VPS36 (NM_001282168) Human Untagged Clone

CAT#: SC335737

VPS36 (untagged) - Human vacuolar protein sorting 36 homolog (S. cerevisiae) (VPS36), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: VPS36
• Synonyms: C13orf9; CGI-145; EAP45
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC335737 representing NM_001282168.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGACCGCTTCGTTTGGACCAGCGGCCTCCTGGAGATCAACGAGACCCTGGTGATCCAGCAGCGCGGG
  ATAAAATTTGATGCTGGGACTCTCCTTCTTAGTACACACCGACTGATTTGGAGAGATCAGAAAAATCAT
  GAGTGTTGCATGGCCATTCTCCTTTCCCAAATTGTGTTCATTGAAGAACAGGCGGCTGGAATTGGGAAG
  AGTGCCAAAATAGTGGTTCATCTTCACCCAGCTCCTCCTAACAAAGAACCTGGCCCATTCCAGAGTAGT
  AAGAACTCCTACATCAAACTCTCCTTCAAAGAACATGGCCAGATTGAGTTTTACAGGCGTTTATCAGAG
  GAAATGACACAAAGAAGATGGGAGAATATGCCAGTTTCCCAGTCATTACAAACAAATAGAGGACCCCAG
  CCAGGAAGAATAAGGGCTGTAGGAATTGTAGGTATTGAAAGGAAACTGGAAGAAAAAAGAAAAGAAACT
  GACAAAAACATTTCTGAGGCCTTTGAAGACCTCAGCAAACTAATGATCAAGGCTAAGGAAATGGTGGAA
  TTATCAAAATCAATTGCTAATAAAATTAAAGACAAACAAGGTGACATCACAGAAGATGAGACCATCAGG
  TTTAAATCCTACTTGCTGAGCATGGGAATAGCTAACCCAGTTACCAGAGAAACCTACGGCTCAGGCACA
  CAGTACCACATGCAGCTGGCCAAACAACTGGCTGGAATATTGCAGGTGCCTTTAGAGGAACGAGGGGGA
  ATAATGTCACTCACGGAGGTGTACTGCTTAGTAAACCGAGCTCGAGGAATGGAATTGCTCTCACCAGAA
  GATTTAGTGAATGCGTGCAAGATGCTGGAAGCACTGAAATTACCTCTCAGGCTCCGTGTGTTTGACAGT
  GGCGTCATGGTAATTGAGCTTCAGTCTCACAAGGAAGAGGAAATGGTGGCCTCGGCCCTGGAGACAGTT
  TCAGAAAAGGGATCCCTAACATCAGAAGAGTTTGCTAAGCTTGTGGGAATGTCTGTCCTCCTAGCCAAA
  GAAAGGTTGCTGCTTGCAGAGAAGATGGGCCATCTTTGCCGTGATGACTCAGTGGAAGGCCTGCGTTTT
  TACCCAAATTTATTTATGACACAGAGCTAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001282168
• Insert Size: 1134 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001282168.1
• RefSeq Size: 4446 bp
• RefSeq ORF: 1134 bp
• Locus ID: 51028
• Cytogenetics: 13q14.3
• Protein Families: Transcription Factors
• Protein Pathways: Endocytosis
• MW: 42.7 kDa
• Gene Summary: This gene encodes a protein that is a subunit of the endosomal sorting complex required for transport II (ESCRT-II). This protein complex functions in sorting of ubiquitinated membrane proteins during endocytosis. A similar protein complex in rat is associated with RNA polymerase elongation factor II. [provided by RefSeq, Aug 2013]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' terminal exon compared to variant 1. This results in a shorter protein (isoform 2) compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.