CDH18 (NM_001291957) Human Untagged Clone

CAT#: SC336848

CDH18 (untagged) - Human cadherin 18, type 2 (CDH18), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CDH18
• Synonyms: CDH14; CDH14L; CDH24
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC336848 representing NM_001291957.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAAAATTACTAGCACATCTTGCATCTGTCCAGTCCTAGTGTGTCTCTGTTTTGTGCAGAGGTGTTAT
  GGAACTGCTCACCACAGCTCCATCAAGGTGATGAGAAACCAAACCAAACACATTGAAGGTGAAACCGAA
  GTCCATCATCGTCCCAAAAGGGGATGGGTATGGAATCAGTTCTTTGTTTTAGAAGAACATATGGGACCA
  GATCCTCAGTATGTTGGAAAGCTGCACTCCAATTCTGACAAAGGTGATGGATCTGTCAAGTACATCCTT
  ACTGGAGAGGGTGCTGGGACTATATTTATCATTGACGATACCACGGGTGATATCCACTCAACAAAAAGC
  CTAGACAGAGAGCAGAAGACCCACTATGTGCTTCATGCTCAAGCTATTGATAGACGTACAAACAAACCT
  CTTGAGCCTGAATCCGAGTTCATCATCAAAGTGCAAGACATCAATGACAACGCTCCAAAATTCACAGAT
  GGACCATACATTGTTACTGTGCCTGAAATGTCAGATATGGGTACCTCTGTTCTACAGGTGACAGCTACT
  GATGCAGATGACCCTACCTATGGAAACAGCGCTCGGGTGGTTTACAGCATTCTCCAAGGACAACCCTAC
  TTCTCCGTCGACCCTAAAACAGGAGTTATTAGAACGGCCTTACATAACATGGACAGAGAAGCCAGAGAA
  CATTACTCCGTAGTCATTCAAGCCAAAGACATGGCTGGGCAAGTTGGAGGGCTTTCAGGATCTACAACA
  GTCAACATCACCTTAACCGATGTCAATGACAACCCACCACGCTTTCCTCAAAAACACTATCAGCTATAT
  GTTCCTGAGTCAGCTCAAGTTGGTTCAGCTGTTGGGAAAATCAAGGCAAATGATGCTGACACTGGCTCA
  AATGCTGACATGACCTACTCCATCATAAATGGTGATGGCATGGGAATATTCTCAATCTCCACTGACAAA
  GAGACCAGAGAAGGAATCCTTTCTTTAAAGAAGCCACTGAACTATGAGAAAAAGAAGTCATATACCCTC
  AACATAGAAGGAGCAAATACACATCTTGATTTTCGCTTTTCTCACTTGGGTCCTTTTAAAGATGCTACT
  ATGCTGAAGATCATTGTTGGGGATGTAGATGAACCACCACTATTTTCCATGCCTTCCTACCTCATGGAA
  GTCTACGAAAATGCCAAGATTGGGACCGTCGTTGGTACAGTTTTGGCACAAGATCCTGACAGTACTAAC
  AGCTTAGTAAGATACTTCATCAACTACAATGTTGAAGACGACAGATTTTTCAACATTGATGCCAATACT
  GGGACCATTAGGACTACAAAGGTTCTCGACAGAGAAGAAACTCCATGGTACAACATCACAGTCACTGCT
  TCAGAAATTGATAATCCTGATTTGCTGAGCCATGTCACAGTGGGTATTAGAGTTCTGGATGTCAATGAC
  AATCCACCCGAACTTGCCAGGGAATATGATATTATTGTATGTGAAAATTCTAAGCCTGGCCAGGTTATT
  CATACCATCAGTGCCACTGATAAAGATGATTTTGCCAATGGACCAAGGTTTAACTTCTTTCTTGATGAA
  CGCCTGCCTGTAAATCCAAACTTCACTCTGAAGGACAATGAAGCAGCAGCAGCACCCTCACCATCAGGG
  TTTGTGCATGCGAGAGAGATGGGCGTGTGCGGACCTGCCATGCAGAAGCCTTCCTGTCCTCGGCTGGTT
  TGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001291957
• Insert Size: 1728 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291957.1
• RefSeq Size: 2974 bp
• RefSeq ORF: 1728 bp
• Locus ID: 1016
• Cytogenetics: 5p14.3
• Protein Families: Transmembrane
• MW: 63.7 kDa
• Gene Summary: This gene encodes a type II classical cadherin from the cadherin superfamily of integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. This particular cadherin is expressed specifically in the central nervous system and is putatively involved in synaptic adhesion, axon outgrowth and guidance. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014]
  Transcript Variant: This variant (4) differs in the 5' UTR and has an alternate splice site in the 3' coding region, which results in a frameshift, compared to variant 1. It encodes isoform 3, which has a shorter and distinct C-terminus, compared to isoform 1. Variant 4 and 8 encode the same protein (isoform 3). This isoform (3) may undergo proteolytic processing similar to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.