Cip4 (TRIP10) (NM_001288963) Human Untagged Clone

CAT#: SC336914

TRIP10 (untagged) - Human thyroid hormone receptor interactor 10 (TRIP10), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Cip4
• Synonyms: CIP4; HSTP; STOT; STP; TRIP-10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC336914 representing NM_001288963.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGATTGGGGCACTGAGCTGTGGGATCAGTTCGAGGTGCTCGAGCGCCACACGCAGTGGGGGCTGGAC
  CTGTTGGACAGATATGTAAAGTTCGTGAAAGAACGCACCGAAGTGGAACAGGCTTACGCCAAACAACTG
  CGGAGCCTGGTGAAAAAATATCTGCCCAAGAGACCTGCCAAGGATGATCCTGAGTCCAAATTCAGCCAG
  CAACAGTCCTTCGTACAGATTCTCCAGGAGGTGAATGACTTTGCAGGCCAGCGGGAGCTGGTGGCTGAG
  AACCTCAGTGTCCGTGTATGTCTTGAGCTGACCAAGTACTCACAAGAGATGAAACAGGAGAGGAAGATG
  CACTTCCAAGAAGGGCGGCGGGCCCAGCAGCAGCTGGAAAATGGCTTTAAACAGCTGGAGAATAGTAAG
  CGTAAATTTGAGCGGGACTGCCGGGAGGCAGAGAAGGCAGCCCAGACTGCTGAACGGCTAGACCAGGAT
  ATCAACGCCACCAAGGCTGATGTGGAGAAGGCCAAGCAGCAAGCCCACCTTCGGAGTCACATGGCCGAA
  GAAAGCAAAAACGAATATGCGGCTCAACTGCAGCGCTTCAACCGAGACCAAGCCCACTTCTATTTTTCA
  CAGATGCCCCAGATATTCGATAAGCTCCAAGACATGGATGAACGCAGGGCCACCCGCCTGGGTGCCGGG
  TATGGGCTCCTGTCGGAGGCCGAGCTGGAGGTGGTGCCCATAATAGCCAAGTGCTTGGAGGGCATGAAG
  GTGGCTGCAAATGCTGTGGATCCCAAGAACGACTCCCACGTCCTTATAGAGCTGCACAAGTCAGGTTTT
  GCCCGCCCGGGCGACGTGGAATTCGAGGACTTCAGCCAGCCCATGAACCGTGCACCCTCCGACAGCAGT
  CTGGGCACCCCCTCGGATGGACGGCCTGAACTCCGAGGCCCGGGTCGCAGCCGCACCAAGCGCTGGCCT
  TTTGGCAAGAAGAACAAGACAGTGGTGACCGAGGATTTTAGCCACTTGCCCCCAGAGCAGCAGCGAAAA
  CGGCTTCAACAGCAGTTGGAAGAACGCAGTCGTGAACTTCAGAAGGAGGTTGACCAGAGGGAAGCCCTA
  AAGAAAATGAAGGATGTCTATGAGAAGACACCTCAGATGGGGGACCCCGCCAGCTTGGAGCCCCAGATC
  GCTGAAACCCTGAGCAACATTGAACGGCTGAAATTGGAAGTGCAGAAGTATGAGGCGTGGCTGGCAGAA
  GCTGAAAGTCGAGTCCTTAGCAACCGGGGAGACAGCCTGAGCCGGCACGCCCGGCCTCCCGACCCCCCC
  GCTAGCGCCCCGCCAGACAGCAGCAGCAACAGCGCATCACAGGACACCAAGGAGAGCTCTGAAGAGCCT
  CCCTCAGAAGAGAGCCAGGACACCCCCATTTACACGGAGTTTGATGAGGATTTCGAGGAGGAACCCACA
  TCCCCCATAGGTCACTGTGTGGCCATCTACCACTTTGAAGATCTGGGCCCACCCCCACCCCCATCACAG
  GGTCCAGCGAGGGCACTATCTCTATGGCCGAGGGTGAAGACCTCAGTCTTATGGAAGAAGACAAAGGGG
  ACGGCTGGACCCGGGTCAGGCGGAAAGAGGGAGGCGAGGGCTACGTGCCCACCTCCTACCTCCGAGTCA
  CGCTCAATTGAACCCTGCCAGAGACGGGAAGAGGGGGGCTGTCGGCTGCTGCTTCTGGGCCACGGGGAG
  CCCCAGGACCTATGCACTTTATTTCTGACCCCGTGGCTTCGGCTGAGACCTGTGTAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001288963
• Insert Size: 1782 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001288963.1
• RefSeq Size: 2062 bp
• RefSeq ORF: 1782 bp
• Locus ID: 9322
• UniProt ID: Q15642
• Cytogenetics: 19p13.3
• Protein Families: Druggable Genome
• Protein Pathways: Insulin signaling pathway
• MW: 67.7 kDa
• Gene Summary: Required for translocation of GLUT4 to the plasma membrane in response to insulin signaling (By similarity). Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during endocytosis. Binds to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promotes membrane invagination and the formation of tubules. Also promotes CDC42-induced actin polymerization by recruiting WASL/N-WASP which in turn activates the Arp2/3 complex. Actin polymerization may promote the fission of membrane tubules to form endocytic vesicles. Required for the formation of podosomes, actin-rich adhesion structures specific to monocyte-derived cells. May be required for the lysosomal retention of FASLG/FASL.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) lacks an in-frame exon in the central coding region, and uses an alternate splice site in the 3' coding region, which leads to a frameshift, compared to variant 1. The encoded isoform (3) lacks an internal segment and has a longer and distinct C-terminus, compared to isoform 1.