LILRB2 (NM_001278405) Human Untagged Clone

CAT#: SC336533

LILRB2 (untagged) - Human leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 2 (LILRB2), transcript variant 5

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LILRB2
• Synonyms: CD85D; ILT-4; ILT4; LIR-2; LIR2; MIR-10; MIR10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC336533 representing NM_001278405.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGACCCCCATCGTCACAGTCCTGATCTGTCTCGGGCTGAGTCTGGGCCCCAGGACCCGCGTGCAGACA
  GGGACCATCCCCAAGCCCACCCTGTGGGCTGAGCCAGACTCTGTGATCACCCAGGGGAGTCCCGTCACC
  CTCAGTTGTCAGGGGAGCCTTGAAGCCCAGGAGTACCGTCTATATAGGGAGAAAAAATCAGCATCTTGG
  ATTACACGGATACGACCAGAGCTTGTGAAGAACGGCCAGTTCCACATCCCATCCATCACCTGGGAACAC
  ACAGGGCGATATGGCTGTCAGTATTACAGCCGCGCTCGGTGGTCTGAGCTCAGTGACCCCCTGGTGCTG
  GTGATGACAGGAGCCTACCCAAAACCCACCCTCTCAGCCCAGCCCAGCCCTGTGGTGACCTCAGGAGGA
  AGGGTGACCCTCCAGTGTGAGTCACAGGTGGCATTTGGCGGCTTCATTCTGTGTAAGGAAGGAGAAGAT
  GAACACCCACAATGCCTGAACTCCCAGCCCCATGCCCGTGGGTCGTCCCGCGCCATCTTCTCCGTGGGC
  CCCGTGAGCCCGAATCGCAGGTGGTCGCACAGGTGCTATGGTTATGACTTGAACTCTCCCTATGTGTGG
  TCTTCACCCAGTGATCTCCTGGAGCTCCTGGTCCCAGGTGTTTCTAAGAAGCCATCACTCTCAGTGCAG
  CCGGGTCCTGTCATGGCCCCTGGGGAAAGCCTGACCCTCCAGTGTGTCTCTGATGTCGGCTATGACAGA
  TTTGTTCTGTACAAGGAGGGGGAACGTGACCTTCGCCAGCTCCCTGGCCGGCAGCCCCAGGCTGGGCTC
  TCCCAGGCCAACTTCACCCTGGGCCCTGTGAGCCGCTCCTACGGGGGCCAGTACAGATGCTACGGTGCA
  CACAACCTCTCCTCTGAGTGCTCGGCCCCCAGCGACCCCCTGGACATCCTGATCACAGGACAGATCCGT
  GGCACACCCTTCATCTCAGTGCAGCCAGGCCCCACAGTGGCCTCAGGAGAGAACGTGACCCTGCTGTGT
  CAGTCATGGCGGCAGTTCCACACTTTCCTTCTGACCAAGGCGGGAGCAGCTGATGCCCCACTCCGTCTA
  AGATCAATACACGAATATCCTAAGTACCAGGCTGAATTCCCCATGAGTCCTGTGACCTCAGCCCACGCG
  GGGACCTACAGGTGCTACGGCTCACTCAACTCCGACCCCTACCTGCTGTCTCACCCCAGTGAGCCCCTG
  GAGCTCGTGGTCTCAGGACCCTCCATGGGTTCCAGCCCCCCACCCACCGGTCCCATCTCCACACCTGCA
  GGCCCTGAGGACCAGCCCCTCACCCCCACTGGGTCGGATCCCCAAAGTGGTCTGGGAAGGCACCTGGGG
  GTTGTGATCGGCATCTTGGTGGCCGTCGTCCTACTGCTCCTCCTCCTCCTCCTCCTCTTCCTCATCCTC
  CGACATCGACGTCAGGGCAAACACTGGACATCGAGTCCAGCCCAGCTGCCGACGCCCAGGAAGAAAACC
  TCTATGCTGCCGTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001278405
• Insert Size: 1533 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001278405.2
• RefSeq Size: 2646 bp
• RefSeq ORF: 1533 bp
• Locus ID: 10288
• Cytogenetics: 19q13.42
• Protein Families: Transmembrane
• MW: 55.5 kDa
• Gene Summary: This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (5) has a shorter 5' UTR, and lacks an internal exon which results in a frameshift and an early stop codon, compared to variant 1. The encoded isoform (4) has a shorter and distinct C-terminus, compared to isoform 1.