LONRF3 (NM_001289109) Human Untagged Clone

CAT#: SC336491

LONRF3 (untagged) - Human LON peptidase N-terminal domain and ring finger 3 (LONRF3), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LONRF3
• Synonyms: RNF127
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC336491 representing NM_001289109.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGGTTTTAAGGCACATTTCAGAAAAGCCCAAGCCTTAGCCACCCTAGGCAAGGTGGAGGAGGCACTA
  AGGGAGTTTCTCTACTGTGTATCCCTTGATGGAAAGAACAAGAGAGCAAGATGTGAAGCCCAAAGACTT
  CTCCTTAGTTTCTTTTCACCATCAGTCCCGGGGGATTCTCAGGAACATTCTCCCGATATCCTGAAGCTG
  TTGGCACCTCACCCTAGATTAAAGGAAAACGTAGAGTCAATGACTACTGAAGTTACCAGCCATAATCTA
  CCAAGGTTGTTACAGGACAATCTGGAGCTCCCACATTGTTCTAGTCAGGAGGAAGCAGCAGCCAGGGGA
  GATGGCAGCAGTCTGATGGACCCAGCTAAAGTGAAGGGGGATGGTCAGCAGCACCACATGAAAGACCAG
  GAAGAAGAGGAGGAGAAGTGGGATGCTACCTCTCCAAAAGCTGCTTCCAGCAAGACTGGAAAATGCCAG
  GAAAAGAAAAGGAAACATTGCCAGATTGAATCCCAAGAAGAAACGGGGATGCCTAATAAAGCCTCCAAG
  CAAGATCCTCCCACTGATCAGGGGGACAAACCTGCTCTCAGTTTACCACTTGCATCTTTCGACGCATCT
  GACCTTGAATGCGCTCTATGTATGAGATTATTCTATGAGCCAGTCACAACACCTTGCGGGCATACTTTT
  TGCTTAAAATGCCTAGAAAGATGCCTAGATCACAACGCAAAGTGTCCATTGTGCAAAGACGGTCTTTCA
  CAGTGCTTGGCATCAAGAAAATACAGCAAAAATGTAATAATGGAGGAGCTCATAGCTAAATTCCTTCCA
  GAAGAACTGAAGGAACGAAGGAAGCTTTATGAAGAGGAAATGGAAGAACTTTCTAACCTTAATAAGAAT
  GTGCCTATTTTCGTGTGTACTATGGCCTATCCCACCGTTCCTTGTCCCCTGCACATCTTTGAGCCTTGT
  TACCGCCTGATGATTCGTAGATGCATTGAGACAGGCACGAGACAGTTTGGCATGTGCCTTGGAGATCCT
  GTCAAAGGGTTTGCAGAATATGGCTGCATCCTAGAGATCAGAAATGTTCAATTCTTTGCTGATGGCCGC
  TCAGTGGTTGACAGCATAGGCAAGAGGCGCTTCAGGGTGCTCCATCAGAGCCAGCGGGATGGCTACAAC
  ACAGCCGACATTGAATACATTGAAGACCAAAAGGTTCAGGGAGAGGATTGTGCTGAGCTCATGGGATTA
  CATAACTGTGTCTATCAGCAAGCATCATTGTGGTTTCATTCGCTCAAATTATCCCTAAAGAATCGGATA
  CTCAATCACTTTGGTCCCATGCCGGAGAAAGACGCCGATCCTCAGATGAACCCGAATGGCCCAGCCTGG
  TGCTGGTGGATGTTAGCAGTTCTTCCCTTGGAAAGCCGAGCTCAGCTCCCCTTCCTAGCAATGAGGTCC
  TTAAAGGACAGACTGAATGGTATTCGACGAGTCCTGGCCTTCATATCCCGAAACCAAAACTAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289109
• Insert Size: 1512 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289109.1
• RefSeq Size: 2898 bp
• RefSeq ORF: 1512 bp
• Locus ID: 79836
• Cytogenetics: Xq24
• Protein Families: Druggable Genome, Protease
• MW: 57.1 kDa
• Gene Summary: The protein encoded by this gene contains a RING finger domain, a motif present in a variety of functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions. Multiple alternatively spliced transcript variants have been suggested, but their full length natures are not clear. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (3) differs in the 5' UTR and coding region, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. This results in a protein (isoform 3) which has a shorter N-terminus, compared to isoform 1.