MMS19 (NM_001289404) Human Untagged Clone

CAT#: SC337608

MMS19 (untagged) - Human MMS19 nucleotide excision repair homolog (S. cerevisiae) (MMS19), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MMS19
• Synonyms: CIAO4; hMMS19; MET18; MMS19L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001289404, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCGAACCCGGGAAGAAGAGCTAAAGAGCCTAGGAGCTGACTTCACCTTTGGCTTCATCCAGGTGATGG
  ATGGGGAAAAGGATCCCCGTAATCTTCTGGTGGCCTTCCGCATCGTCCATGACCTCATCTCCAGGGACTA
  TAGCCTGGGACCCTTTGTGGAGGAGTTGTTTGAAGTGACATCCTGTTATTTCCCTATCGATTTTACCCCT
  CCACCTAATGATCCCCATGGTATCCAGAGAGAAGACCTCATCCTGAGTCTTCGCGCTGTGCTGGCTTCTA
  CACCACGATTTGCTGAGTTTCTGCTGCCCCTGTTGATTGAGAAAGTGGATTCTGAGGTTCTGAGTGCCAA
  GTTGGATTCTCTACAGACTCTGAATGCTTGCTGTGCTGTGTATGGACAGAAGGAACTGAAGGACTTCCTC
  CCCAGCCTTTGGGCTTCTATCCGCAGAGAGGTGTTCCAGACGGCAAGTGAGCGGGTGGAGGCAGAGGGCC
  TGGCGGCCCTCCACTCCCTGACTGCGTGTTTGTCTCGCTCTGTGCTGAGGGCTGATGCTGAGGACCTCCT
  TGACTCCTTCCTTAGCAACATTCTACAGGACTGCAGGCACCACCTGTGTGAACCGGACATGAAACTGGTG
  TGGCCTAGTGCCAAGCTGTTGCAGGCAGCTGCAGGTGCATCTGCCCGGGCCTGTGACTCTGTCACCAGCA
  ATGTACTGCCTTTACTGCTGGAACAGTTCCACAAGCACAGTCAGAGCAGCCAGCGGCGGACAATCCTTGA
  AATGCTCCTGGGTTTCTTGAAGCTGCAGCAGAAATGGAGCTATGAAGACAAAGATCAAAGGCCTCTGAAT
  GGCTTCAAGGACCAGCTGTGCTCACTGGTATTCATGGCTCTAACAGACCCCAGCACCCAGCTTCAGCTTG
  TTGGCATCCGTACACTGACAGTCTTGGGTGCCCAGCCAGATCTCCTATCTTATGAGGACTTGGAGCTGGC
  AGTGGGTCACCTGTACAGACTGAGCTTCCTGAAGGAGGATTCCCAGAGTTGGGTGGCAGCACTGGAAGCA
  TCAGGAACCCTGGCTGCTCTCTACCCTGTGGCCTTCAGCAGCCACCTCGTACCCAAGCTCGCTGAGGAGC
  TGCGTGTAGGGGAGTCAAATTTGACTAACGGAGATGAGCCCACCCAATGCTCCCGGCATCTGTGCTGTCT
  GCAAGCCTTGTCAGCTGTATCAACACATCCCAGCATCGTCAAGGAGACACTGCCTCTGCTGCTGCAGCAT
  CTCTGGCAAGTGAACAGAGGGAATATGGTTGCACAATCCAGTGACGTTATTGCTGTCTGTCAGAGCCTCA
  GACAGATGGCAGAAAAATGTCAGCAGGACCCTGAGAGTTGCTGGTATTTCCACCAGACAGCTATACCTTG
  CCTGCTTGCCTTGGCTGTGCAGGCCTCTATGCCAGAGAAGGAGCCCTCAGTTCTGAGAAAAGTACTATTG
  GAGGATGAGGTGTTGGCTGCCATGGTGTCTGTCATTGGCACTGCTACAACCCACCTGAGCCCTGAGTTAG
  CTGCCCAGAGTGTGACACACATTGTGCCCCTCTTCTTGGATGGCAACGTGTCCTTTCTGCCTGAAAACAG
  CTTCCCGAGCAGATTCCAGCCATTCCAGGATGGCTCCTCAGGGCAGAGGCGGCTGATTGCACTGCTTATG
  GCCTTTGTCTGCTCCCTGCCTCGAAATGTGGAAATCCCTCAGCTGAACCAACTCATGCGGGAGCTTTTGG
  AACTGAGCTGCTGCCACAGCTGCCCCTTTTCTTCCACCGCTGCTGCCAAGTGCTTTGCAGGACTCCTCAA
  CAAGCACCCTGCAGGGCAGCAGCTGGATGAATTCCTACAGCTAGCTGTGGACAAAGTGGAGGCTGGCCTG
  GGCTCTGGGCCCTGTCGTAGTCAGGCCTTCACTCTTCTTCTCTGGGTAACAAAGGCCCTAGTGCTCAGAT
  ACCATCCTCTCAGCTCCTGCCTTACAGCCCGGCTCATGGGCCTCCTGAGTGACCCAGAATTAGGTCCAGC
  AGCAGCTGATGGCTTCTCTCTGCTCATGTCTGACTGCACTGATGTGCTGACTCGTGCTGGCCATGCCGAA
  GTGCGGATCATGTTCCGCCAGCGGTTCTTCACAGATAATGTGCCTGCTTTGGTCCAGGGCTTCCATGCTG
  CTCCCCAAGATGTGAAGCCAAACTACTTGAAGGGTCTTTCTCATGTACTTAACAGGCTGCCCAAGCCTGT
  ACTCTTGCCAGAGCTGCCCACGCTTCTTTCCTTGCTGCTGGAGGCCCTGTCCTGCCCTGACTGTGTGGTG
  CAGCTCTCCACCCTCAGCTGCCTTCAGCCTCTTCTACTGGAAGCACCCCAAGTCATGAGTCTTCACGTGG
  ACACCCTCGTCACCAAGTTTCTGAACCTCAGCTCTAGCCCTTCCATGGCTGTCCGGATCGCCGCACTGCA
  GTGCATGCATGCTCTCACTCGCCTGCCCACCCCTGTGCTGCTGCCGTACAAACCACAGGTGATTCGGGCC
  TTAGCCAAACCCCTGGATGACAAGAAGAGACTGGTGCGCAAGGAAGCAGTGTCAGCCAGAGGGGAGTGGT
  TTCTGTTGGGGAGCCCTGGCAGCTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289404
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289404.1, NP_001276333.1
• RefSeq Size: 3639 bp
• RefSeq ORF: 2616 bp
• Locus ID: 64210
• UniProt ID: Q96T76
• Cytogenetics: 10q24.1
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: Key component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into apoproteins specifically involved in DNA metabolism and genomic integrity. In the CIA complex, MMS19 acts as an adapter between early-acting CIA components and a subset of cellular target iron-sulfur proteins such as ERCC2/XPD, FANCJ and RTEL1, thereby playing a key role in nucleotide excision repair (NER), homologous recombination-mediated double-strand break DNA repair, DNA replication and RNA polymerase II (POL II) transcription (PubMed:22678362, PubMed:22678361, PubMed:29225034, PubMed:23585563). As part of the mitotic spindle-associated MMXD complex, plays a role in chromosome segregation, probably by facilitating iron-sulfur cluster assembly into ERCC2/XPD (PubMed:20797633). Indirectly acts as a transcriptional coactivator of estrogen receptor (ER), via its role in iron-sulfur insertion into some component of the TFIIH-machinery (PubMed:11279242).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation from a downstream start codon, compared to variant 1. This results in an isoform (3) that has a shorter N-terminus, compared to isoform 1.