Transmembrane Protein 175 (TMEM175) (NM_001297426) Human Untagged Clone

CAT#: SC335800

TMEM175 (untagged) - Human transmembrane protein 175 (TMEM175), transcript variant 5

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Transmembrane Protein 175
• Synonyms: hTMEM175
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC335800 representing NM_001297426.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGATGACCATCACCTTCCTGCCTTACACGTTTTCGTTAATGGTGACCTTCCCTGATGTGCCTCTGGGC
  ATCTTCTTGTTCTGTGTGTGTGTGATCGCCATTGGGGTCGTGCAGGCACTGATTGTGGGGTACGCATTC
  CACTTCCCGCACCTGCTGAGCCCGCAGATCCAGCGCTCTGCCCACAGGGCTCTGTACCGACGACACGTC
  CTGGGCATCGTCCTCCAAGGCCCGGCCCTGTGCTTTGCAGCGGCCATCTTCTCTCTCTTCTTTGTCCCC
  TTGTCTTACCTGCTGATGGTGACTGTCATCCTCCTCCCCTATGTCAGCAAGGTCACCGGCTGGTGCAGA
  GACAGGCTCCTGGGCCACAGGGAGCCCTCGGCTCACCCAGTGGAAGTCTTCTCGTTTGACCTCCACGAG
  CCACTCAGCAAGGAGCGCGTGGAAGCCTTCAGCGACGGAGTCTACGCCATCGTGGCCACGCTTCTCATC
  CTGGACATCTGCGAAGACAACGTCCCGGACCCCAAGGATGTGAAGGAGAGGTTCAGCGGCAGCCTCGTG
  GCCGCCCTGAGTGCGACCGGGCCGCGCTTCCTGGCGTACTTCGGCTCCTTCGCCACAGTGGGACTGCTG
  TGGTTCGCCCACCACTCACTCTTCCTGCATGTGCGCAAGGCCACGCGGGCCATGGGGCTGCTGAACACG
  CTCTCGCTGGCCTTCGTGGGTGGCCTCCCACTAGCCTACCAGCAGACCTCGGCCTTCGCCCGGCAGCCC
  CGCGATGAGCTGGAGCGCGTGCGTGTCAGCTGCACCATCATCTTCCTGGCCAGCATCTTCCAGCTGGCC
  ATGTGGACCACGGCGCTGCTGCACCAGGCGGAGACGCTGCAGCCCTCGGTGTGGTTTGGCGGCCGGGAG
  CATGTGCTCATGTTCGCCAAGCTGGCGCTGTACCCCTGTGCCAGCCTGCTGGCCTTCGCCTCCACCTGC
  CTGCTGAGCAGGTTCAGTGTGGGCATCTTCCACCTCATGCAGATCGCCGTGCCCTGCGCCTTCCTGTTG
  CTGCGCCTGCTCGTGGGCCTGGCCCTGGCCACCCTGCGGGTCCTGCGGGGCCTCGCCCGGCCCGAACAC
  CCCCCGCCAGCCCCCACGGGCCAGGACGACCCACAGTCCCAGCTCCTCCCTGCCCCCTGCTAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001297426
• Insert Size: 1167 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001297426.1
• RefSeq Size: 2125 bp
• RefSeq ORF: 1167 bp
• Locus ID: 84286
• UniProt ID: Q9BSA9
• Cytogenetics: 4p16.3
• Protein Families: Transmembrane
• MW: 42.8 kDa
• Gene Summary: Organelle-specific potassium channel specifically responsible for potassium conductance in endosomes and lysosomes. Forms a potassium-permeable leak-like channel, which regulates lumenal pH stability and is required for autophagosome-lysosome fusion. Constitutes the major lysosomal potassium channel.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (5) differs in its 5' UTR and uses a downstream start codon, compared to variant 1. The encoded isoform (3) has a shorter N-terminus, compared to isoform 1. Variants 5, 6, and 7 encode the same isoform.