BECN2 (NM_001290693) Human Untagged Clone

CAT#: SC336082

BECN1P1 (untagged) - Human beclin 1, autophagy related, pseudogene 1 (BECN1P1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BECN2
• Synonyms: BECN1L1; BECN1P1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC336082 representing NM_001290693.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTCTTCCATCCGCTTCCTGTGCCAGCGCTGCCACCAGGCCCTGAAGCTGAGCGGCTCCTCGGAGTCT
  AGGAGCCTCCCTGCAGCCCCGGCGCCCACCTCTGGGCAGGCTGAGCCCGGAGACACCCGGGAGCCCGGC
  GTCACCACCAGGGAGGTGACAGACGCTGAGGAGCAACAGGACGGTGCCTCTAGCAGATCCCCTCCAGGC
  GATGGCAGTGTGTCCAAGGGCCATGCCAACATCTTCACCCTGCTGGGGGAGCTTGGCGCCATGCACATG
  CTCAGTAGCATCCAGAAGGCAGCTGGTGACATTTTTGACATAGTCTCTGGCCAAGCAGTTGTGGACCAT
  CCCCTGTGTGAAGAATGCACCGACAGTCTTTTAGAGCAGCTGGACATCCAGCTCGCTCTCACAGAAGCT
  GACAGTCAGAACTACCAACGCTGCCTGGAGACCGGGGAGCTGGCGACCAGCGAGGACGAGGCGGCGGCG
  CTGCGGGCGGAGCTGCGGGACCTGGAGCTGGAGGAGGCCAGGCTGGTGCAGGAGCTGGAGGATGTGGAC
  AGGAACAATGCAAGAGCAGCGGCGGATCTCCAGGCAGCCCAGGCAGAGGCTGCGGAGCTGGACCAGCAG
  GAGAGGCAGCACTACAGGGACTACAGTGCCTTGAAGCGGCAGCAGCTGGAACTGCTTGATCAGCTGGGG
  AACGTGGAGAACCAGCTGCAGTATGCCAGGGTCCAGAGGGACCGGCTGAAGGAAATCAACTGTTTCACC
  GCCACGTTTGAGATCTGGGTGGAGGGCCCCTTGGGCGTCATCAATAACTTCAGGTTGGGCCGCCTCCCC
  ACTGTCCGTGTGGGCTGGAATGAGATTAACACTGCCTGGGGACAGGCGGCCTTGCTGCTCCTTACCCTG
  GCCAATACAATTGGACTGCAGTTTCAGAGGTATCGACTCATCCCCTGCGGAAACCATTCGTATCTGAAG
  TCTTTAACAGATGACCGCACTGAGCTGCCGTTGTTCTGTTATGGGGGGCAGGATGTTTTCCTCAATAAC
  AAGTATGACCGCGCGATGGTGGCCTTCCTGGACTGCATGCAGCAGTTCAAGGAAGAGGCTGAGAAGGGT
  GAGCTGGGCCTCTCTCTGCCCTACGGGATCCAGGTGGAGACAGGCCTGATGGAGGACGTTGGCGGCCGA
  GGGGAATGCTATTCCATCAGAACCCATCTGAACACGCAGGAGCTGTGGACAAAGGCACTCAAGTTCATG
  CTTATAAATTTCAAGTGGAGTCTCATCTGGGTTGCCTCAAGGTATCAAAAGTAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290693
• Insert Size: 1296 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290693.1
• RefSeq Size: 1296 bp
• RefSeq ORF: 1296 bp
• Locus ID: 441925
• UniProt ID: A8MW95
• Cytogenetics: 1q43
• Protein Pathways: Regulation of autophagy
• MW: 48.2 kDa
• Gene Summary: Involved in 2 distinct lysosomal degradation pathways: acts as a regulator of autophagy and as a regulator of G-protein coupled receptors turnover. Regulates degradation in lysosomes of a variety of G-protein coupled receptors via its interaction with GPRASP1/GASP1.[UniProtKB/Swiss-Prot Function]