Calpain 1 (CAPN1) (NM_001198868) Human Untagged Clone

CAT#: SC331231

CAPN1 (untagged) - Homo sapiens calpain 1, (mu/I) large subunit (CAPN1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Calpain 1
• Synonyms: CANP; CANP1; CANPL1; muCANP; muCL; SPG76
• Vector: pCMV6-Entry
• Sequence Data:
  Fully Sequenced ORF

  >SC331231 representing NM_001198868.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  ATGTCGGAGGAGATCATCACGCCGGTGTACTGCACTGGGGTGTCAGCCCAAGTGCAGAAGCAGCGGGCC
  AGGGAGCTGGGCCTGGGCCGCCATGAGAATGCCATCAAGTACCTGGGCCAGGATTATGAGCAGCTGCGG
  GTGCGATGCCTGCAGAGTGGGACCCTCTTCCGTGATGAGGCCTTCCCCCCGGTACCCCAGAGCCTGGGT
  TACAAGGACCTGGGTCCCAATTCCTCCAAGACCTATGGCATCAAGTGGAAGCGTCCCACGGAACTGCTG
  TCAAACCCCCAGTTCATTGTGGATGGAGCTACCCGCACAGACATCTGCCAGGGAGCACTGGGGGACTGC
  TGGCTCTTGGCGGCCATCGCCTCCCTCACTCTCAACGACACCCTCCTGCACCGAGTGGTTCCGCACGGC
  CAGAGCTTCCAGAATGGCTATGCCGGCATCTTCCATTTCCAGCTGTGGCAATTTGGGGAGTGGGTGGAC
  GTGGTCGTGGATGACCTGCTGCCCATCAAGGACGGGAAGCTAGTGTTCGTGCACTCTGCCGAAGGCAAC
  GAGTTCTGGAGCGCCCTGCTTGAGAAGGCCTATGCCAAGGTAAATGGCAGCTACGAGGCCCTGTCAGGG
  GGCAGCACCTCAGAGGGCTTTGAGGACTTCACAGGCGGGGTTACCGAGTGGTACGAGTTGCGCAAGGCT
  CCCAGTGACCTCTACCAGATCATCCTCAAGGCGCTGGAGCGGGGCTCCCTGCTGGGCTGCTCCATAGAC
  ATCTCCAGCGTTCTAGACATGGAGGCCATCACTTTCAAGAAGTTGGTGAAGGGCCATGCCTACTCTGTG
  ACCGGGGCCAAGCAGGTGAACTACCGAGGCCAGGTGGTGAGCCTGATCCGGATGCGGAACCCCTGGGGC
  GAGGTGGAGTGGACGGGAGCCTGGAGCGACAGCTCCTCAGAGTGGAACAACGTGGACCCATATGAACGG
  GACCAGCTCCGGGTCAAGATGGAGGACGGGGAGTTCTGGATGTCATTCCGAGACTTCATGCGGGAGTTC
  ACCCGCCTGGAGATCTGCAACCTCACACCCGACGCCCTCAAGAGCCGGACCATCCGCAAATGGAACACC
  ACACTCTACGAAGGCACCTGGCGGCGGGGGAGCACCGCGGGGGGCTGCCGAAACTACCCAGCCACCTTC
  TGGGTGAACCCTCAGTTCAAGATCCGGCTGGATGAGACGGATGACCCGGACGACTACGGGGACCGCGAG
  TCAGGCTGCAGCTTCGTGCTCGCCCTTATGCAGAAGCACCGTCGCCGCGAGCGCCGCTTCGGCCGCGAC
  ATGGAGACTATTGGCTTCGCGGTCTACGAGGTCCCTCCGGAGCTGGTGGGCCAGCCGGCCGTACACTTG
  AAGCGTGACTTCTTCCTGGCCAATGCGTCTCGGGCGCGCTCAGAGCAGTTCATCAACCTGCGAGAGGTC
  AGCACCCGCTTCCGCCTGCCACCCGGGGAGTATGTGGTGGTGCCCTCCACCTTCGAGCCCAACAAGGAG
  GGCGACTTCGTGCTGCGCTTCTTCTCAGAGAAGAGTGCTGGGACTGTGGAGCTGGATGACCAGATCCAG
  GCCAATCTCCCCGATGAGCAAGTGCTCTCAGAAGAGGAGATTGACGAGAACTTCAAGGCCCTCTTCAGG
  CAGCTGGCAGGGGAGGACATGGAGATCAGCGTGAAGGAGTTGCGGACAATCCTCAATAGGATCATCAGC
  AAACACAAAGACCTGCGGACCAAGGGCTTCAGCCTAGAGTCGTGCCGCAGCATGGTGAACCTCATGGAT
  CGTGATGGCAATGGGAAGCTGGGCCTGGTGGAGTTCAACATCCTGTGGAACCGCATCCGGAATTACCTG
  TCCATCTTCCGGAAGTTTGACCTGGACAAGTCGGGCAGCATGAGTGCCTACGAGATGCGGATGGCCATT
  GAGTCGGCAGGCTTCAAGCTCAACAAGAAGCTGTACGAGCTCATCATCACCCGCTACTCGGAGCCCGAC
  CTGGCGGTCGACTTTGACAATTTCGTTTGCTGCCTGGTGCGGCTAGAGACCATGTTCCGATTTTTCAAA
  ACTCTGGACACAGATCTGGATGGAGTTGTGACCTTTGACTTGTTTAAGTGGTTGCAGCTGACCATGTTT
  GCATGA
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001198868
• Insert Size: 2145 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001198868.1
• RefSeq Size: 3125 bp
• RefSeq ORF: 2145 bp
• Locus ID: 823
• UniProt ID: P07384
• Cytogenetics: 11q13.1
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Alzheimer's disease, Apoptosis
• MW: 81.9 kDa
• Gene Summary: The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Variants 1, 2, and 3 all encode the same protein.