B4GALNT1 (NM_001276468) Human Untagged Clone

CAT#: SC333255

B4GALNT1 (untagged) - Homo sapiens beta-1,4-N-acetyl-galactosaminyl transferase 1 (B4GALNT1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: B4GALNT1
• Synonyms: GALGT; GalNAc-T; GALNACT; SPG26
• Vector: pCMV6-Entry
• Sequence Data:
  Fully Sequenced ORF

  >SC333255 representing NM_001276468.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  ATGTGGCTGGGCCGCCGGGCCCTGTGCGCTCTGGTCCTTCTGCTCGCCTGCGCCTCGCTGGGGCTCCTG
  TACGCGAGCACCCGGGACGCGCCCGGCCTCCGGCTACCTCTTGCGCCGTGGGCGCCCCCGCAAAGCCCC
  CGCAGGCCCGAGCTGCCAGATCTTGCTCCTGAGCCCCGCTACGCACACATCCCGGTCAGGATCAAGGAG
  CAAGTAGTGGGGAGCCAGTCCCCAGCTGACCAGCTGCTCATAGCCCCTGCCAACTCCCCGCTCCAGTAC
  CCCCTACAGGGTGTGGAAGTTCAGCCCCTCAGGAGCATCTTGGTGCCAGGGCTGAGCCTTCAGGCAGCT
  TCTGGTCAGGAGGTATACCAGGTGAACCTGACTGCCTCCCTAGGCACCTGGGACGTGGCAGGGGAAGTG
  ACTGGAGTTACTCTCACTGGAGAGGGTCAGGCAGATCTCACCCTTGTCAGCCCAGGGCTGGACCAACTC
  AACAGGCAACTACAACTGGTCACTTACAGCAGCCGAAGCTACCAGACCAACACAGCAGACACAGTCCGG
  TTCTCCACCGAGGGACATGAGGCTGCTTTCACTATCCGCATAAGACACCCGCCCAACCCTCGGCTGTAC
  CCACCTGGGTCTCTACCCCAGGGAGCCCAGTACAACATCAGCGCTCTAGTCACGATTGCCACCAAGACC
  TTCCTCCGTTATGATCGGCTACGGGCTCTCATCACCAGTATCCGCCGCTTCTACCCAACGGTTACCGTG
  GTCATCGCTGACGACAGCGACAAGCCAGAGCGCGTTAGTGGCCCCTACGTGGAACACTATCTCATGCCC
  TTCGGCAAGGGCTGGTTCGCAGGCCGGAACCTGGCCGTGTCTCAAGTAACCACCAAGTACGTGCTGTGG
  GTGGACGACGACTTCGTCTTCACGGCGCGGACGCGGCTGGAGAGGCTTGTGGACGTGCTGGAGCGGACG
  CCGCTGGACCTGGTGGGGGGCGCGGTGCGCGAGATCTCCGGCTTTGCCACCACTTATCGGCAGCTGCTG
  AGCGTGGAGCCCGGCGCCCCAGGCCTCGGGAACTGCCTCCGGCAAAGGCGCGGCTTCCACCACGAGCTC
  GTCGGCTTCCCAGGCTGCGTGGTCACCGACGGCGTGGTTAACTTCTTCCTGGCGCGGACTGACAAGGTG
  CGCGAGGTCGGTTTCGACCCCCGCCTCAGCCGCGTGGCTCATCTGGAATTCTTCTTGGATGGGCTTGGT
  TCCCTTCGGGTTGGCTCCTGCTCCGACGTCGTGGTGGATCATGCATCCAAACTGAAGCTGCCTTGGACA
  TCAAGGGATGCCGGAGCAGAGACTTACGCCCGGTACCGTTACCCAGGATCACTGGACGAGAGCCAGATG
  GCCAAACACCGGCTGCTCTTCTTCAAACACCGGCTGCAGTGCATGACCTCCCAGTGA
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001276468
• Insert Size: 1437 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001276468.1
• RefSeq Size: 2428 bp
• RefSeq ORF: 1437 bp
• Locus ID: 2583
• UniProt ID: Q00973
• Cytogenetics: 12q13.3
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Glycosphingolipid biosynthesis - ganglio series, Metabolic pathways
• MW: 52.9 kDa
• Gene Summary: GM2 and GD2 gangliosides are sialic acid-containing glycosphingolipids. GalNAc-T is the enzyme involved in the biosynthesis of G(M2) and G(D2) glycosphingolipids. GalNAc-T catalyzes the transfer of GalNAc into G(M3) and G(D3) by a beta-1,4 linkage, resulting in the synthesis of G(M2) and G(D2), respectively. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2013]
  Transcript Variant: This variant (2) has an alternate exon in place of the first exon and lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.