BCS1L (NM_001257343) Human Untagged Clone

CAT#: SC330605

BCS1L (untagged) - Homo sapiens BC1 (ubiquinol-cytochrome c reductase) synthesis-like (BCS1L), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BCS1L
• Synonyms: BCS; BCS1; BJS; FLNMS; GRACILE; h-BCS; h-BCS1; Hs.6719; MC3DN1; PTD
• Vector: pCMV6-Entry
• Sequence Data:
  Fully Sequenced ORF

  >SC330605 representing NM_001257343.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  ATGCCACTTTCAGACTTTATTCTGGCTCTGAAGGACAATCCCTACTTTGGGGCTGGATTTGGGCTGGTG
  GGTGTGGGCACAGCCCTGGCCCTGGCCCGGAAGGGTGTCCAACTGGGCCTGGTGGCATTCCGGCGCCAT
  TACATGATCACACTGGAAGTCCCTGCTCGAGACAGGAGCTATGCCTGGTTGCTTAGCTGGCTCACCCGC
  CACAGTACCCGTACTCAGCACCTCAGTGTCGAGACTTCGTACCTTCAGCATGAGAGTGGCCGCATTTCC
  ACTAAGTTTGAATTTGTCCCCAGCCCTGGAAACCATTTTATCTGGTATCGGGGGAAATGGATTCGGGTA
  GAACGAAGTCGAGAGATGCAGATGATAGACTTGCAGACGGGGACTCCTTGGGAATCTGTCACCTTCACG
  GCCCTGGGCACTGACCGAAAGGTTTTCTTCAACATCCTGGAGGAAGCTCGAGAGCTAGCCTTGCAGCAG
  GAGGAAGGGAAGACCGTGATGTACACAGCTGTGGGCTCTGAATGGCGTCCCTTTGGCTATCCACGCCGC
  CGGCGACCACTGAATTCTGTGGTTCTACAACAGGGTCTGGCTGACCGAATTGTCAGAGACGTCCAGGAA
  TTCATCGATAACCCCAAGTGGTACACTGACAGAGGCATTCCTTACAGACGTGGCTACCTGCTTTATGGG
  CCCCCTGGTTGCGGAAAGAGCAGTTTTATCACAGCCCTGGCTGGGGAACTGGAGCACAGCATCTGCCTG
  CTGAGCCTCACGGACTCCAGCCTCTCTGATGACCGACTCAACCACCTGCTGAGCGTGGCCCCGCAGCAG
  AGCCTGGTACTCCTGGAGGATGTGGATGCTGCTTTTCTCAGTCGAGACTTGGCTGTGGAGAACCCAGTA
  AAGTACCAAGGCCTAGGTCGCCTCACCTTCAGTGGACTGCTCAATGCCTTGGATGGTGTGGCTTCCACC
  GAGGCCCGCATCGTGTTCATGACCACCAACCACGTTGACAGGCTGGACCCTGCCCTGATACGCCCGGGG
  CGAGTGGACCTGAAGGAGTACGTGGGCTACTGCTCACACTGGCAGCTGACCCAGATGTTCCAGAGGTTC
  TATCCAGGGCAGGCACCTTCCTTAGCTGAGAACTTTGCAGAACATGTCCTTCGAGCTACAAACCAGATC
  AGTCCTGCCCAGGTGCAGGGCTACTTCATGCTGTATAAAAATGACCCTGTAGGGGCAATTCACAATGCT
  GAGTCTCTGAGGAGGTGA
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001257343
• Insert Size: 1260 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001257343.1
• RefSeq Size: 1636 bp
• RefSeq ORF: 1260 bp
• Locus ID: 617
• UniProt ID: Q9Y276
• Cytogenetics: 2q35
• Protein Families: Druggable Genome
• MW: 47.5 kDa
• Gene Summary: This gene encodes a homolog of the S. cerevisiae bcs1 protein which is involved in the assembly of complex III of the mitochondrial respiratory chain. The encoded protein does not contain a mitochondrial targeting sequence but experimental studies confirm that it is imported into mitochondria. Mutations in this gene are associated with mitochondrial complex III deficiency and the GRACILE syndrome. Several alternatively spliced transcripts encoding two different isoforms have been described. [provided by RefSeq, Jan 2016]
  Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1. Variants 1, 2, 3, 4, 5, and 7 all encode the same isoform (a).