MSL3L1 (MSL3) (NM_001282174) Human Untagged Clone

CAT#: SC335702

MSL3 (untagged) - Human male-specific lethal 3 homolog (Drosophila) (MSL3), transcript variant 6

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MSL3
• Synonyms: MRSXBA; MRXS36; MRXSBA; MSL3L1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC335702 representing NM_001282174.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAAAATGATGAAAACTAAAGAAGAACCAGAGCTTCAAACAAGAAGGGAAATGGAAGAAAGAACAATA
  ACTATAGAAATCCCTGAAGTTCTGAAGAAGCAGCTGGAGGATGATTGTTACTACATTAACAGGAGGAAA
  CGGTTAGTGAAACTTCCATGCCAGACCAACATCATAACGATTTTGGAATCCTATGTGAAGCATTTTGCT
  ATCAATGCAGCCTTTTCAGCCAATGAGAGGCCTCGTCACCATCACGTTATGCCACATGCCAACATGAAC
  GTGCATTATATCCCAGCAGAAAAGAATGTTGACCTTTGTAAGGAGATGGTGGATGGATTAAGAATAACC
  TTTGATTACACTCTCCCGTTGGTTTTACTCTATCCATATGAACAAGCTCAGTATAAAAAGGTGACTTCG
  TCTAAATTTTTTCTTCCAATTAAGGAAAGTGCCACAAGCACTAACAGGAGCCAGGAGGAACTCTCTCCC
  AGTCCGCCTTTGTTGAATCCATCCACGCCACAGTCCACAGAGAGTCAGCCGACCACCGGTGAACCAGCC
  ACCCCCAAAAGGCGCAAAGCTGAGCCAGAAGCATTGCAGTCTCTGAGGCGGTCCACGCGCCACAGTGCC
  AACTGTGACAGGCTTTCTGAGAGCAGCGCTTCACCTCAGCCCAAGCGCCGGCAGCAGGACACATCCGCC
  AGCATGCCCAAGCTCTTCCTGCACCTGGAAAAGAAGACACCTGTGCATAGCAGATCATCTTCACCTATT
  CCTCTGACTCCTAGCAAGGAAGGGAGTGCTGTGTTTGCTGGCTTTGAAGGGAGAAGAACTAATGAAATA
  AACGAGGTCCTCTCCTGGAAGCTTGTGCCTGACAATTACCCCCCAGGTGACCAGCCGCCTCCACCCTCT
  TACATTTATGGGGCACAACATTTGCTGCGATTGTTTGTGAAACTTCCAGAAATCCTTGGAAAGATGTCC
  TTTTCTGAGAAGAATCTGAAGGCTTTATTGAAGCACTTTGATCTCTTTTTGAGGTTTTTAGCAGAATAC
  CACGATGACTTCTTCCCAGAGTCGGCTTATGTCGCTGCCTGTGAGGCACATTACAGCACCAAGAACCCC
  CGGGCAATTTATTAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001282174
• Insert Size: 1119 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001282174.1
• RefSeq Size: 2276 bp
• RefSeq ORF: 1119 bp
• Locus ID: 10943
• UniProt ID: Q8N5Y2
• Cytogenetics: Xp22.2
• Protein Families: Transcription Factors
• MW: 42.7 kDa
• Gene Summary: This gene encodes a nuclear protein that is similar to the product of the Drosophila male-specific lethal-3 gene. The Drosophila protein plays a critical role in a dosage-compensation pathway, which equalizes X-linked gene expression in males and females. Thus, the human protein is thought to play a similar function in chromatin remodeling and transcriptional regulation, and it has been found as part of a complex that is responsible for histone H4 lysine-16 acetylation. This gene can undergo X inactivation. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 2, 7 and 8. [provided by RefSeq, Jul 2010]
  Transcript Variant: This variant (6) differs in its 5' UTR and 5' coding region, and uses a distinct start codon, compared to variant 1. The resulting isoform (f) has a distinct N-terminus and is shorter than isoform a.