ATHL1 (PGGHG) (NM_025092) Human Untagged Clone

CAT#: SC327992

ATHL1 (untagged)-Human ATH1 acid trehalase-like 1 (yeast) (ATHL1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ATHL1
• Synonyms: ATHL1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC327992 representing NM_025092.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGAGGACGCCGGCGAGGACCCCACCACGTTTGCTGCCCACTCTCTGCCCAGTGACCCCCGTCTCTTG
  GCCACTGTGACCAACGCATACCTGGGCACACGAGTGTTTCACGACACGCTGCACGTGAGCGGCGTGTAC
  AATGGGGCTGGCGGGGACACGCACCGGGCCATGCTGCCCAGCCCCCTCAACGTCCGGCTGGAGGCCCCT
  GCAGGGATGGGGGAGCAGCTGACCGAGACCTTCGCCCTGGACACCAACACAGGCTCCTTTCTTCACACC
  CTGGAGGGCCCCCGCTTCCGGGCCTCCCAGTGCATCTATGCGCATCGCACGCTGCCCCACGTGCTGGCT
  TTCCGAGTGTCCATCGCCCGCCTGGCCCCGGGGAGCGGGCCCATCACGCTGCTCCTGCGGTCAGCCTTC
  TCCCCAGAAAGCCCAGACCTGGACCTGCATCAGGGTCCTGACTTCCAGGGAGCCCGGTACCTGTATGGC
  CACACCCTCACCCCTGAGCAGCCCGGGGGGCCACAGCAAGAGGTACACATGCTGTGGACACCAGCACCC
  CCAGACCTGACCCTTGGGGAAGGTGAGGAGGCTAGGACGTGGGACTTCCTGACAGCAGTGGGCGGCAGC
  CAGGCTGAGGCTCAGGCCTGCCTCACTGAGGCCCTGCAGCTGCAGGCCAGGGGAGCTCTGTATACGGCT
  CACGCACAGGCCTGGGCCCAGCTCTGGGTAGAATGTGGCTTGGACGTGGTGGGGCCCCTGCAGCTGCGC
  CAGGCCCTGCGTGGCTCCCTCTACTACCTGCTCAGTGCCCTGCCCCAGCCCAAGGCCCCAGGATACATC
  TGCCATGGCCTCAGTCCTGGGGGCCTCTCCAATGGGAGCCGTGAGGAATGCTACTGGGGCCACGTCTTC
  TGGGACCAGGACCTCTGGATGTTCCCGAGTATCCTGATGTTCCACCCAGAAGCCGCCAGGGCCATCCTG
  GAGTACCGCATCCGCACGCTGGACGGGGCCCTGGAGAACGCCCAGAACCTGGGCTACCAGGGAGCCAAG
  TTTGCCTGGGAGAGTGCAGACTCCGGCCTAGAGGTTTGCCCTGAGGACATTTACGGAGTCCAGGAGGTC
  CACGTCAACGGGGCCGTGGTGTTGGCCTTCGAGCTGTACTACCATACCACCCAGGACCTGCAGCTATTT
  CGAGAGGCTGGTGGCTGGGACGTGGTCAGGGCTGTGGCCGAGTTTTGGTGCAGTCGTGTTGAGTGGAGC
  CCCAGGGAGGAAAAGTACCACCTGAGGGGAGTCATGTCCCCCGACGAGTACCATTCAGGGGTCAACAAC
  TCTGTGTACACCAACGTCCTGGTCCAGAACAGCCTGCGCTTTGCTGCTGCCCTGGCCCAGGACCTGGGT
  CTTCCCATCCCCAGCCAGTGGCTGGCGGTGGCTGACAAGATCAAGGTACCCTTTGACGTGGAGCAGAAC
  TTCCACCCGGAGTTCGATGGGTATGAGCCTGGAGAGGTGGTGAAGCAGGCAGACGTCGTGCTCCTGGGA
  TACCCAGTCCCCTTCTCCCTGAGTCCTGATGTTCGCAGGAAAAATCTGGAGATTTACGAGGCTGTGACG
  TCCCCCCAGGGCCCCGCCATGACCTGGAGCATGTTTGCTGTGGGCTGGATGGAGCTGAAGGACGCAGTG
  CGGGCCCGGGGCCTCCTGGACAGGAGCTTTGCCAACATGGCTGAACCCTTCAAGGTGTGGACGGAGAAT
  GCAGACGGGTCAGGCGCTGTGAACTTCCTGACAGGCATGGGGGGCTTCCTGCAGGCGGTGGTCTTCGGG
  TGCACGGGGTTCAGGGTCACCCGAGCGGGTGTGACCTTTGACCCTGTGTGTCTGTCGGGGATCTCCAGA
  GTGAGCGTCTCCGGCATCTTCTACCAGGGGAACAAGCTCAACTTCTCTTTTTCCGAGGACTCCGTGACC
  GTGGAGGTCACAGCTCGAGCAGGGCCCTGGGCTCCTCACCTGGAGGCTGAGCTGTGGCCATCCCAGTCC
  CGGCTCTCCCTGTTGCCAGGACACAAGGTCTCCTTTCCCCGCTCGGCTGGCCGGATACAAATGTCACCC
  CCGAAGCTGCCTGGAAGTTCCAGCTCCGAGTTCCCTGGGAGGACTTTTTCAGATGTTAGGGACCCGCTC
  CAGAGCCCCCTCTGGGTCACCCTGGGTTCCTCCAGCCCCACCGAGTCACTCACTGTGGACCCTGCCTCT
  GAATAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_025092
• Insert Size: 2214 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025092.4
• RefSeq Size: 3270 bp
• RefSeq ORF: 2214 bp
• Locus ID: 80162
• UniProt ID: Q32M88
• Cytogenetics: 11p15.5
• Domains: Glyco_hydro_65m
• MW: 80.7 kDa
• Gene Summary: Catalyzes the hydrolysis of glucose from the disaccharide unit linked to hydroxylysine residues of collagen and collagen-like proteins.[UniProtKB/Swiss-Prot Function]