ORC1 (NM_001190818) Human Untagged Clone

CAT#: SC331138

ORC1 (untagged) - Homo sapiens origin recognition complex, subunit 1 (ORC1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ORC1
• Synonyms: HSORC1; ORC1L; PARC1
• Vector: pCMV6-Entry
• Sequence Data:
  Fully Sequenced ORF

  >SC331138 representing NM_001190818.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  ATGGCACACTACCCCACAAGGCTGAAGACCAGAAAAACTTATTCATGGGTTGGCAGGCCCTTGTTGGAT
  CGAAAACTGCACTACCAAACCTATAGAGAAATGTGTGTGAAAACAGAAGGTTGTTCCACCGAGATTCAC
  ATCCAGATTGGACAGTTTGTGTTGATTGAAGGGGATGATGATGAAAACCCGTATGTTGCTAAATTGCTT
  GAGTTGTTCGAAGATGACTCTGATCCTCCTCCTAAGAAACGTGCTCGAGTACAGTGGTTTGTCCGATTC
  TGTGAAGTCCCTGCCTGTAAACGGCATTTGTTGGGCCGGAAGCCTGGTGCACAGGAAATATTCTGGTAT
  GATTACCCGGCCTGTGACAGCAACATTAATGCGGAGACCATCATTGGCCTTGTTCGGGTGATACCTTTA
  GCCCCAAAGGATGTGGTACCGACGAATCTGAAAAATGAGAAGACACTCTTTGTGAAACTATCCTGGAAT
  GAGAAGAAATTCAGGCCACTTTCCTCAGAACTATTTGCGGAGTTGAATAAACCACAAGAGAGTGCAGCC
  AAGTGCCAGAAACCCGTGAGAGCCAAGAGTAAGAGTGCAGAGAGCCCTTCTTGGACCCCAGCAGAACAT
  GTGGCCAAAAGGATTGAATCAAGGCACTCCGCCTCCAAATCTCGCCAAACTCCTACCCATCCTCTTACC
  CCAAGAGCCAGAAAGAGGCTGGAGCTTGGCAACTTAGGTAACCCTCAGATGTCCCAGCAGACTTCATGT
  GCCTCCTTGGATTCTCCAGGAAGAATAAAACGGAAAGTGGCCTTCTCGGAGATCACCTCACCTTCTAAG
  AGATCTCAGCCTGATAAACTTCAAACCTTGTCTCCAGCTCTGAAAGCCCCAGAGAAAACCAGAGAGACT
  GGACTCTCTTATACTGAGGATGACAAGAAGGCTTCACCTGAACATCGCATAATCCTGAGAACCCGAATT
  GCAGCTTCGAAAACCATAGACATTAGAGAGGAGAGAACACTTACCCCTATCAGTGGGGGACAGAGATCT
  TCAGTGGTGCCATCCGTGATTCTGAAACCAGAAAACATCAAAAAGAGGGATGCAAAAGAAGCAAAAGCC
  CAGAATGAAGCGACCTCTACTCCCCATCGTATCCGCAGAAAGAGTTCTGTCTTGACTATGAATCGGATT
  AGGCAGCAGCTTCGGTTTCTAGGTAATAGTAAAAGTGACCAAGAAGAGAAAGAGATTCTGCCAGCAGCA
  GAGATTTCAGACTCTAGCAGTGACGAAGAAGAGGCTTCCACACCGCCCCTTCCAAGGAGAGCACCCAGA
  ACTGTGTCCAGGAACCTGCGATCTTCCTTGAAGTCATCCTTACATACCCTCACGAAGGTGCCAAAGAAG
  AGTCTCAAGCCTAGAACGCCACGTTGTGCCGCTCCTCAGATCCGTAGTCGAAGCCTGGCTGCCCAGGAG
  CCAGCCAGTGTGCTGGAGGAAGCCCGACTGAGGCTGCATGTTTCTGCTGTACCTGAGTCTCTTCCCTGT
  CGGGAACAGGAATTCCAAGACATCTACAATTTTGTGGAAAGCAAACTCCTTGACCATACCGGAGGGTGC
  ATGTACATCTCCGGTGTCCCTGGGACAGGGAAGACTGCCACTGTTCATGAAGTGATACGCTGCCTGCAG
  CAGGCAGCCCAAGCCAATGATGTTCCTCCCTTTCAATACATTGAGGTCAATGGCATGAAGCTGACGGAG
  CCCCACCAAGTCTATGTGCAAATCTTGCAGAAGCTAACAGGCCAAAAAGCAACAGCCAACCATGCGGCA
  GAACTGCTGGCAAAGCAATTCTGCACCCGAGGGTCACCTCAGGAAACCACCGTCCTGCTTGTGGATGAG
  CTCGACCTTCTGTGGACTCACAAACAAGACATAATGTACAATCTCTTTGACTGGCCCACTCATAAGGAG
  GCCCGGCTTGTGGTCCTGGCAATTGCCAACACAATGGACCTGCCAGAGCGAATCATGATGAACCGGGTG
  TCCAGCCGACTGGGTCTTACCAGGATGTGCTTCCAGCCCTATACATATAGCCAGCTGCAGCAGATCCTA
  AGGTCCCGGCTCAAGCATCTAAAGGCCTTTGAAGATGATGCCATCCAGCTGGTAGCCAGGAAGGTAGCA
  GCACTGTCTGGAGATGCACGACGGTGCCTGGACATCTGCAGGCGTGCCACAGAGATCTGTGAGTTCTCC
  CAGCAGAAGCCTGACTCCCCTGGCCTGGTCACCATAGCCCACTCAATGGAAGCTGTGGATGAGATGTTT
  TCATCATCATACATCACGGCCATCAAAAATTCCTCTGTTCTGGAACAGAGCTTCCTGAGAGCCATCCTC
  GCAGAGTTCCGTCGATCAGGACTGGAGGAAGCCACGTTTCAACAGATATATAGTCAACATGTGGCACTG
  TGCAGAATGGAGGGACTGCCGTACCCCACCATGTCAGAGACCATGGCCGTGTGTTCTCACCTGGGCTCC
  TGTCGCCTCCTGCTTGTGGAGCCCAGCAGGAACGATCTGCTCCTTCGGGTGCGGCTCAACGTCAGCCAG
  GATGATGTGCTGTATGCGCTGAAAGACGAGTAA
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001190818
• Insert Size: 2586 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001190818.1
• RefSeq Size: 3189 bp
• RefSeq ORF: 2586 bp
• Locus ID: 4998
• UniProt ID: Q13415
• Cytogenetics: 1p32.3
• Protein Families: Stem cell - Pluripotency
• Protein Pathways: Cell cycle
• MW: 97.3 kDa
• Gene Summary: The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is the largest subunit of the ORC complex. While other ORC subunits are stable throughout the cell cycle, the levels of this protein vary during the cell cycle, which has been shown to be controlled by ubiquitin-mediated proteolysis after initiation of DNA replication. This protein is found to be selectively phosphorylated during mitosis. It is also reported to interact with MYST histone acetyltransferase 2 (MyST2/HBO1), a protein involved in control of transcription silencing. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]
  Transcript Variant: This variant (2) has an alternate splice site in the 5' UTR, resulting in 3 nt fewer than variant 1. Variants 1 and 2 encode the same isoform 1.