SMARCAD1 (NM_001254949) Human Untagged Clone

CAT#: SC332272

SMARCAD1 (untagged) - Homo sapiens SWI/SNF-related, matrix-associated actin-dependent regulator of chromatin, subfamily a, containing DEAD/H box 1 (SMARCAD1), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SMARCAD1
• Synonyms: ADERM; BASNS; ETL1; HEL1; HRZ
• Vector: pCMV6-Entry
• Sequence Data:
  Fully Sequenced ORF

  >SC332272 representing NM_001254949.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  ATGTCCAAAACTAATGGCTTATCAGAAGATTTGATATGGCACTGTAAAACACTGATCCAAGAAAGAGAT
  GTAGTTATAAGGCTTATGAACAAATGTGAAGACATTTCAAATAAATTGACCAAACAAGTTACCATGCTT
  ACTGGAAATGGAGGTGGATGGAACATAGAACAACCTTCCATTCTAAACCAAAGTTTGTCACTCAAGCCC
  TATCAGAAGGTTGGTTTGAATTGGCTGGCATTGGTACATAAACATGGACTTAATGGCATTTTGGCAGAT
  GAAATGGGCCTAGGAAAAACTATTCAAGCCATTGCATTTCTGGCATACCTCTATCAGGAGGGTAATAAT
  GGTCCTCATTTGATCGTTGTTCCAGCTTCAACTATAGATAACTGGTTAAGGGAAGTTAATTTATGGTGC
  CCTACTTTGAAGGTCCTCTGTTACTATGGTTCTCAAGAAGAACGTAAACAAATTAGATTTAACATTCAT
  AGTAGATATGAAGATTACAATGTAATTGTGACCACATATAACTGTGCGATCAGCAGTTCTGATGACCGT
  AGTCTGTTTCGACGGCTGAAACTTAATTACGCAATTTTTGATGAGGGCCATATGCTGAAGAATATGGGC
  TCCATTCGCTACCAGCACCTTATGACAATTAATGCAAATAACCGTTTGCTGCTCACAGGCACACCTGTA
  CAGAACAATCTGTTAGAACTCATGTCGCTGTTGAATTTTGTTATGCCACACATGTTTAGTAGTAGCACC
  AGTGAAATACGAAGAATGTTTTCCTCTAAGACAAAATCAGCAGATGAGCAAAGCATATATGAAAAGGAG
  AGAATAGCACATGCAAAACAAATTATAAAGCCATTTATTCTCAGAAGAGTAAAAGAAGAGGTTCTCAAG
  CAGTTACCCCCCAAGAAAGATCGAATTGAGTTGTGTGCAATGTCGGAGAAGCAGGAGCAACTCTATTTG
  GGTCTTTTCAACAGATTGAAAAAATCTATCAATAACTTGGAAAAAAACACAGAAATGTGCAATGTCATG
  ATGCAGTTGAGGAAAATGGCCAATCATCCTTTATTACATCGCCAATATTACACAGCTGAAAAACTCAAG
  GAAATGTCTCAGCTTATGCTAAAGGAACCTACACATTGTGAGGCTAACCCTGACCTGATCTTTGAAGAT
  ATGGAAGTTATGACAGACTTCGAACTACATGTACTTTGTAAACAGTACCGACACATTAATAACTTTCAG
  TTAGACATGGACTTGATTTTAGATTCTGGAAAATTTCGAGTTTTAGGATGCATCTTGTCTGAATTGAAA
  CAGAAGGGTGATAGAGTTGTGTTATTTAGCCAATTTACCATGATGCTGGATATCTTAGAGGTTCTATTA
  AAACATCATCAGCATAGGTACCTCAGATTAGATGGAAAGACTCAGATTTCTGAAAGGATTCATCTAATT
  GATGAGTTTAATACCGATATGGATATCTTTGTGTTTCTGCTATCAACAAAAGCTGGTGGATTAGGAATA
  AATCTGACTTCAGCAAATGTTGTTATACTTCACGATATTGACTGTAATCCTTATAATGACAAACAAGCA
  GAAGATAGATGCCATAGAGTAGGCCAGACTAAAGAAGTACTAGTTATAAAACTAATAAGCCAAGGGACG
  ATTGAAGAATCCATGCTAAAAATTAACCAACAGAAATTGAAACTAGAACAGGATATGACTACAGTAGAT
  GAAGGTGATGAAGGGAGTATGCCAGCAGATATAGCCACATTACTAAAAACATCAATGGGCCTGTGA
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001254949
• Insert Size: 1791 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001254949.1
• RefSeq Size: 3950 bp
• RefSeq ORF: 1791 bp
• Locus ID: 56916
• UniProt ID: Q9H4L7
• Cytogenetics: 4q22.3
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• MW: 68.8 kDa
• Gene Summary: This gene encodes a member of the SNF subfamily of helicase proteins. The encoded protein plays a critical role in the restoration of heterochromatin organization and propagation of epigenetic patterns following DNA replication by mediating histone H3/H4 deacetylation. Mutations in this gene are associated with adermatoglyphia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]
  Transcript Variant: This variant (4) differs in the 5' UTR, uses an alternate splice site in the coding region and initiates translation at a downstream, in-frame start codon, compared to variant 1. The encoded isoform (c) is shorter than isoform a.