TBCE (NM_001287802) Human Untagged Clone

CAT#: SC335985

TBCE (untagged) - Human tubulin folding cofactor E (TBCE), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TBCE
• Synonyms: HRD; KCS; KCS1; pac2; PEAMO
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC335985 representing NM_001287802.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTATCCATGTGAACTCTGTTTTTCATGCAGTCAGCTGAGCAAGTTGCAAGAAGTTTCTCTGAGGAAC
  TGTGCAGTAAGTTGTGCTGGTGAAAAAGGAGGAGTTGCTGAAGCATGTCCTAATATCAGAAAGGTAGAT
  TTGTCAAAAAACCTGTTGTCATCATGGGATGAAGTGATACACATTGCTGATCAGCTCAGACACCTGGAA
  GTCCTTAATGTCAGTGAAAATAAACTAAAATTTCCCTCCGGTTCAGTATTAACTGGAACGCTTTCTGTA
  CTGAAGGTTTTAGTCCTCAATCAAACAGGAATAACGTGGGCTGAGGTGCTGCGGTGTGTCGCGGGGTGC
  CCAGGCCTGGAGGAACTCTACCTTGAGTCTAACAACATTTTCATTTCCGAAAGGCCAACAGATGTTCTC
  CAGACAGTCAAGTTATTAGATCTTTCCTCTAATCAATTAATTGATGAAAATCAGCTGTATCTGATAGCC
  CACCTGCCCAGGTTAGAACAATTAATCCTCTCTGACACTGGAATTTCTTCTCTACATTTTCCGGATGCT
  GGAATTGGGTGCAAAACGTCCATGTTCCCATCCTTGAAGTACCTGGTAGTAAACGACAATCAGATATCA
  CAATGGTCGTTTTTCAATGAGCTAGAGAAGTTACCAAGTCTACGGGCTTTGTCCTGCCTAAGAAACCCC
  CTGACCAAAGAGGACAAAGAAGCAGAGACGGCGCGACTACTCATTATCGCCAGCATTGGCCAGCTGAAG
  ACGCTGAACAAATGTGAGATTCTCCCCGAGGAGAGGCGGAGAGCTGAGCTTGACTACCGAAAAGCTTTT
  GGAAATGAGTGGAAACAGGCTGGTGGACATAAGGATCCGGAAAAAAACAGACTCAGCGAAGAATTCCTC
  ACAGCCCATCCCAGATACCAGTTCCTCTGCCTGAAATATGGTGCACCTGAAGATTGGGAACTCAAAACA
  CAGCAACCACTTATGCTGAAAAACCAGCTACTAACACTGAAGATAAAATACCCTCATCAACTTGATCAG
  AAAGTCCTGGAGAAACAACTGCCGGGCTCCATGACAATTCAAAAGGTGAAGGGATTGCTGTCACGTCTT
  CTCAAAGTTCCTGTGTCAGACCTTCTGTTGTCCTATGAAAGTCCCAAAAAGCCGGGCAGAGAAATCGAG
  CTGGAAAATGACCTAAAGTCATTACAGTTTTATTCTGTGGAAAATGGAGATTGTCTATTAGTGCGATGG
  TGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001287802
• Insert Size: 1245 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001287802.1
• RefSeq Size: 1948 bp
• RefSeq ORF: 1245 bp
• Locus ID: 6905
• UniProt ID: Q15813
• Cytogenetics: 1q42.3
• Protein Families: Druggable Genome
• MW: 46.9 kDa
• Gene Summary: Cofactor E is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin intermediates in a quasi-native confirmation. Cofactor E binds to the cofactor D/beta-tubulin complex; interaction with cofactor C then causes the release of beta-tubulin polypeptides that are committed to the native state. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (4) differs in its 5' UTR, initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (c) has a shorter and distinct N-terminus, compared to isoform a.