PHKA1 (NM_001172436) Human Untagged Clone

CAT#: SC329301

PHKA1 (untagged)-Human phosphorylase kinase alpha 1 (muscle) (PHKA1) transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PHKA1
• Synonyms: PHKA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001172436, the custom clone sequence may differ by one or more nucleotides
  ATGAGGAGCCGGAGTAACTCCGGGGTCCGGCTGGACGGCTACGCTCGACTGGTGCAACAG
  ACCATCCTGTGCCATCAGAATCCAGTGACTGGCTTGCTTCCAGCCAGCTATGATCAGAAA
  GATGCTTGGGTCCGAGATAATGTGTACAGCATCTTGGCTGTGTGGGGTTTGGGCCTGGCC
  TATCGGAAGAATGCAGACCGGGATGAGGATAAGGCAAAGGCCTATGAATTGGAGCAGAGT
  GTAGTGAAGCTGATGAGAGGACTACTGCACTGCATGATCAGACAGGTGGATAAAGTAGAA
  TCCTTCAAATATAGTCAGAGTACTAAGGATAGCCTCCATGCAAAGTACAACACCAAAACC
  TGTGCCACTGTAGTGGGTGATGATCAATGGGGACACCTGCAGTTGGATGCTACCTCTGTG
  TACCTGCTCTTCTTAGCCCAAATGACTGCCTCAGGACTCCATATCATCCACAGCCTAGAT
  GAAGTCAATTTCATACAGAACCTTGTGTTTTACATTGAAGCTGCATATAAAACTGCTGAC
  TTCGGGATATGGGAACGTGGAGACAAGACCAACCAAGGGATCTCAGAGTTGAATGCCAGT
  TCAGTTGGAATGGCAAAGGCAGCCCTGGAAGCATTAGATGAACTGGATCTGTTTGGTGTG
  AAAGGTGGGCCTCAATCAGTTATCCATGTCCTGGCTGATGAAGTACAGCACTGCCAGTCT
  ATCCTAAATTCACTACTGCCCCGTGCTTCAACATCAAAAGAGGTTGATGCTAGTCTACTC
  TCAGTGGTTTCCTTCCCTGCCTTTGCAGTAGAGGATAGCCAGTTGGTGGAGCTCACAAAA
  CAGGAAATCATCACCAAGCTTCAGGGTCGTTATGGTTGCTGTCGCTTTCTACGAGATGGA
  TATAAAACTCCTAAAGAGGATCCCAATCGTCTGTACTATGAACCAGCTGAGCTGAAGCTA
  TTTGAAAACATTGAGTGTGAATGGCCATTGTTCTGGACATACTTTATTCTTGATGGGGTC
  TTCAGTGGCAATGCAGAACAGGTTCAAGAATATAAAGAGGCTCTTGAAGCAGTCCTCATC
  AAGGGCAAAAATGGAGTCCCACTTCTGCCAGAGCTGTACAGTGTTCCTCCTGACAGGGTC
  GATGAAGAATATCAGAATCCTCACACTGTGGACCGAGTCCCCATGGGGAAATTGCCTCAC
  ATGTGGGGTCAGTCTCTATACATTTTAGGAAGCTTGATGGCAGAGGGATTTTTAGCCCCT
  GGAGAAATTGATCCCCTGAATCGCAGGTTTTCTACTGTACCGAAGCCCGATGTTGTGGTT
  CAAGTCTCCATTCTAGCTGAAACAGAAGAAATCAAGACCATTTTGAAGGACAAGGGAATT
  TACGTGGAGACCATTGCTGAGGTATACCCCATCAGAGTACAACCAGCTCGTATTCTCAGC
  CACATTTATTCCAGCCTAGGATGCAACAATAGAATGAAACTCAGTGGACGACCCTACAGA
  CACATGGGAGTGCTTGGAACTTCAAAACTCTATGACATTCGGAAAACTATCTTTACTTTC
  ACTCCACAGTTTATAGACCAGCAACAGTTCTACCTGGCTCTGGACAACAAGATGATAGTG
  GAAATGCTTAGAACAGACCTCTCCTACCTCTGTAGCCGCTGGCGGATGACAGGCCAGCCC
  ACCATCACCTTCCCCATCTCACACAGCATGCTTGATGAAGATGGAACAAGCTTGAATTCA
  AGTATCCTGGCAGCACTCCGAAAAATGCAAGATGGGTATTTTGGTGGGGCAAGGGTTCAA
  ACAGGTAAATTGTCAGAGTTTTTGACAACATCTTGTTGCACACACTTGAGCTTCATGGAC
  CCTGGACCTGAGGGTAAGCTGTACAGTGAAGATTATGATGACAACTATGATTACCTGGAA
  TCTGGCAACTGGATGAATGATTATGATTCAACCAGTCATGATGTTCACATGTATCTTCCT
  ACGAAGTTATTTCAGGCTTCCCGGCCTTCATTCAACTTACTTGATTCACCTCATCCCCGA
  CAGGAGAACCAGGTTCCCTCTGTTCGTGTAGAAATACATCTTCCTAGAGACCAGTCTGGG
  GAGGTGGACTTTAAAGCACTGGTTTTACAGTTGAAGGAGACCTCAAGCTTACAGGAACAA
  GCTGATATCCTCTATATGCTGTATACTATGAAAGGACCTGACTGGAACACTGAATTGTAT
  AATGAACGGAGTGCTACAGTGAGAGAGCTTCTTACCGAGCTGTATGGCAAAGTGGGAGAA
  ATTCGTCACTGGGGCCTGATCCGATACATTTCTGGGATCTTAAGGAAGAAAGTGGAAGCA
  CTTGATGAGGCCTGCACAGACCTTCTCTCCCACCAGAAACATTTGACAGTAGGACTTCCT
  CCAGAACCTCGAGAAAAGACTATCTCTGCACCTCTGCCCTATGAGGCGCTCACTCAGCTG
  ATAGATGAAGCCAGTGAAGGGGATATGAGCATTTCAATCCTTACACAGGAAATAATGGTA
  TATCTAGCCATGTATATGCGAACCCAGCCTGGCCTCTTTGCTGAAATGTTTCGACTTCGA
  ATTGGTCTGATCATACAAGTTATGGCAACAGAACTGGCCCACTCCCTTCGATGCTCAGCT
  GAGGAAGCCACAGAGGGCCTGATGAATCTCAGTCCTTCGGCCATGAAGAATCTCCTGCAT
  CACATTCTCAGCGGCAAGGAGTTTGGAGTGGAACGAAGCGTTCGTCCCACTGATTCAAAT
  GTCAGTCCTGCTATTTCTATCCACGAGATTGGTGCTGTTGGAGCAACCAAAACAGAACGA
  ACTGGGATCATGCAGTTAAAAAGTGAGATAAAGCAGTCACCTGGAACCTCTATGACTCCA
  AGTAGTGGGTCCTTTCCTAGTGCATATGATCAGCAGTCATCTAAAGATAGTCGTCAAGGT
  CAATGGCAACGCCGAAGAAGGCTGGATGGGGCACTGAATAGAGTTCCAGTTGGATTTTAT
  CAGAAAGTATGGAAAGTTTTGCAGAAGTGTCACGGACTTTCTGTTGAAGGGTTTGTCCTT
  CCTTCCTCTACCACTAGAGAGATGACTCCAGGTGAGATTAAATTCTCTGTTCATGTGGAG
  TCTGTCCTGAATCGTGTACCTCAGCCAGAGTACCGTCAGCTGCTGGTTGAAGCCATCCTT
  GTCCTCACCATGCTGGCAGATATTGAAATTCATAGCATCGGAAGCATCATTGCTGTGGAA
  AAAATAGTGCATATTGCCAATGACTTGTTCCTTCAAGAACAGAAAACCCTTGGCGCAGAT
  GATACCATGTTGGCAAAGGATCCCGCATCTGGCATCTGTACTCTTCTGTATGACAGTGCA
  CCCAGTGGCAGGTTTGGCACCATGACCTACCTCTCCAAGGCAGCCGCCACCTACGTGCAG
  GAGTTCCTGCCCCACAGCATCTGTGCCATGCAATGA
  
• Restriction Sites: Please inquire
• ACCN: NM_001172436
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001172436.1, NP_001165907.1
• RefSeq Size: 5961 bp
• RefSeq ORF: 3456 bp
• Locus ID: 5255
• UniProt ID: P46020
• Cytogenetics: Xq13.1
• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway, Insulin signaling pathway
• Gene Summary: Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, and the skeletal muscle isoform is encoded by this gene. The beta subunit is the same in both the muscle and hepatic isoforms, and encoded by one gene. The gamma subunit also includes the skeletal muscle and hepatic isoforms, which are encoded by two different genes. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9D, also known as X-linked muscle glycogenosis. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene. A pseudogene has been found on chromosome 1.[provided by RefSeq, Feb 2010]
  Transcript Variant: This variant (3) lacks two alternate in-frame exons in the middle and 3' coding regions, respectively, compared to variant 1, resulting in a shorter protein (isoform 3), compared to isoform 1.