NUSAP1 (NM_016359) Human Untagged Clone

CAT#: SC327860

NUSAP1 (untagged)-Human nucleolar and spindle associated protein 1 (NUSAP1) transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NUSAP1
• Synonyms: ANKT; BM037; LNP; NUSAP; PRO0310p1; Q0310; SAPL
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_016359, the custom clone sequence may differ by one or more nucleotides
  ATGATCATCCCCTCTCTAGAGGAGCTGGACTCCCTCAAGTACAGTGACCTGCAGAACTTA
  GCCAAGAGTCTGGGTCTCCGGGCCAACCTGAGGGCAACCAAGTTGTTAAAAGCCTTGAAA
  GGCTACATTAAACATGAGGCAAGAAAAGGAAATGAGAATCAGGATGAAAGTCAAACTTCT
  GCATCCTCTTGTGATGAGACTGAGATACAGATCAGCAACCAGGAAGAAGCTGAGAGACAG
  CCACTTGGCCATGTCACCAAAACAAGGAGAAGGTGCAAGACTGTCCGTGTGGACCCTGAC
  TCACAGCAGAATCATTCAGAGATAAAAATAAGTAATCCCACTGAATTCCAGAATCATGAA
  AAGCAGGAAAGCCAGGATCTCAGAGCTACTGCAAAAGTTCCTTCTCCACCAGACGAGCAC
  CAAGAAGCTGAGAATGCTGTTTCCTCAGGTAACAGAGATTCAAAGGTACCTTCAGAAGGA
  AAGAAATCTCTCTACACAGATGAGTCATCCAAACCTGGAAAAAATAAAAGAACTGCAATC
  ACTACTCCAAACTTTAAGAAGCTTCATGAAGCTCATTTTAAGGAAATGGAGTCCATTGAT
  CAATATATTGAGAGAAAAAAGAAACATTTTGAAGAACACAATTCCATGAATGAACTGAAG
  CAGCAGCCCATCAATAAGGGAGGGGTCAGGACTCCAGTACCTCCAAGAGGAAGACTCTCT
  GTGGCTTCTACTCCCATCAGCCAACGACGCTCGCAAGGCCGGTCTTGTGGCCCTGCAAGT
  CAGAGTACCTTGGGTCTGAAGGGGTCACTCAAGCGCTCTGCTATCTCTGCAGCTAAAACG
  GGTGTCAGGTTTTCAGCTGCTACTAAAGATAATGAGCATAAGCGTTCACTGACCAAGACT
  CCAGCCAGAAAGTCTGCACATGTGACCGTGTCTGGGGGCACCCCAAAAGGCGAGGCTGTG
  CTTGGGACACACAAATTAAAGACCATCACGGGGAATTCTGCTGCTGTTATTACCCCATTC
  AAGTTGACAACTGAGGCAACGCAGACTCCAGTCTCCAATAAGAAACCAGTGTTTGATCTT
  AAAGCAAGTTTGTCTCGTCCCCTCAACTATGAACCACACAAAGGAAAGCTAAAACCATGG
  GGGCAATCTAAAGAAAATAATTATCTAAATCAACATGTCAACAGAATTAACTTCTACAAG
  AAAACTTACAAACAACCCCATCTCCAGACAAAGGAAGAGCAACGGAAGAAACGCGAGCAA
  GAACGAAAGGAGAAGAAAGCAAAGGTTTTGGGAATGCGAAGGGGCCTCATTTTGGCTGAA
  GAT
  
• Restriction Sites: Please inquire
• ACCN: NM_016359
• Insert Size: 2471 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_016359.3, NP_057443.2
• RefSeq Size: 2471 bp
• RefSeq ORF: 1326 bp
• Locus ID: 51203
• UniProt ID: Q9BXS6
• Cytogenetics: 15q15.1
• Gene Summary: NUSAP1 is a nucleolar-spindle-associated protein that plays a role in spindle microtubule organization (Raemaekers et al., 2003 [PubMed 12963707]).[supplied by OMIM, Jun 2009]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).