SON (NM_001291412) Human Untagged Clone

CAT#: SC336215

SON (untagged) - Human SON DNA binding protein (SON), transcript variant h

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SON
• Synonyms: BASS1; C21orf50; DBP-5; NREBP; SON3; TOKIMS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC336215 representing NM_001291412.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCGACCAACATCGAGCAGATTTTTAGGTCTTTCGTGGTCAGTAAATTCCGGGAAATTCAACAGGAG
  CTTTCCAGTGGAAGGAATGAAGGCCAGCTGAATGGTGAAACAAATACACCCATTGAAGGAAACCAGGCG
  GGTGATGCAGCTGCCTCTGCCAGGAGTCTACCAAATGAAGAAATAGTGCAGAAGATAGAGGAAGTACTT
  TCTGGGGTCTTAGATACAGAACTACGATATAAGCCAGATAAGGCTCAATTACTTGAAATAGCCAAAGCT
  AATGCAGCTGCCATGTGTGCTAAGGCTGGTGTCCCTTTACCACCAAACCTAAAGCCTGCACCTCCACCT
  ACTATAGAAGAGAAAGTTGCTAAAAAGTCAGGAGGAGCTACTATAGAAGAACTAACTGAGAAATGTAAA
  CAGATCGCACAGAGTAAAGAAGATGATGATGTAATAGTGAATAAACCTCATGTTTCGGATGAAGAGGAA
  GAAGAACCTCCTTTTTATCATCATCCCTTTAAACTCAGTGAACCCAAACCTATTTTTTTCAATCTGAAT
  ATTGCTGCAGCAAAACCAACTCCACCAAAAAGCCAGGTAACATTAACAAAAGAATTCCCTGTATCATCT
  GGATCTCAACATCGGAAAAAAGAAGCGGATAGTGTTTATGGAGAATGGGTTCCTGTGGAGAAAAATGGT
  GAAGAAAACAAAGATGATGATAATGTTTTCAGCAGCAATTTGCCCTCAGAGCCTGTGGACATCTCTACA
  GCAATGAGTGAACGGGCACTTGCTCAGAAAAGACTCAGTGAGAATGCATTTGATCTTGAAGCCATGAGC
  ATGTTAAATAGAGCTCAGGAAAGGATTGATGCCTGGGCTCAGCTGAACTCTATTCCTGGCCAGTTCACA
  GGAAGTACAGGAGTACAGGTTTTGACACAAGAACAGTTGGCCAATACTGGTGCCCAAGCCTGGATTAAA
  AAGGATCAGTTCTTAAGAGCAGCCCCGGTAACTGGAGGAATGGGAGCCGTTTTGATGAGAAAAATGGGC
  TGGAGAGAAGGAGAAGGATTAGGAAAAAACAAAGAAGGCAATAAGGAACCCATCCTAGTTGATTTTAAG
  ACAGACCGAAAAGGTCTTGTTGCAGTAGGAGAAAGAGCACAAAAGAGGTCTGGGAACTTCTCTGCTGCA
  ATGAAAGATCTGTCAGGCAAACATCCTGTGTCTGCTTTGATGGAGATCTGTAATAAAAGAAGGTGGCAA
  CCACCTGAATTTCTATTGGTCCATGATAGTGGCCCTGATCATCGCAAACATTTTCTCTTTAGGGTATTG
  AGAAATGGAGCCCTTACCAGACCCAATTGTATGTTTTTCTTGAATAGGTATTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001291412
• Insert Size: 1365 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_001291412.1
• RefSeq Size: 2524 bp
• RefSeq ORF: 1365 bp
• Locus ID: 6651
• UniProt ID: P18583
• Cytogenetics: 21q22.11
• MW: 50.3 kDa
• Gene Summary: This gene encodes a protein that contains multiple simple repeats. The encoded protein binds RNA and promotes pre-mRNA splicing, particularly of transcripts with poor splice sites. The protein also recognizes a specific DNA sequence found in the human hepatitis B virus (HBV) and represses HBV core promoter activity. There is a pseudogene for this gene on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
  Transcript Variant: This variant (h) lacks an internal in-frame exon, compared to variant f. The encoded isoform (H) is shorter than isoform F.