TLE2 (NM_001300846) Human Untagged Clone

CAT#: SC337353

TLE2 (untagged) - Human transducin-like enhancer of split 2 (TLE2), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TLE2
• Synonyms: ESG; ESG2; GRG2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001300846, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTACCCCCAGGGAAGGCACCCGACCCCGCTCCAGTCCGGCCAGCCCTTCAAGTTCTCGATCTTGGAGA
  TCTGCGACCGCATCAAAGAAGAATTCCAGTTTCTTCAGGCTCAATACCACAGCCTCAAGCTAGAATGTGA
  GAAGCTGGCCAGCGAGAAGACGGAAATGCAGCGACATTATGTCATGTATTATGAGATGTCGTACGGGCTC
  AACATTGAAATGCATAAGCAGGCGGAGATTGTGAAGCGTCTGAGCGGTATCTGCGCTCAGATTATCCCCT
  TCCTGACCCAGGAGCATCAGCAGCAGGTGCTCCAGGCCGTAGAACGCGCCAAGCAGGTCACCGTGGGGGA
  GCTGAACAGCCTCATCGGGCAGCAGCAGCTCCAGCCGCTGTCCCACCACGCACCCCCTGTGCCCCTCACC
  CCCCGCCCAGCCGGGCTGGTGGGCGGCAGTGCTACGGGGCTGCTTGCTCTGTCTGGAGCCCTGGCTGCCC
  AGGCTCAGCTGGCGGCGGCTGTCAAGGAGGACCGTGCGGGCGTGGAGGCCGAGGGGTCCAGAGTGGAGAG
  AGCCCCGAGCAGGAGTGCATCTCCCTCGCCCCCTGAGAGTCTCGTGGAGGAGGAGCGACCGAGTGGCCCT
  GGTGGTGGCGGGAAGCAGAGAGCAGATGAGAAGGAGCCATCAGGACCTTATGAAAGCGACGAAGACAAGA
  GTGATTACAATCTGGTGGTGGACGAGGACCAACCCTCAGAGCCCCCCAGCCCGGCTACCACCCCCTGCGG
  AAAGGTACCCATCTGCATTCCTGCCCGTCGGGACCTGGTGGACAGTCCAGCCTCCTTGGCCTCTAGCCTT
  GGCTCACCGCTGCCTAGAGCCAAGGAGCTCATCCTGAATGACCTTCCCGCCAGCACTCCTGCCTCCAAAT
  CCTGTGACTCCTCCCCGCCCCAGGACGCTTCCACCCCCGGGCCCAGCTCGGCCAGTCACCTCTGCCAGCT
  TGCTGCCAAGCCAGCACCTTCCACGGACAGCGTCGCCCTGAGGAGCCCCCTGACTCTGTCCAGTCCCTTC
  ACCACGTCCTTCAGCCTGGGCTCCCACAGCACTCTCAACGGAGACCTCTCCGTGCCCAGCTCCTACGTCA
  GCCTCCACCTGTCCCCCCAGGTCAGCAGCTCTGTGGTGTACGGACGCTCCCCCGTGATGGCATTTGAGTC
  TCATCCCCATCTCCGAGGGTCATCCGTCTCTTCCTCCCTACCCAGCATCCCTGGGGGAAAGCCGGCCTAC
  TCCTTCCACGTGTCTGCGGACGGGCAGATGCAGCCGGTTCCCTTCCCCTCGGATGCACTGGTAGGCGCGG
  GCATCCCGCGGCACGCCCGGCAGCTGCACACGCTGGCCCATGGCGAGGTGGTCTGCGCGGTCACCATCAG
  CGGCTCCACACAGCATGTGTACACGGGCGGCAAGGGCTGTGTGAAGGTGTGGGACGTGGGCCAGCCTGGG
  GCCAAGACGCCCGTGGCCCAGCTCGACTGCCTGAACCGAGACAACTACATTCGTTCCTGCAAGTTGCTGC
  CGGATGGCCGGAGTCTGATCGTGGGCGGTGAGGCCAGCACCTTGTCCATTTGGGACCTGGCGGCGCCCAC
  CCCCCGTATCAAGGCCGAGCTGACTTCCTCAGCCCCAGCCTGCTACGCCCTGGCCGTCAGCCCCGACGCC
  AAGGTTTGCTTCTCCTGCTGCAGCGATGGCAACATTGTGGTCTGGGACCTGCAGAATCAGACTATGGTCA
  GGCAGTTCCAGGGCCACACGGACGGCGCCAGCTGCATTGATATTTCCGATTACGGCACTCGGCTCTGGAC
  AGGGGGCCTGGACAACACGGTGCGCTGCTGGGACCTGCGGGAGGGCCGCCAGCTGCAGCAGCATGACTTC
  AGCTCCCAGATTTTCTCCCTGGGCCACTGCCCTAACCAGGACTGGCTGGCGGTCGGAATGGAGAGTAGCA
  ACGTGGAGATCCTGCACGTCCGCAAGCCGGAGAAATACCAGCTGCACCTCCACGAGAGCTGCGTGCTGTC
  CCTGAAGTTTGCCTCCTGCGGACGGTGGTTTGTGAGCACCGGGAAGGACAACCTGCTCAACGCCTGGAGG
  ACGCCGTACGGGGCCAGCATTTTCCAGTCCAAGGAGTCGTCCTCAGTCCTGAGTTGTGACATCTCCAGAA
  ATAACAAATACATCGTGACAGGCTCGGGGGACAAGAAGGCCACCGTGTATGAGGTGGTCTACTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001300846
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001300846.1, NP_001287775.1
• RefSeq Size: 2759 bp
• RefSeq ORF: 2235 bp
• Locus ID: 7089
• UniProt ID: Q04725
• Cytogenetics: 19p13.3
• Protein Families: Transcription Factors
• Gene Summary: Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) represents the longest transcript and encodes the longest isoform (4).