAATAGTCA
  AGAGGACAAGAATGATGGTGGAAAATCAAGAAAAGGGAATATAGAACTTGCCTCATCAGAACCACAGCAT
  TTTACAACAACTGTGACTCGATGCAGCCCGACCGTGGCCTTTGTGGAATTTCCCTCCAGCCCCCAGCTGA
  AGAATGATGTGTCGGAAGAAAAAGACCAGAAGAAACCAGAAAATGAAATGAGTGGAAAGGTGGAGTTGGT
  GCTGTCACAAAAGGTGGTAAAGCCAAAATCTCCAGAACCCGAAGCAACGCTGACATTTCCATTTCTGGAC
  AAAATGCCTGAAGCCAACCAACTACATTTGCCAAATCTCAATTCTCAAGAGTCGCCTGGCACTGCCAGTG
  TTCCGCTTAGAGTTCAGAACTCTTGGCGACGATCCCAGTTTTTCTCCCAGTCAGTGGATTCTCCAAGCAG
  TGAGAAGTCACCTGTTATGACACCTTTTAAGTTCTGGGCATGGGACCCAGAAGAGGAGCGCAGGCGACAG
  GAAAAATGGCAACAGGAACAGGAACGTTTGCTCCAGGAGAGATACCAGAAGGAGCAGGACAAGCTGAAAG
  AAGAGTGGGAAAAGGCCCAAAAGGAGGTGGAAGAGGAAGAACGCAGATACTATGAGGAGGAGCGTAAGAT
  AATTGAAGACACTGTGGTTCCATTTACTGTTTCTTCAAGTTCCGCTGACCAGCTGTCTACCTCTTCCTCC
  ATGACTGAAGGCAGTGGGACAATGAATAAGATAGACCTGGGAAACTGTCAAGATGAAAAACAAGACAGAA
  GATGGAAGAAATCATTCCAGGGAGATGACAGTGACTTATTGCTGAAGACTAGGGAAAGTGATCGACTGGA
  GGAGAAGGGCAGCCTAACTGAAGGGGCCTTGGCTCATTCTGGGAACCCTGTATCAAAAGGAGTCCATGAA
  GACCATCAGCTGGATACCGAGGCTGGGGCCCCACACTGTGGAACAAACCCACAGCTTGCTCAGGATCCAT
  CCCAGAATCAGCAGACATCAAATCCAACGCACAGTTCAGAAGATGTGAAGCCAAAAACCCTCCCGCTGGA
  TAAAAGCATTAACCATCAGATCGAGTCTCCCAGTGAAAGGCGGAAGTCTATAAGTGGAAAGAAGCTGTGC
  TCTTCCTGTGGGCTTCCTTTGGGTAAAGGAGCTGCAATGATCATCGAGACCCTCAATCTCTATTTTCACA
  TCCAGTGTTTCAGGTGTGGAATTTGTAAAGGCCAGCTTGGAGATGCAGTGAGTGGGACGGATGTTAGGAT
  TCGAAATGGTCTCCTGAACTGTAATGATTGCTACATGCGATCCAGAAGTGCCGGGCAGCCTACAACATTG
  TGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289124
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289124.1, NP_001276053.1
• RefSeq Size: 6187 bp
• RefSeq ORF: 2733 bp
• Locus ID: 22998
• UniProt ID: Q9UPQ0
• Cytogenetics: 4p13
• Gene Summary: Actin stress fibers-associated protein that activates non-muscle myosin IIa. Activates the non-muscle myosin IIa complex by promoting the phosphorylation of its regulatory subunit MRLC/MYL9. Through the activation of non-muscle myosin IIa, positively regulates actin stress fibers assembly and stabilizes focal adhesions. It therefore negatively regulates cell spreading and cell migration.[UniProtKB/Swiss-Prot Function]