ZNF189 (NM_001278232) Human Untagged Clone

CAT#: SC336975

ZNF189 (untagged) - Human zinc finger protein 189 (ZNF189), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ZNF189
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC336975 representing NM_001278232.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCTTCCCCGAGCCCCCCGCCGGAGTCGAAGGAGTGGGATTATCTGGACCCAGCTCAGAGAAGCCTG
  TATAAAGATGTCATGATGGAGAATTATGGAAACCTGGTCTCACTGGATGTTTTGAACAGAGATAAGGAT
  GAGGAGCCAACTGTAAAACAAGAGATTGAAGAAATTGAGGAAGAAGTGGAACCACAGGGTGTAATAGTT
  ACAAGAATCAAAAGTGAAATTGACCAGGATCCTATGGGTAGAGAAACATTTGAACTTGTTGGTAGGTTA
  GATAAACAAAGAGGGATCTTCCTATGGGAAATACCAAGGGAATCTTTGACCCAGGAACAGAGAATGTTC
  AGAGAAAACACTAACATTATCCGTAAAAGACCAAACTCAGAAGAGAAATGCCATAAATGTGAAGAATGT
  GGAAAGGGTTTTGTCCGCAAGGCCCATTTCATTCAACATCAAAGGGTCCATACTGGTGAGAAACCTTTT
  CAGTGCAATGAATGTGGGAAAAGTTTTAGTCGCAGTTCATTTGTTATTGAACATCAGAGAATTCACACT
  GGGGAAAGGCCCTATGAGTGTAATTACTGTGGAAAAACCTTTAGTGTGAGCTCAACCCTTATTAGACAT
  CAGAGAATCCACACTGGAGAAAGACCCTATCAGTGTAATCAGTGTAAACAGAGCTTCAGCCAGAGAAGG
  AGCCTTGTTAAACATCAAAGGATTCATACAGGTGAGAAACCCCATAAATGTAGTGACTGTGGGAAAGCC
  TTCAGTTGGAAATCACACCTTATTGAGCATCAAAGAACTCACACTGGTGAGAAACCTTATCACTGTACC
  AAATGTAAGAAGAGCTTTAGTCGAAATTCATTGCTTGTTGAGCATCAAAGAATTCACACTGGGGAAAGA
  CCCCATAAATGTGGTGAATGTGGGAAAGCCTTTCGATTAAGCACATACCTTATACAACACCAAAAAATT
  CACACTGGCGAGAAGCCTTTTCTTTGTATTGAGTGTGGAAAAAGTTTCAGTCGGAGCTCATTCCTTATT
  GAACATCAGAGGATCCATACTGGTGAAAGACCTTATCAGTGCAAAGAGTGTGGGAAAAGTTTCAGTCAG
  CTTTGCAACCTTACTCGTCATCAGAGAATTCACACAGGAGACAAGCCCCATAAATGTGAGGAATGTGGA
  AAAGCCTTTAGTAGAAGCTCAGGTCTTATTCAGCATCAGAGAATTCACACCAGGGAGAAGACTTATCCA
  TACAATGAAACTAAGGAAAGTTTTGATCCAAATTGCAGTCTTGTTATACAGCAGGAAGTCTACCCTAAG
  GAGAAATCTTATAAATGTGATGAATGTGGGAAAACTTTTAGTGTTAGTGCTCATCTTGTACAACATCAA
  AGAATCCACACTGGTGAAAAGCCCTATCTATGTACTGTCTGTGGGAAAAGCTTCAGCCGGAGCTCATTT
  CTTATTGAACATCAGAGAATCCACACTGGTGAGAGACCCTATCTGTGCAGACAGTGTGGAAAAAGCTTT
  AGTCAGCTTTGTAATCTTATTCGACATCAGGGTGTTCACACAGGTAATAAACCCCATAAATGTGATGAA
  TGTGGAAAGGCCTTTAGCCGGAACTCGGGTCTTATTCAGCATCAGAGAATACACACAGGAGAGAAACCT
  TATAAGTGTGAGAAGTGCGACAAAAGTTTCAGTCAACAGCGCAGTCTTGTCAACCATCAGAAGATCCAT
  GCAGAGGTGAAAACCCAAGAAACCCATGAATGTGACGCTTGTGGTGAAGCCTTTAATTGCCGTATTTCT
  CTTATTCAGCATCAGAAATTGCACACAGCATGGATGCAATAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001278232
• Insert Size: 1836 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001278232.1
• RefSeq Size: 3168 bp
• RefSeq ORF: 1836 bp
• Locus ID: 7743
• UniProt ID: O75820
• Cytogenetics: 9q31.1
• Protein Families: Transcription Factors
• MW: 71.3 kDa
• Gene Summary: Kruppel-like zinc finger proteins such as ZNF189 contain a conserved stretch of 7 amino acids that connects a variable number of DNA-binding zinc finger repeats of the cys(2)his(2) (C2H2) type (summarized by Odeberg et al., 1998 [PubMed 9653648]). Approximately 30% of human Kruppel-like zinc finger proteins contain an N-terminal Kruppel-associated box (KRAB) domain. The KRAB domain consists of approximately 75 amino acids that may be subdivided into an A box, which is present in every KRAB domain and is essential for transcriptional repression, and a B box, which is not always present.[supplied by OMIM, May 2010]
  Transcript Variant: This variant (4) uses an alternate in-frame splice junction at the 5' end of an exon compared to variant 1. The resulting isoform (4) has the same N- and C-termini but is shorter compared to isoform 1.