SLC35A2 (NM_001282650) Human Untagged Clone

CAT#: SC335916

SLC35A2 (untagged) - Human solute carrier family 35 (UDP-galactose transporter), member A2 (SLC35A2), transcript variant 7

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SLC35A2
• Synonyms: CDG2M; CDGX; UDP-Gal-Tr; UGALT; UGAT; UGT; UGT1; UGT2; UGTL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC335916 representing NM_001282650.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCAGCGGTTGGGGCTGGTGGTTCCACCGCGGCGCCCGGGCCAGGGGCGGTTTCCGCGGGTGCATTG
  GAGCCGGGGACCGCCAGTGCGGGTGAGACAGTCTGCCCGAGCAGTCGCATGGGAGGCGGAGCTCACAGG
  CGCCTGAAGTACATATCCCTAGCTGTGCTGGTGGTCCAGAATGCCTCCCTCATCCTCAGCATCCGCTAC
  GCCCGCACGTTGCCAGGGGACCGCTTCTTTGCCACCACTGCTGTGGTCATGGCGGAAGTGCTCAAAGGT
  CTCACCTGCCTGCTGCTGCTCTTCGCACAGAAGAGGGGTAACGTGAAGCACCTGGTTCTCTTCCTCCAT
  GAGGCTGTCCTGGTGCAGTATGTGGACACGCTCAAGCTCGCAGTGCCCTCTCTCATCTACACCTTGCAG
  AATAACCTCCAGTATGTTGCCATCTCTAACCTACCAGCTGCCACTTTCCAGGTGACATACCAGCTGAAG
  ATCCTGACCACAGCGCTGTTCTCCGTGCTCATGCTGAATCGCAGCCTTTCCCGGCTGCAGTGGGCCTCC
  CTGCTGCTCCTCTTCACTGGCGTCGCCATTGTCCAGGCACAGCAAGCCGGTGGGGGAGGCCCACGGCCA
  CTGGATCAGAACCCTGGGGCAGGCCTGGCAGCCGTCGTGGCCTCCTGTCTCTCCTCCGGCTTCGCAGGT
  GTCTACTTTGAGAAGATCCTCAAAGGCAGCTCAGGCTCCGTGTGGCTGCGCAACCTGCAACTGGGCCTC
  TTCGGCACAGCACTGGGCCTGGTGGGGCTCTGGTGGGCTGAGGGTACCGCCGTGGCCACCCGTGGTTTC
  TTTTTTGGGTACACACCTGCTGTCTGGGGCGTGGTGCTCAACCAGGCCTTCGGCGGGCTACTGGTGGCT
  GTGGTTGTCAAGTACGCTGACAATATCCTCAAGGGCTTTGCCACCTCCCTGTCCATTGTGCTGTCCACT
  GTTGCCTCCATTCGCCTCTTTGGCTTCCACGTGGACCCATTATTTGCCCTTGGCGCTGGACTCGTCATT
  GGTGCTGTCTACCTCTACAGCCTTCCCCGAGGTGCAGCCAAAGCCATAGCCTCTGCCTCTGCCTCCGCC
  TCCGGGCCCTGCGTTCACCAGCAGCCTCCCGGGCAGCCACCACCACCGCAGCTGTCTTCCCACCGTGGA
  GACCTCATCACGGAGCCCTTTCTGCCAAAGTCAGTGCTGGTGAAGTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001282650
• Insert Size: 1221 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001282650.1
• RefSeq Size: 2658 bp
• RefSeq ORF: 1221 bp
• Locus ID: 7355
• UniProt ID: P78381
• Cytogenetics: Xp11.23
• Protein Families: Transmembrane
• MW: 42.2 kDa
• Gene Summary: This gene encodes a member of the nucleotide-sugar transporter family. The encoded protein is a multi-pass membrane protein. It transports UDP-galactose from the cytosol into Golgi vesicles, where it serves as a glycosyl donor for the generation of glycans. Mutations in this gene cause congenital disorder of glycosylation type IIm (CDG2M). Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Oct 2014]
  Transcript Variant: This variant (7) uses an alternate in-frame splice site in the 5' coding region and differs in the 3' exon structure, compared to variant 1. The encoded isoform (g) is longer and has a distinct C-terminus, compared to isoform a.