SCMH1 (NM_001172220) Human Untagged Clone

CAT#: SC328941

SCMH1 (untagged)-Human sex comb on midleg homolog 1 (Drosophila) (SCMH1) transcript variant 5

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SCMH1
• Synonyms: Scml3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC328941 representing NM_001172220.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAAATTGGAAGCACAGGACCCCAGGAACACCACATCCACCTGTATTGCCACAGTAGTTGGACTGACA
  GGTGCCCGCCTTCGCCTGCGCCTTGATGGGAGCGACAACAAAAATGACTTCTGGCGGCTGGTTGACTCA
  GCTGAAATCCAGCCTATTGGGAACTGTGAAAAGAATGGGGGTATGCTACAGCCACCTCTTGGATTTCGG
  CTGAATGCGTCTTCTTGGCCCATGTTCCTTTTGAAGACGCTAAATGGAGCAGAGATGGCTCCCATCAGG
  ATTTTCCACAAGGAGCCACCATCGCCTTCCCACAACTTCTTCAAAATGGGAATGAAGCTAGAAGCTGTG
  GACAGGAAGAACCCTCATTTCATTTGCCCAGCCACTATTGGGGAGGTTCGGGGCTCAGAGGTGCTTGTC
  ACTTTTGATGGGTGGCGAGGGGCCTTTGACTACTGGTGCCGCTTCGACTCCCGAGACATCTTCCCTGTG
  GGCTGGTGTTCCTTGACTGGAGACAACCTGCAGCCTCCTGGCACCAAAGTTGTGATTCCAAAGAATCCC
  TATCCTGCCTCCGATGTGAATACTGAGAAGCCCAGCATCCACAGCAGCACCAAAACTGTCTTGGAACAT
  CAACCAGGGCAGAGGGGGCGTAAACCAGGAAAGAAGCGGGGCCGGACACCCAAGACCCTAATTTCCCAT
  CCCATCTCTGCCCCATCCAAGACAGCTGAACCTTTGAAATTCCCAAAGAAGAGAGGTCCCAAACCTGGC
  AGCAAGAGGAAACCTCGGACTTTGCTGAACCCACCACCTGCCTCACCAACAACCAGCACTCCTGAACCG
  GATACCAGCACTGTACCCCAGGATGCTGCCACCATCCCCAGCTCAGCCATGCAGGCCCCAACAGTTTGT
  ATCTACTTGAACAAGAATGGCAGCACAGGCCCCCACTTAGATAAGAAGAAGGTCCAGCAACTCCCTGAC
  CATTTTGGACCAGCCCGTGCCTCTGTGGTGTTGCAGCAGGCTGTCCAGGCCTGTATCGACTGTGCTTAT
  CACCAGAAAACCGTCTTCAGCTTCCTCAAGCAAGGCCATGGTGGTGAGGTTATCTCAGCCGTGTTTGAC
  CGGGAACAGCATACCCTCAACCTCCCAGCAGTCAACAGCATCACCTACGTCCTCCGCTTCCTGGAGAAA
  CTCTGCCACAACCTTCGTAGTGACAATCTGTTTGGCAACCAGCCCTTTACACAGACTCACTTGTCACTC
  ACTGCCATAGAGTACAGCCACAGCCACGACAGGTACCTACCAGGTGAAACCTTTGTCCTGGGGAATAGT
  CTGGCCCGCTCCTTGGAACCACACTCAGACTCAATGGACTCTGCCTCAAATCCCACCAACCTTGTCAGC
  ACCTCCCAAAGGCACCGGCCCTTGCTTTCATCCTGTGGCCTCCCACCAAGCACTGCCTCAGCTGTGCGC
  AGGCTATGCTCCAGGGGGTCGGACCGATACCTGGAGAGCCGCGATGCCTCTCGACTGAGTGGCCGGGAC
  CCCTCCTCATGGACAGTCGAGGATGTGATGCAGTTTGTCCGGGAAGCTGATCCTCAGCTTGGACCCCAC
  GCTGACCTGTTTCGCAAACACGAGATCGATGGCAAGGCCCTGCTGCTGCTGCGCAGTGACATGATGATG
  AAGTACATGGGCCTGAAGCTGGGGCCTGCACTCAAGCTCTCCTACCACATTGACCGGCTGAAGCAGGGC
  AAGTTCTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001172220
• Insert Size: 1734 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001172220.1
• RefSeq Size: 3084 bp
• RefSeq ORF: 1734 bp
• Locus ID: 22955
• UniProt ID: Q96GD3
• Cytogenetics: 1p34.2
• Protein Families: Transcription Factors
• MW: 63.9 kDa
• Gene Summary: Associates with Polycomb group (PcG) multiprotein complexes; the complex class is required to maintain the transcriptionally repressive state of some genes.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (5) lacks two alternate exons in the 5' UTR, compared to variant 3. Both variants 3 and 5 encode the same isoform (c).