SUV420h1 (KMT5B) (NM_001300908) Human Untagged Clone

CAT#: SC337085

SUV420H1 (untagged) - Human suppressor of variegation 4-20 homolog 1 (Drosophila) (SUV420H1), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SUV420h1
• Synonyms: CGI-85; CGI85; MRD51; SUV420H1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC337085 representing NM_001300908.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCTACTTAGACATGGAGAAAACGACTTCAGTGTCATGTACTCCACAAGGAAAAACTGTGCTCAACTC
  TGGCTGGGTCCTGCTGCGTTTATAAACCATGATTGCAGACCTAATTGTAAGTTTGTGTCAACTGGTCGA
  GATACAGCATGTGTGAAGGCTCTAAGAGACATTGAACCTGGAGAAGAAATTTCTTGTTATTATGGAGAT
  GGGTTCTTTGGAGAAAATAATGAGTTCTGCGAGTGTTACACTTGCGAAAGACGGGGCACTGGTGCTTTT
  AAATCCAGAGTGGGACTGCCTGCGCCTGCTCCTGTTATCAATAGCAAATATGGACTCAGAGAAACAGAT
  AAACGTTTAAATAGGCTTAAAAAGTTAGGTGACAGCAGCAAAAATTCAGACAGTCAATCTGTCAGCTCT
  AACACTGATGCAGATACCACTCAGGAAAAAAACAATGCAACTTCTAACCGAAAATCTTCAGTTGGCGTA
  AAAAAGAATAGCAAGAGCAGAACGTTAACGAGGCAATCTATGTCAAGAATTCCAGCTTCTTCCAACTCT
  ACCTCATCTAAGCTAACTCATATAAATAATTCCAGGGTACCAAAGAAACTGAAGAAGCCTGCAAAGCCT
  TTACTTTCAAAGATAAAATTGAGAAATCATTGCAAGCGGCTGGAGCAAAAGAATGCTTCAAGAAAACTC
  GAAATGGGAAACTTAGTACTGAAAGAGCCTAAAGTAGTTCTGTATAAAAATTTGCCCATTAAAAAAGAT
  AAGGAGCCAGAGGGACCAGCCCAAGCCGCAGTTGCCAGCGGGTGCTTGACTAGACACGCGGCGAGAGAA
  CACAGACAGAATCCTGTGAGAGGTGCTCATTCGCAGGGGGAGAGCTCGCCCTGCACCTACATAACTCGG
  CGGTCAGTGAGGACAAGAACAAATCTGAAGGAGGCCTCTGACATCAAGCTTGAACCAAATACGTTGAAT
  GGCTATAAAAGCAGTGTGACGGAACCTTGCCCCGACAGTGGTGAACAGCTGCAGCCAGCTCCTGTGCTG
  CAGGAGGAAGAACTGGCTCATGAGACTGCACAAAAAGGGGAGGCAAAGTGTCATAAGAGTGACACAGGC
  ATGTCCAAAAAGAAGTCACGACAAGGAAAACTTGTGAAACAGTTTGCAAAAATAGAGGAATCTACTCCA
  GTGCACGATTCTCCTGGAAAAGACGACGCGGTACCAGATTTGATGGGTCCCCATTCTGACCAGGGTGAG
  CACAGTGGCACTGTGGGCGTGCCTGTGAGCTACACAGACTGTGCTCCTTCACCCGTCGGTTGTTCAGTT
  GTGACATCAGATAGCTTCAAAACAAAAGACAGCTTTAGAACTGCAAAAAGTAAAAAGAAGAGGCGAATC
  ACAAGGTATGATGCACAGTTAATCCTAGAAAATAACTCTGGGATTCCCAAATTGACTCTTCGTAGGCGT
  CATGATAGCAGCAGCAAAACAAATGACCAAGAGAATGATGGAATGAACTCTTCCAAAATAAGCATCAAG
  TTAAGCAAAGACCATGACAACGATAACAATCTCTATGTAGCAAAGCTTAATAATGGATTTAACTCAGGA
  TCAGGCAGTAGTTCTACAAAATTAAAAATCCAGCTAAAACGAGATGAGGAAAATAGGGGGTCTTATACA
  GAGGGGCTTCATGAAAATGGGGTGTGCTGCAGTGATCCTCTTTCTCTCTTGGAGTCTCGAATGGAGGTG
  GATGACTATAGTCAGTATGAGGAAGAAAGTACAGATGATTCCTCCTCTTCTGAGGGCGATGAAGAGGAG
  GATGACTATGATGATGACTTTGAAGACGATTTTATTCCTCTTCCTCCAGCTAAGCGCTTGAGGTTAATA
  GTTGGAAAAGACTCTATAGATATTGACATTTCTTCAAGGAGAAGAGAAGATCAGTCTTTAAGGCTTAAT
  GCCTAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001300908
• Insert Size: 1938 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001300908.1
• RefSeq Size: 5802 bp
• RefSeq ORF: 1938 bp
• Locus ID: 51111
• Cytogenetics: 11q13.2
• Protein Families: Druggable Genome
• Protein Pathways: Lysine degradation
• MW: 71.6 kDa
• Gene Summary: This gene encodes a protein that contains a SET domain. SET domains appear to be protein-protein interaction domains that mediate interactions with a family of proteins that display similarity with dual-specificity phosphatases (dsPTPases). The function of this gene has not been determined. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]
  Transcript Variant: This variant (4) uses an alternate first exon and an alternate splice junction at the 5' end of an exon, and uses a downstream start codon compared to variant 1. The resulting isoform (4) is shorter at the N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.