UBR2 (NM_001184801) Human Untagged Clone

CAT#: SC328723

UBR2 (untagged)-Human ubiquitin protein ligase E3 component n-recognin 2 (UBR2) transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: UBR2
• Synonyms: bA49A4.1; C6orf133; dJ242G1.1; dJ392M17.3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC328723 representing NM_001184801.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCGTCGGAGCTAGAGCCAGAGGTGCAGGCCATCGACCGGAGCTTGCTGGAATGTTCGGCCGAGGAG
  ATTGCGGGGAAATGGCTGCAAGCAACTGACCTCACTAGAGAAGTGTACCAGCATTTAGCCCACTATGTA
  CCCAAAATCTACTGCAGGGGTCCCAACCCTTTTCCACAGAAAGAAGACATGCTGGCACAGCATGTTTTG
  TTGGGACCAATGGAATGGTACCTTTGTGGTGAAGATCCTGCATTTGGATTTCCAAAACTTGAGCAAGCA
  AACAAACCTTCTCATCTTTGTGGTCGTGTTTTTAAAGTAGGAGAGCCTACATATTCTTGCAGAGACTGT
  GCAGTTGATCCAACTTGTGTTTTGTGCATGGAGTGCTTTTTGGGAAGTATTCACAGAGATCATCGATAT
  AGGATGACAACATCAGGAGGTGGAGGTTTCTGTGACTGTGGTGATACTGAAGCCTGGAAAGAGGGTCCT
  TACTGTCAAAAACATGAACTTAACACCTCTGAAATTGAGGAAGAAGAGGATCCTCTTGTTCATTTATCA
  GAAGATGTGATAGCAAGAACTTATAACATTTTTGCTATTACGTTTCGGTATGCAGTAGAAATATTAACC
  TGGGAAAAAGAAAGTGAATTGCCAGCAGATTTAGAGATGGTAGAGAAGAGTGACACCTACTATTGCATG
  CTGTTTAATGATGAGGTTCACACCTATGAACAAGTTATTTATACTCTTCAGAAAGCTGTTAACTGTACA
  CAAAAAGAAGCTATTGGTTTTGCAACTACAGTAGATCGAGATGGGCGTAGGTCTGTTCGATATGGAGAT
  TTTCAGTATTGTGAGCAAGCAAAATCAGTAATTGTGAGAAATACCAGTAGACAGACAAAGCCACTCAAA
  GTTCAAGTTATGCATTCGTCTATTGTCGCACATCAGAATTTTGGTTTGAAACTTTTGTCTTGGCTGGGA
  AGTATTATTGGATATTCAGATGGCCTTCGCCGGATTTTATGTCAAGTTGGTTTACAAGAAGGGCCAGAT
  GGTGAAAACTCTTCTCTAGTGGACAGACTGATGCTTAGTGATTCCAAATTATGGAAAGGTGCTAGGAGT
  GTATATCATCAGTTGTTCATGAGCAGTCTGCTTATGGATTTGAAATACAAGAAACTATTTGCTGTTCGA
  TTTGCAAAAAACTATGAGCGTTTGCAGAGTGATTATGTGACAGATGACCACGACAGAGAGTTTTCAGTC
  GCAGACCTCTCGGTTCAGATATTCACGGTTCCTTCACTTTTCTCTATCTCTGCTGGGCGCAGTGGCTCA
  CCTCTGTAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001184801
• Insert Size: 1320 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001184801.1
• RefSeq Size: 2493 bp
• RefSeq ORF: 1320 bp
• Locus ID: 23304
• UniProt ID: Q8IWV8
• Cytogenetics: 6p21.1
• Protein Families: Druggable Genome
• MW: 50 kDa
• Gene Summary: This gene encodes an E3 ubiquitin ligase of the N-end rule proteolytic pathway that targets proteins with destabilizing N-terminal residues for polyubiquitylation and proteasome-mediated degradation. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]
  Transcript Variant: This variant (2) differs in the 3' coding region and 3' UTR, compared to variant 1. The resulting protein (isoform 2) is shorter and has a distinct C-terminus, compared to isoform 1.