PCDH7 (NM_001173523) Human Untagged Clone

CAT#: SC329321

PCDH7 (untagged)-Human protocadherin 7 (PCDH7) transcript variant d

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PCDH7
• Synonyms: BH-Pcdh; BHPCDH; PPP1R120
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC329321 representing NM_001173523.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCTGAGGATGCGGACCGCGGGATGGGCGCGCGGCTGGTGCTTGGGCTGCTGCCTCCTCCTGCCGCTC
  TCGCTCAGCCTGGCGGCCGCCAAGCAGCTCCTCCGGTACCGGCTGGCCGAGGAGGGCCCCGCCGACGTC
  CGCATCGGCAACGTGGCTTCAGACCTGGGCATCGTGACCGGATCGGGTGAGGTGACTTTCAGCCTGGAG
  TCCGGTTCCGAGTACCTGAAGATCGACAACCTCACTGGCGAGCTGAGCACGAGCGAGCGGCGCATCGAC
  CGCGAGAAGCTGCCCCAGTGTCAGATGATCTTCGACGAGAACGAGTGCTTCCTGGACTTCGAGGTGTCG
  GTGATCGGGCCCTCGCAGAGCTGGGTGGACCTGTTTGAGGGTCAGGTCATCGTGCTTGACATCAACGAC
  AACACGCCCACCTTCCCGTCGCCCGTGCTCACGCTCACGGTGGAGGAGAATCGGCCGGTGGGCACACTT
  TACCTGCTGCCCACAGCCACCGACCGCGACTTCGGCCGCAACGGCATCGAGCGCTACGAGCTGCTCCAG
  GAGCCCGGAGGCGGCGGCAGCGGCGGCGAGAGCCGGCGCGCCGGGGCGGCCGACAGCGCCCCCTACCCC
  GGGGGCGGCGGGAACGGCGCGAGCGGCGGCGGCTCGGGAGGCTCCAAGCGGCGGCTGGACGCATCAGAG
  GGCGGCGGCGGCACCAACCCCGGCGGCCGCAGCAGCGTGTTCGAGCTGCAGGTGGCGGACACCCCGGAC
  GGCGAGAAGCAGCCGCAGCTGATCGTGAAGGGGGCGCTGGACCGCGAGCAGCGCGACTCCTACGAGCTG
  ACCCTGCGAGTGCGCGACGGCGGCGACCCGCCTCGCTCCTCGCAGGCCATCCTACGGGTCCTCATCACC
  GACGTGAACGACAACAGCCCCCGCTTCGAGAAGAGCGTGTACGAGGCCGACTTGGCTGAGAACAGCGCC
  CCGGGGACCCCCATCCTGCAACTGCGCGCAGCCGACTTGGACGTGGGGGTCAACGGGCAGATCGAATAC
  GTGTTCGGGGCGGCCACCGAGTCGGTGAGGCGGCTGCTGCGCCTTGACGAGACGTCCGGCTGGCTCAGC
  GTCCTGCACCGGATCGACCGCGAGGAGGTGAACCAGCTGCGCTTCACGGTCATGGCCCGCGACCGCGGG
  CAGCCCCCCAAGACCGACAAGGCCACCGTGGTCCTTAACATCAAAGACGAGAACGACAACGTGCCGTCC
  ATTGAAATCCGCAAGATTGGGCGCATCCCCCTCAAGGACGGGGTGGCCAACGTGGCCGAGGACGTTCTG
  GTCGACACCCCCATCGCTCTGGTGCAGGTGTCCGACCGAGACCAAGGCGAGAACGGGGTGGTCACCTGC
  ACCGTGGTGGGCGACGTGCCCTTCCAGCTCAAGCCAGCCAGCGACACCGAGGGCGACCAGAACAAGAAA
  AAGTACTTCTTGCACACCTCGACCCCTCTGGACTATGAGGCCACCCGGGAGTTCAACGTGGTCATCGTG
  GCGGTGGACTCAGGCAGCCCCAGCCTCTCGAGCAACAACTCCCTGATTGTCAAGGTGGGAGACACCAAC
  GACAACCCGCCCATGTTCGGCCAGTCGGTGGTGGAGGTTTACTTCCCTGAGAACAACATCCCGGGCGAG
  AGGGTGGCCACGGTGCTGGCGACAGACGCAGACAGCGGTAAGAACGCCGAGATCGCCTACTCGCTGGAC
  TCCTCTGTGATGGGGATCTTTGCCATCGATCCCGATTCTGGGGACATCCTGGTCAATACCGTGCTGGAC
  CGCGAGCAGACTGACAGGTATGAGTTTAAAGTTAACGCCAAAGACAAAGGCATCCCCGTGCTGCAGGGC
  AGCACTACGGTGATTGTGCAGGTGGCTGATAAAAATGACAATGACCCTAAGTTTATGCAGGACGTCTTC
  ACCTTTTATGTGAAAGAAAACTTGCAGCCCAACAGCCCTGTGGGGATGGTCACCGTGATGGATGCTGAC
  AAGGGGCGGAATGCAGAGATGAGCCTGTACATAGAGGAGAACAATAACATTTTTTCTATTGAAAATGAC
  ACGGGGACCATTTACTCCACAATGTCTTTTGACCGGGAACATCAGACCACATACACTTTCAGAGTCAAG
  GCTGTGGATGGGGGAGATCCTCCCAGATCTGCCACAGCTACAGTCTCGCTTTTTGTGATGGATGAAAAT
  GACAATGCTCCCACAGTTACCCTTCCCAAAAACATTTCCTACACTTTACTGCCACCTTCGAGTAATGTC
  AGGACAGTAGTAGCTACAGTGTTGGCAACAGACAGTGATGATGGCATCAATGCAGACCTGAACTACAGC
  ATTGTGGGAGGAAATCCCTTCAAGCTGTTTGAAATTGATCCCACTAGTGGTGTGGTTTCCTTAGTGGGA
  AAACTCACCCAAAAGCATTATGGCTTGCACAGGTTGGTGGTGCAAGTGAATGACAGTGGGCAGCCTTCC
  CAGTCCACCACGACTCTGGTGCACGTGTTTGTCAATGAAAGTGTTTCTAATGCAACTGCGATTGACTCC
  CAGATAGCTAGAAGTTTGCACATCCCACTCACCCAGGATATAGCTGGTGACCCAAGCTATGAAATTAGC
  AAACAGAGACTCAGTATTGTCATTGGCGTGGTTGCTGGCATTATGACGGTGATTCTAATCATCTTAATT
  GTAGTGATGGCAAGGTACTGCAGGTCCAAAAATAAAAATGGCTATGAAGCCGGCAAAAAAGATCACGAA
  GACTTTTTTACACCCCAACAGCATGACAAATCTAAAAAGCCTAAAAAGGACAAGAAAAACAAAAAATCT
  AAGCAGCCTCTCTACAGCAGCATTGTCACTGTGGAGGCTTCTAAGCCAAATGGACAGAGGTATGATAGT
  GTCAATGAGAAGCTGTCAGACAGCCCAAGCATGGGGCGATACAGGTCCGTTAATGGTGGGCCCGGCAGT
  CCTGACCTGGCAAGGCATTACAAATCTAGTTCCCCATTGCCTACTGTTCAGCTTCATCCCCAGTCACCA
  ACTGCAGGAAAAAAACACCAGGCCGTACAAGATCTACCACCAGCCAACACATTTGTGGGAGCAGGAGAC
  AACATTTCAATTGGATCAGATCACTGCTCTGAGTACAGCTGTCAAACCAATAACAAGTACAGCAAACAG
  CCATTTCGTAGAGTGACGTTTTCTGTTGTGAGTCAGCCTCAGGACCCACATCAGGGGTCACTGCAGAGT
  TGCTATGACAGCGGGCTGGAGGAGTCAGAAACACCAAGCAGTAAGAGTTCATCAGGGCCAAGACTGGGT
  GCGCTTCCACTCCCAGAGGACAACTATGAAAGGACCACGCCGGATGGCAGTGTTGGTGAGGCAGAGCAT
  ATGGAAAATGATTCAAGGCCTCTTCCAGATGTAGCCCTGACTGGGAAGTGCACTCGTGAGTGTGATGAG
  TATGGCCACTCAGACTCCTGCTGGATGCCGGTCCGCACTTCTCCGGAGAGGAAGAAGAGCCAGCCTAAA
  CTGTCCACTTTCATGCCTGTTGATGAACGAGGAAGCCAGGAAAAGCTGGCCAATGGGGAGGCCGCCATC
  ATGGGTGACCGCAACAGAAACCTCCTGAACAAAAAGTTGACCTCATCCTATGAGACCTTCAGTGCAGCT
  AGTTTCAGCAAAAATGAGGAAGCCAACCCTGAGGATATTCCCCTTACAAAAACAGGGGAATATAAGCCA
  TCTCCTGTCAATACTCTCACTAGAAGAGAAGTTTACCTGTAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001173523
• Insert Size: 3768 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001173523.1
• RefSeq Size: 8728 bp
• RefSeq ORF: 3768 bp
• Locus ID: 5099
• Cytogenetics: 4p15.1
• Protein Families: Druggable Genome, Transmembrane
• MW: 136.6 kDa
• Gene Summary: This gene belongs to the protocadherin gene family, a subfamily of the cadherin superfamily. The gene encodes a protein with an extracellular domain containing 7 cadherin repeats. The gene product is an integral membrane protein that is thought to function in cell-cell recognition and adhesion. Alternative splicing yields isoforms with unique cytoplasmic tails. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (d) represents the longest transcript and encodes the longest isoform (d). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.