NOLC1 (NM_001284389) Human Untagged Clone

CAT#: SC337241

NOLC1 (untagged) - Human nucleolar and coiled-body phosphoprotein 1 (NOLC1), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NOLC1
• Synonyms: NOPP130; NOPP140; NS5ATP13; P130; Srp40
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001284389, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGGACGCCGGCATTCGCCGCGTGGTTCCCAGCGACCTGTATCCCCTCGTGCTCGGCTTCCTGCGCG
  ATAACCAACTCTCAGAGGTGGCCAATAAGTTCGCCAAAGCGACAGGAGCTACACAGCAGGATGCCAATGC
  CTCTTCCCTCTTAGACATCTATAGCTTCTGGCTCAACAGGTCTGCCAAGGTCCCAGAGCGAAAGTTACAG
  GCAAATGGACCAGTGGCTAAGAAAGCTAAGAAGAAGGCCTCATCCAGTGACAGTGAGGACAGCAGCGAGG
  AGGAGGAGGAAGTTCAAGGGCCTCCAGCAAAGAAGGCTGCTGTACCTGCCAAGCGAGTCGGTCTGCCTCC
  TGGGAAGGCTGCAGCCAAAGCATCAGAGAGTAGCAGCAGTGAAGAGTCCAGTGATGATGATGATGAGGAG
  GACCAAAAGAAACAGCCTGTCCAGAAGGGAGTTAAGCCCCAAGCCAAGGCAGCCAAAGCTCCTCCTAAGA
  AGGCCAAGAGCTCTGATTCTGATTCTGACTCAAGCTCCGAGGATGAGCCACCAAAGAACCAGAAGCCAAA
  GATAACACCTGTGACAGTTAAAGCTCAGACTAAAGCCCCTCCCAAACCAGCTCGAGCAGCACCTAAAATA
  GCCAATGGTAAAGCAGCCAGTAGCAGCAGTAGCAGCAGCAGCAGCAGTAGCAGTGATGACTCAGAGGAGG
  AGAAGGCAGCAGCCACCCCCAAGAAGACTGTACCTAAAAAGCAAGTTGTGGCCAAGGCCCCAGTGAAAGC
  AGCTACCACCCCTACCCGGAAGAGTTCTAGCAGTGAGGATTCCTCCAGTGACGAGGAAGAGGAGCAAAAA
  AAACCCATGAAAAATAAACCAGGTCCCTACAGTTCAGTCCCCCCGCCTTCTGCTCCCCCACCAAAGAAGT
  CTCTGGGAACCCAGCCTCCCAAGAAGGCTGTGGAGAAGCAGCAGCCTGTGGAAAGCAGTGAAGACAGCAG
  TGATGAGTCTGATTCAAGTTCTGAAGAAGAGAAGAAACCCCCAACTAAGGCAGTAGTCTCTAAAGCAACC
  ACTAAACCACCTCCAGCAAAGAAAGCAGCAGAGAGCTCTTCAGACAGCTCAGACTCTGACAGCTCTGAGG
  ATGATGAAGCTCCTTCTAAGCCAGCTGGTACCACCAAGAATTCTTCAAATAAGCCAGCTGTCACCACCAA
  GTCACCTGCAGTGAAGCCAGCTGCAGCCCCCAAGCAACCTGTGGGCGGTGGCCAGAAGCTTCTGACGAGA
  AAGGCTGACAGCAGCTCCAGTGAGGAAGAGAGCAGCTCCAGTGAGGAGGAGAAGACAAAGAAGATGGTGG
  CCACCACTAAGCCCAAGGCGACTGCCAAAGCAGCTCTATCTCTGCCTGCCAAGCAGGCTCCTCAGGGTAG
  TAGGGACAGCAGCTCTGATTCAGACAGCTCCAGCAGTGAGGAGGAGGAAGAGAAGACATCTAAGTCTGCA
  GTTAAGAAGAAGCCACAGAAGGTAGCAGGAGGTGCAGCCCCTTCCAAGCCAGCCTCTGCAAAGAAAGGAA
  AGGCTGAGAGCAGCAACAGTTCTTCTTCTGATGACTCCAGTGAGGAAGAGGAAGAGAAGCTCAAGGGCAA
  GGGCTCTCCAAGACCACAAGCCCCCAAGGCCAATGGCACCTCTGCACTGACTGCCCAGAATGGAAAAGCA
  GCTAAGAACAGTGAGGAGGAGGAAGAAGAAAAGAAAAAGGCGGCAGTGGTAGTTTCCAAATCAGGTTCAT
  TAAAGAAGCGGAAGCAGAATGAGGCTGCCAAGGAGGCAGAGACTCCTCAGGCCAAGAAGATAAAGCTTCA
  GACCCCTAACACATTTCCAAAAAGGAAGAAAGGAGAAAAAAGGGCATCATCCCCATTCCGAAGGGTCAGG
  GAGGAGGAAATTGAGGTGGATTCACGAGTTGCGGACAACTCCTTTGATGCCAAGCGAGGTGCAGCCGGAG
  ACTGGGGAGAGCGAGCCAATCAGGTTTTGAAGTTCACCAAAGGCAAGTCCTTTCGGCATGAGAAAACCAA
  GAAGAAGCGGGGCAGCTACCGGGGAGGCTCAATCTCTGTCCAGGTCAATTCTATTAAGTTTGACAGCGAG
  TGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001284389
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001284389.1, NP_001271318.1
• RefSeq Size: 3950 bp
• RefSeq ORF: 2103 bp
• Locus ID: 9221
• UniProt ID: Q14978
• Cytogenetics: 10q24.32
• Protein Families: Stem cell - Pluripotency
• Gene Summary: Nucleolar protein that acts as a regulator of RNA polymerase I by connecting RNA polymerase I with enzymes responsible for ribosomal processing and modification (PubMed:10567578, PubMed:26399832). Required for neural crest specification: following monoubiquitination by the BCR(KBTBD8) complex, associates with TCOF1 and acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification (PubMed:26399832). Involved in nucleologenesis, possibly by playing a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus (PubMed:9016786). It has intrinsic GTPase and ATPase activities (PubMed:9016786).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) uses two alternate in-frame splice sites in the 5' coding region, compared to variant 1. This results in a shorter protein (isoform 3), compared to isoform 1.