NFIC (NM_001245002) Human Untagged Clone

CAT#: SC332114

NFIC (untagged) - Homo sapiens nuclear factor I/C (CCAAT-binding transcription factor) (NFIC), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NFIC
• Synonyms: CTF; CTF5; NF-I; NFI
• Vector: pCMV6-Entry
• Sequence Data:
  Fully Sequenced ORF

  >SC332114 representing NM_001245002.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  ATGTATTCGTCCCCGCTCTGCCTCACCCAGGATGAGTTCCACCCGTTCATCGAGGCCCTGCTGCCTCAC
  GTCCGCGCCTTCGCCTACACCTGGTTCAACCTGCAGGCGCGGAAGCGCAAGTACTTCAAGAAGCACGAG
  AAGCGGATGTCGAAGGACGAGGAGCGTGCGGTCAAGGACGAGCTGCTGGGCGAGAAGCCCGAGGTCAAG
  CAGAAGTGGGCGTCGCGGCTGCTGGCCAAGCTGCGCAAGGACATCCGGCCCGAGTGCCGCGAGGACTTC
  GTGCTGAGCATCACCGGCAAGAAGGCGCCGGGCTGCGTGCTCTCCAACCCCGACCAGAAGGGCAAGATG
  CGGCGCATCGACTGTCTCCGGCAGGCGGACAAGGTGTGGCGGCTGGACCTGGTCATGGTCATCCTGTTC
  AAGGGCATCCCGCTGGAGAGCACCGACGGCGAGCGCCTGGTCAAGGCTGCGCAGTGCGGTCACCCGGTC
  CTGTGCGTGCAGCCGCACCACATTGGCGTGGCCGTCAAGGAGCTGGACCTCTACCTGGCCTACTTCGTG
  CGTGAGCGAGATGCAGAGCAAAGCGGCAGTCCCCGGACAGGGATGGGCTCTGACCAGGAGGACAGCAAG
  CCCATCACGCTGGACACGACCGACTTCCAGGAGAGCTTTGTCACCTCCGGCGTGTTCAGCGTCACTGAG
  CTCATCCAAGTGTCCCGGACACCCGTGGTGACTGGAACAGGACCCAACTTCTCCCTGGGGGAGCTGCAG
  GGGCACCTGGCATACGACCTGAACCCAGCCAGCACTGGCCTCAGAAGAACGCTGCCCAGCACCTCCTCC
  AGTGGGAGCAAGCGGCACAAATCGGGCTCGATGGAGGAAGACGTGGACACGAGCCCTGGCGGCGATTAC
  TACACTTCGCCCAGCTCGCCCACGAGTAGCAGCCGCAACTGGACGGAGGACATGGAAGGAGGCATCTCG
  TCCCCGGTGAAGAAGACAGAGATGGACAAGTCACCATTCAACAGCCCGTCCCCCCAGGACTCTCCCCGC
  CTCTCCAGCTTCACCCAGCACCACCGGCCCGTCATCGCCGTGCACAGCGGGATCGCCCGGAGCCCACAC
  CCGTCCTCCGCTCTGCATTTCCCTACGACGTCCATCCTACCCCAGACGGCCTCCACCTACTTCCCCCAC
  ACGGCCATCCGCTACCCACCTCATCTCAACCCCCAGGACCCGCTCAAAGATCTTGTCTCGCTGGCCTGC
  GACCCAGCCAGCCAGCAACCTGGACCGTTAAATGGAAGTGGTCAGCTCAAAATGCCCAGCCACTGCCTT
  TCTGCTCAGATGCTGGCACCTCCGCCCCCGGGGCTGCCACGGCTGGCGCTCCCCCCTGCCACCAAACCC
  GCCACCACCTCCGAGGGAGGAGCCACGTCGCCGACCTCGCCTTCCTACTCTCCGCCCGACACGTCCCCT
  GCAAACCGTTCCTTTGTGGGATTAGGACCAAGGGATCCTGCGGGCATTTATCAGGCACAGTCCTGGTAT
  CTGGGATAG
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001245002
• Insert Size: 1527 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_001245002.1
• RefSeq Size: 8063 bp
• RefSeq ORF: 1527 bp
• Locus ID: 4782
• UniProt ID: P08651
• Cytogenetics: 19p13.3
• Protein Families: Transcription Factors
• MW: 55.7 kDa
• Gene Summary: The protein encoded by this gene belongs to the CTF/NF-I family. These are dimeric DNA-binding proteins, and function as cellular transcription factors and as replication factors for adenovirus DNA replication. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011]
  Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.