DGKH (NM_001204506) Human Untagged Clone

CAT#: SC329760

DGKH (untagged) - Homo sapiens diacylglycerol kinase, eta (DGKH), transcript variant 5

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DGKH
• Synonyms: DGKeta
• Vector: pCMV6-Entry
• Sequence Data:
  Fully Sequenced ORF

  >SC329760 representing NM_001204506.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  ATGCTGTGTGCTGAGAACAGAAAGGAGATGGAGGATTGGATCAGCTCACTGAAGTCTGTACAGACCAGA
  GAACCCTACGAGGTGGCCCAGTTTAATGTGGAACATTTCTCAGGGATGCACAACTGGTACGCCTGCTCC
  CACGCCCGACCCACCTTCTGTAACGTGTGCAGAGAGAGTCTTTCTGGAGTCACCTCCCATGGCCTGTCC
  TGCGAAGTGTGTAAATTCAAGGCTCACAAAAGATGTGCAGTGAGAGCAACAAATAACTGTAAATGGACT
  ACCCTGGCCTCCATCGGGAAGGACATTATAGAAGATGAAGATGGCGTCGCGATGCCTCACCAGTGGCTT
  GAGGGCAACCTGCCTGTAAGTGCCAAGTGTGCTGTCTGCGACAAAACATGTGGCAGTGTTCTCCGTCTA
  CAGGATTGGAAATGCCTTTGGTGTAAGACAATGGTACACACTGCCTGCAAAGATTTATACCATCCAATA
  TGTCCACTTGGTCAATGTAAAGTATCTATCATACCTCCAATTGCACTAAACAGCACCGATTCCGATGGT
  TTCTGTAGAGCAACATTTTCGTTCTGTGTTAGTCCTCTATTGGTTTTTGTCAATTCTAAGAGTGGAGAT
  AATCAGGGAGTAAAGTTCCTCCGTCGCTTTAAACAGTTGCTAAATCCGGCTCAGGTGTTTGATTTAATG
  AATGGAGGTCCTCATTTAGGTTTAAGATTATTTCAGAAGTTTGACAATTTCCGGATTCTTGTTTGTGGA
  GGCGATGGAAGTGTAGGTTGGGTTTTGTCAGAAATCGATAAGCTCAACTTGAATAAACAGTGTCAGCTG
  GGAGTGTTGCCTTTGGGTACAGGAAATGACCTTGCCCGAGTTCTTGGCTGGGGAGGTTCATATGACGAT
  GACACCCAACTTCCTCAGATCCTAGAGAAACTGGAACGAGCCAGTACCAAAATGTTGGACAGGTGGAGT
  ATAATGACATATGAACTCAAATTGCCACCAAAAGCTTCCCTACTTCCAGGACCTCCAGAAGCATCTGAA
  GAATTTTATATGACGATTTATGAAGACTCAGTTGCAACGCATCTTACAAAAATCCTCAATTCTGATGAA
  CATGCAGTGGTCATATCTTCTGCCAAGACGCTATGTGAAACTGTAAAGGACTTCGTTGCCAAAGTAGAA
  AAGACGTATGACAAAACCTTGGAAAATGCCGTTGTAGCTGATGCCGTGGCCAGTAAATGTTCAGTCCTA
  AACGAGAAGCTCGAACAACTGCTGCAGGCTTTGCACACAGATTCCCAGGCTGCGCCTGTTCTCCCTGGC
  CTCAGCCCTCTCATTGTGGAAGAAGATGCTGTGGAATCGTCCAGTGAAGAGTCCCTGGGTGAAAGCAAA
  GAGCAGCTTGGGGATGACGTTACAAAACCTTCCTCCCAGAAAGCCGTCAAACCAAGGGAAATCATGTTG
  CGGGCAAATAGTTTAAAGAAAGCAGTGAGGCAAGTCATTGAGGAAGCCGGAAAAGTTATGGATGACCCG
  ACAGTTCACCCCTGTGAACCAGCTAATCAGTCCTCTGATTATGACAGCACAGAAACAGATGAATCTAAG
  GAGGAAGCTAAAGATGATGGTGCCAAAGAATCAATAACTGTTAAAACTGCACCTCGGTCTCCAGATGCC
  CGGGCAAGTTATGGCCATTCCCAAACTGATTCTGTCCCTGGTCCAGCTGTGGCAGCCAGCAAAGAAAAC
  CTCCCTGTGCTCAATACCAGAATAATCTGCCCAGGTTTAAGAGCAGGACTGGCTGCCTCAATTGCTGGG
  AGTTCGATTATCAACAAAATGTTACTGGCAAACATTGATCCTTTTGGTGCCACGCCGTTTATTGACCCG
  GATCTAGATTCCGTAGATGGATATTCAGAAAAATGTGTCATGAACAATTACTTTGGGATTGGATTAGAT
  GCAAAAATTTCATTAGAATTTAATAATAAAAGAGAGGAGCACCCTGAAAAATGCAGGAGCCGAACTAAA
  AACTTGATGTGGTATGGAGTCCTTGGAACCCGGGAGTTATTACAGAGATCGTACAAGAATTTAGAACAA
  AGGGTTCAACTTGAGTGTGATGGGCAGTATATTCCTCTTCCCAGCTTGCAAGGCATAGCCGTGTTGAAC
  ATTCCCAGCTATGCTGGAGGCACTAACTTTTGGGGTGGAACTAAAGAGGATGATATATTTGCTGCACCA
  TCCTTTGATGACAAGATCCTGGAAGTTGTAGCAATATTTGATAGCATGCAAATGGCAGTTTCAAGGGTC
  ATTAAACTGCAGCATCATCGAATAGCCCAGTGCCGTACAGTGAAAATCACTATATTTGGTGACGAAGGA
  GTCCCAGTGCAAGTGGATGGTGAAGCGTGGGTTCAGCCTCCAGGGATTATCAAAATTGTGCACAAAAAC
  AGAGCACAAATGCTAACAAGGGACAGAGCCTTTGAGAGCACTCTGAAATCTTGGGAAGATAAGCAGAAG
  TGTGATTCTGGTAAACCAGTTCTCCGAACCCATTTGTACATCCATCACGCCATTGACTTGGCAACAGAA
  GAGGTGTCGCAGATGCAGCTATGCTCCCAGGCTGCAGAGGAGCTCATTACTAGGATATGTGACGCAGCC
  ACAATTCACTGTCTTTTGGAGCAAGAACTGGCCCATGCTGTGAATGCCTGCTCCCATGCCCTGAATAAA
  GCCAACCCAAGGTGCCCGGAGAGTCTTACAAGAGACACTGCCACTGAAATAGCCATCAATGTGAAGGCG
  CTGTATAATGAAACAGAATCTTTGCTAGTTGGCAGGGTTCCTTTGCAGCTGGAATCGCCACATGAAGAG
  CGAGTATCCAATGCCTTACACTCTGTGGAGGTGGAATTACAGAAACTGACAGAGATTCCTTGGCTTTAT
  TATATCTTACACCCAAATGAGGATGAGGAACCTCCTATGGATTGCACCAAAAGGAACAACAGAAGCACC
  GTATTTCGAATAGTGCCAAAGTTTAAAAAGGAAAAGGTTCAGAAGCAGAAGACAAGTTCACAGCCTGTT
  CAGAAATGGGGCACAGAGGAAGTTGCTGCTTGGCTGGATCTGCTCAATTTGGGAGAGTACAAAGATATC
  TTCATCCGTCATGACATCAGAGGGGCTGAACTTTTGCATCTGGAAAGGCGAGATCTTAAGGATCTGGGG
  ATACCGAAAGTGGGTCATGTGAAGCGAATTCTCCAGGGAATTAAAGAGCTTGGAAGGAGCACTCCACAG
  TCGGAGGTGTAA
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001204506
• Insert Size: 3255 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001204506.1
• RefSeq Size: 3956 bp
• RefSeq ORF: 3255 bp
• Locus ID: 160851
• UniProt ID: Q86XP1
• Cytogenetics: 13q14.11
• Protein Families: Druggable Genome
• Protein Pathways: Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• MW: 120.6 kDa
• Gene Summary: This gene encodes a member of the diacylglycerol kinase (DGK) enzyme family. Members of this family are involved in regulating intracellular concentrations of diacylglycerol and phosphatidic acid. Variation in this gene has been associated with bipolar disorder. Alternatively spliced transcript variants have been identified. [provided by RefSeq, Jul 2014]
  Transcript Variant: This variant (5) differs in the 5' UTR, lacks a portion of the 5' coding region, and contains an alternate exon in the 3' coding region, which results in a frameshift, compared to variant 1. It initiates translation at a downstream in-frame start codon. The encoded isoform (4) has a shorter N-terminus and a distinct C-terminus, and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.