TJAP1 (NM_001146016) Human Untagged Clone

CAT#: SC327593

TJAP1 (untagged)-Human tight junction associated protein 1 (peripheral) (TJAP1) transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TJAP1
• Synonyms: PILT; TJP4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001146016, the custom clone sequence may differ by one or more nucleotides
  ATGACTAGTGCCGCCCCTGCTAAGAAACCCTACCGTAAGGCACCACCAGAGCATCGGGAG
  CTGCGTTTGGAAATTCCTGGATCCCGGCTTGAGCAGGAGGAACCCCTGACTGATGCAGAA
  AGGATGAAGCTCTTACAGGAGGAGAATGAAGAGCTTCGCCGGCGCCTGGCCTCCGCCACC
  AGACGCACTGAGGCCCTGGAACGTGAGCTGGAAATTGGGCAGGACTGCCTGGAGCTGGAG
  CTGGGCCAGAGCCGCGAGGAGCTGGACAAATTTAAGGATAAGTTCCGCAGGCTGCAGAAC
  AGCTACACGGCTTCCCAGCGGACCAACCAGGAGTTGGAGGACAAGCTGCACACACTGGCC
  TCTCTTAGCCACAGCTGGATTTTTGCAATCAAGAAGGCTGAGATGGATAGGAAGACGCTG
  GACTGGGAGATTGTGGAGCTGACCAACAAGCTGCTGGATGCCAAGAACACCATCAACAAG
  CTGGAAGAGCTCAATGAGCGGTACCGGCTGGACTGCAACCTGGCTGTACAGCTCCTCAAG
  TGCAACAAGTCCCACTTCCGAAACCACAAGTTTGCCGATCTGCCCTGTGAGCTACAGGAC
  ATGGTTCGGAAACATTTGCACAGTGGTCAAGAGGCCGCCAGCCCAGGTCCTGCTCCCAGC
  CTAGCCCCAGGGGCTGTGGTGCCTACCTCAGTCATTGCCCGAGTGTTAGAGAAGCCGGAG
  TCTCTACTGCTCAATTCAGCCCAGTCAGGCAGCGCCGGGCGCCCCTTGGCTGAGGATGTC
  TTTGTGCATGTGGACATGAGTGAGGGTGTCCCAGGTGATCCAGCCAGTCCCCCGGCCCCT
  GGCAGCCCCACCCCACAACCCAATGGGGAGTGCCACTCTCTGGGTACTGCCAGGGGCTCC
  CCGGAGGAAGAGCTGCCCCTGCCAGCCTTTGAGAAGCTGAACCCCTACCCAACCCCGTCT
  CCACCACACCCACTGTATCCTGGCCGCAGGGTAATAGAGTTCTCTGAGGATAAGGTTCGG
  ATCCCCCGCAACAGCCCCCTGCCCAACTGCACTTACGCTACCCGCCAGGCCATTTCCCTG
  AGCCTGGTAGAGGAGGGGAGTGAGCGGGCCCGCCCCAGCCCAGTGCCCAGCACCCCTGCC
  TCAGCCCAGGCCTCACCCCACCACCAGCCCAGCCCAGCACCCCTAACACTCAGTGCCCCA
  GCTAGCTCTGCCAGCTCTGAAGAGGACCTGCTGGTCAGCTGGCAGCGGGCATTTGTGGAC
  CGTACTCCACCACCTGCTGCTGTGGCCCAGCGCACAGCCTTTGGACGCGATGCCCTCCCT
  GAGCTGCAGCGCCATTTTGCCCATAGCCCCGCTGACAGAGATGAGGTGGTCCAGGCACCT
  TCTGCCCGACCCGAAGAGAGTGAGCTTTTGCTACCCACAGAACCTGACTCTGGCTTTCCC
  AGGGAGGAAGAAGAGCTGAACCTGCCTATCAGTCCTGAGGAAGAGCGCCAGAGCCTGCTG
  CCCATTAACAGGGGCACAGAGGAGGGGCCAGGCACTTCCCACACCGAGGGCAGGGCCTGG
  CCACTCCCCAGCTCCAGTCGCCCCCAGCGCAGCCCCAAGAGGATGGGGGTTCACCACCTG
  CACCGCAAGGACAGCCTGACCCAGGCCCAGGAGCAGGGCAACCTGCTCAAC
  
• Restriction Sites: Please inquire
• ACCN: NM_001146016
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001146016.1, NP_001139488.1
• RefSeq Size: 2761 bp
• RefSeq ORF: 1674 bp
• Locus ID: 93643
• UniProt ID: Q5JTD0
• Cytogenetics: 6p21.1
• Protein Pathways: Tight junction
• Gene Summary: This gene encodes a tight junction-associated protein. Incorporation of the encoded protein into tight junctions occurs at a late stage of formation of the junctions. The encoded protein localizes to the Golgi and may function in vesicle trafficking. Alternatively spliced transcript variants have been described. A related pseudogene exists on the X chromosome. [provided by RefSeq, Mar 2009]
  Transcript Variant: This variant (1) encodes the longer isoform (a). Variants 1, 2, and 8-15 all encode the same isoform (a).