LAS1L (NM_001170649) Human Untagged Clone

CAT#: SC329093

LAS1L (untagged)-Human LAS1-like (S. cerevisiae) (LAS1L) transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LAS1L
• Synonyms: dJ475B7.2; Las1; Las1-like; WTS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001170649, the custom clone sequence may differ by one or more nucleotides
  ATGTCGTGGGAATCCGGGGCCGGGCCAGGTCTAGGTTCCCAGGGGATGGATCTCGTGTGG
  AGTGCGTGGTACGGAAAGTGCGTTAAAGGGAAAGGGTCGTTGCCACTCTCGGCCCACGGC
  ATCGTGGTCGCCTGGCTCAGCAGGGCCGAGTGGGACCAGGTGACGGTTTATCTGTTCTGT
  GACGACCATAAGTTGCAGCGGTACGCGCTTAACCGCATCACGGTGTGGAGGAGCAGGTCA
  GGCAACGAACTCCCTCTGGCAGTGGCTTCTACTGCTGACCTGATACGCTGTAAGCTCTTG
  GATGTAACTGGTGGCTTGGGCACTGATGAACTTAGACTGCTCTATGGCATGGCATTGGTC
  AGGTTTGTGAATCTTATCTCAGAGAGGAAGACAAAGTTTGCCAAGGTCCCCCTCAAGTGT
  CTGGCTCAAGAGGTAAATATTCCGGATTGGATTGTTGACCTTCGCCATGAGTTGACCCAC
  AAGAAAATGCCCCATATAAATGACTGCCGCAGAGGCTGCTACTTTGTCCTGGATTGGCTC
  CAGAAGACCTATTGGTGCCGCCAACTGGAGAACAGCCTGAGAGAGACCTGGGAGTTGGAG
  GAGTTCAGGGAAGGGATAGAGGAAGAGGATCAAGAGGAAGATAAGAACATTGTTGTTGAT
  GACATCACAGAACAGAAACCAGAGCCTCAGGATGATGGGAAAAGTACGGAGTCAGATGTA
  AAGGCCGATGGAGACAGCAAAGGCAGCGAAGAGGTGGATTCTCATTGCAAAAAGGCCCTG
  AGTCATAAAGAGCTATATGAAAGAGCCCGAGAACTGCTGGTATCATACGAAGAGGAGCAG
  TTTACGGTGCTGGAGAAATTTAGGTATTTACCTAAGGCCATTAAGGCGTGGAATAACCCG
  TCCCCACGTGTAGAATGTGTCCTGGCAGAGCTCAAGGGCGTTACATGCGAGAACAGGGAG
  GCTGTGCTGGATGCTTTTCTGGATGATGGCTTCCTTGTCCCCACATTTGAACAGTTGGCA
  GCTTTGCAGATAGAATATGAAGAAAACGTGGACTTGAATGACGTCCTGGTGCCAAAGCCG
  TTCTCTCAGTTCTGGCAGCCCCTGCTCAGGGGCCTGCACTCCCAGAACTTCACGCAGGCC
  CTATTGGAGAGGATGCTCTCTGAACTGCCAGCCTTGGGGATCAGCGGGATCCGGCCTACC
  TACATCCTCAGATGGACCGTTGAACTGATCGTGGCCAACACCAAGACTGGACGGAATGCT
  CGCCGATTTTCTGCAGGCCAGTGGGAAGCAAGAAGGGGCTGGAGGCTGTTCAACTGCTCC
  GCCTCCCTTGACTGGCCCCGGATGGTTGAGTCCTGCTTGGGCTCACCTTGCTGGGCCAGC
  CCCCAACTCCTTCGGATCATCTTCAAAGCCATGGGGCAGGGCCTGCCAGACGAGGAGCAG
  GAGAAGCTGCTGCGCATCTGTTCCATTTATACCCAGAGTGGAGAAAACAGCCTGGTGCAG
  GAGGGCTCTGAGGCCTCCCCCATTGGGAAGTCTCCATATACACTAGACAGCCTGTATTGG
  AGCGTCAAGCCAGCCAGCTCCAGCTTCGGGTCTGAAGCAAAGGCCCAGCAACAGGAGGAG
  CAGGGCAGTGTTAATGATGTCAAGGAAGAGGAGAAGGAGGAGAAAGAGGTCTTGCCAGAC
  CAGGTAGAGGAGGAGGAAGAAAATGATGACCAAGAGGAGGAAGAGGAGGATGAAGATGAT
  GAAGATGATGAAGAGGAAGACAGAATGGAGGTGGGGCCTTTCTCTACAGGGCAAGAGTCC
  CCCACTGCCGAGAATGCTAGGCTTCTGGCCCAGAAAAGAGGAGCTTTGCAGGGCTCTGCA
  TGGCAGGTTAGCTCAGAAGACGTGCGATGGGACACATTTCCCCTAGGCCGAATGCCAGGT
  CAGACCGAGGACCCAGCAGAGCTCATGCTGGAGAATTATGACACCATGTATCTTTTGGAC
  CAGCCTGTGCTAGAGCAGCGGCTGGAACCCTCAACATGCAAGACTGACACCTTGGGCCTG
  AGCTGTGGTGTCGGCAGTGGCAACTGCAGCAACAGCAGCAGCAGCAACTTCGAGGGCCTT
  CTCTGGAGCCAGGGGCAGCTGCATGGGCTCAAAACTGGCCTGCAGCTCTTCTGA
  
• Restriction Sites: Please inquire
• ACCN: NM_001170649
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001170649.1, NP_001164120.1
• RefSeq Size: 2448 bp
• RefSeq ORF: 2154 bp
• Locus ID: 81887
• UniProt ID: Q9Y4W2
• Cytogenetics: Xq12
• Gene Summary: Involved in the biogenesis of the 60S ribosomal subunit. Required for maturation of the 28S rRNA. Functions as a component of the Five Friends of Methylated CHTOP (5FMC) complex; the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the middle portion of the coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.