PDE7A (NM_001242318) Human Untagged Clone

CAT#: SC331787

PDE7A (untagged) - Homo sapiens phosphodiesterase 7A (PDE7A), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PDE7A
• Synonyms: HCP1; PDE7
• Vector: pCMV6-Entry
• Sequence Data:
  Fully Sequenced ORF

  >SC331787 representing NM_001242318.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  ATGGAAGTGTGTTACCAGCTGCCGGTACTGCCCCTGGACAGGCCGGTCCCCCAGCACGTCCTCAGCCGC
  CGAGGAGCCATCAGCTTCAGCTCCAGCTCCGCTCTCTTCGGCTGCCCCAATCCCCGGCAGCTCTCTCAG
  AGGCGTGGAGCTATTTCCTATGACAGTTCTGATCAGACTGCATTATACATTCGTATGCTAGGAGATGTA
  CGTGTAAGGAGCCGAGCAGGATTTGAATCAGAAAGAAGAGGTTCTCACCCATATATTGATTTTCGTATT
  TTCCACTCTCAATCTGAAATTGAAGTGTCTGTCTCTGCAAGGAATATCAGAAGGCTACTAAGTTTCCAG
  CGATATCTTAGATCTTCACGCTTTTTTCGTGGTACTGCGGTTTCAAATTCCCTAAACATTTTAGATGAT
  GATTATAATGGACAAGCCAAGTGTATGCTGGAAAAAGTTGGAAATTGGAATTTTGATATCTTTCTATTT
  GATAGACTAACAAATGGAAATAGTCTAGTAAGCTTAACCTTTCATTTATTTAGTCTTCATGGATTAATT
  GAGTACTTCCATTTAGATATGATGAAACTTCGTAGATTTTTAGTTATGATTCAAGAAGATTACCACAGT
  CAAAATCCTTACCATAACGCAGTCCACGCTGCGGATGTTACTCAGGCCATGCACTGTTACTTAAAGGAA
  CCTAAGCTTGCCAATTCTGTAACTCCTTGGGATATCTTGCTGAGCTTAATTGCAGCTGCCACTCATGAT
  CTGGATCATCCAGGTGTTAATCAACCTTTCCTTATTAAAACTAACCATTACTTGGCAACTTTATACAAG
  AATACCTCAGTACTGGAAAATCACCACTGGAGATCTGCAGTGGGCTTATTGAGAGAATCAGGCTTATTC
  TCACATCTGCCATTAGAAAGCAGGCAACAAATGGAGACACAGATAGGTGCTCTGATACTAGCCACAGAC
  ATCAGTCGCCAGAATGAGTATCTGTCTTTGTTTAGGTCCCATTTGGATAGAGGTGATTTATGCCTAGAA
  GACACCAGACACAGACATTTGGTTTTACAGATGGCTTTGAAATGTGCTGATATTTGTAACCCATGTCGG
  ACGTGGGAATTAAGCAAGCAGTGGAGTGAAAAAGTAACGGAGGAATTCTTCCATCAAGGAGATATAGAA
  AAAAAATATCATTTGGGTGTGAGTCCACTTTGCGATCGTCACACTGAATCTATTGCCAACATCCAGATT
  GGTTTTATGACTTACCTAGTGGAGCCTTTATTTACAGAATGGGCCAGGTTTTCCAATACAAGGCTATCC
  CAGACAATGCTTGGACACGTGGGGCTGAATAAAGCCAGCTGGAAGGGACTGCAGAGAGAACAGTCGAGC
  AGTGAGGACACTGATGCTGCATTTGAGTTGAACTCACAGTTATTACCTCAGGAAAATCGGTTATCATAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001242318
• Insert Size: 1449 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001242318.2
• RefSeq Size: 6641 bp
• RefSeq ORF: 1449 bp
• Locus ID: 5150
• UniProt ID: Q13946
• Cytogenetics: 8q13.1
• Protein Families: Druggable Genome
• Protein Pathways: Progesterone-mediated oocyte maturation, Purine metabolism
• MW: 55.5 kDa
• Gene Summary: The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE7 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2011]
  Transcript Variant: This variant (3, also called PDE7A1) represents the longer transcript, and encodes the longer isoform (c). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.