PROB1 (NM_001161546) Human Untagged Clone

CAT#: SC327264

PROB1 (untagged)-Human hypothetical protein LOC389333 (LOC389333)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PROB1
• Synonyms: C5orf65
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC327264 representing NM_001161546.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCTGACCGCGCTCGCCCCGCCAGCCCTGCCTGGGATCCCGAGGCAGCTGCCCACGGCCCCCGCGCGG
  CGCCAGGACTCCTCCGGTTCGTCAGGCTCCTACTACACGGCTCCAGGTTCTCCGGAGCCCCCGGACGTT
  GGGCCGGACGCGAAAGGCCCAGCGAATTGGCCCTGGGTGGCTCCTGGGCGGGGGGCGGGCGCGCAGCCT
  CGCCTGTCCGTCAGCGCCCAGAATAGCCGCCAGCGGCACGGGCCCGGCTCGGGTTTCCCGCGAGGCCCA
  GGTTCCGGCCCACGGCCACCCCAGCCCCAGCTGCGCACGCTGCCGTCGGGGGAGATGGAAGTCATCTTC
  GGCGTCGGACCCCTGTTCGGCTGCTCCGGCGCAGACGATCGCGAGGCGCAACAACAGTTCACGGAGCCG
  GCCTTCATCAGCCCCTTGCCGCCGGGGCCAGCGTCTCCCGCCGCGGTCCCACGCCAGTCCCAGGTCCCC
  GATGGTGGCTCCCGCTGGGCCACCTACCTAGAGCTGCGGCCCCGTGGGCCAAGTCCTGCCGCCCCAGCG
  CAGTTCGAGTGTGTGGAGGTGGCTCTGGAGGAGGGCGCCGCGCCCGCCAGGCCCCGGACAGTGCCCAAG
  CGTCAGATCGAGCTGCGCCCCCGGCCCCAGAGTCCCCCAAGGGCGGCCGGCGCGCCGCGCCCCCGGCTG
  CTCCTGCGCACCGGTTCCCTGGACGAGTCGCTGGGCCCCCTGCAGGCCGCCGCGGGCTTCGTGCAGACG
  GCGTTGGCCAGAAAACTGAGCCCCGAGGCCCCGGCCCCGAGCAGCGCCACCTTCGGGTCCACGGGGCGG
  TCGGAACCTGAGACCCGGGAAACGGCCCGCAGCACCCACGTGGTCCTGGAGAAGGCTAAGTCTCGGCCA
  CTTCGCGTACGAGATAATTCGGCCCCCGCCAAGGCCCCGAGGCCGTGGCCCAGCCTCCGCGAGCGCGCG
  ATTCGGCGCGACAAGCCTGCGCCCGGGACGGAGCCGCTGGGTCCTGTTAGTTCCAGCATCTTCCTTCAG
  TCAGAGGAGAAGATCCAGGAGGCGCGGAAGACTCGGTTCCCGCGAGAGGCGCCGGATCGAACTGTTCAG
  AGGGCACGGAGTCCGCCTTTTGAGTGTAGGATCCCCTCGGAGGTTCCGAGTAGGGCTGTGAGGCCAAGG
  AGCCCGTCCCCGCCACGGCAGACTCCAAATGGGGCTGTACGGGGTCCTCGCTGCCCGTCGCCCCAGAAC
  CTGTCCCCGTGGGATCGGACTACTCGGAGGGTGAGTAGCCCATTGTTCCCTGAAGCCTCCTCCGAGTGG
  GAAAATCAAAATCCTGCCGTCGAGGAAACTGTCAGCAGGAGAAGCCCTTCCCCTCCGATCCTTTCCCAG
  TGGAATCAGTGTGTTGCTGGGGAAAGGAGCCCGTCCCTCGAAGCCCCTTCCCTGTGGGAGATTCCACAT
  TCGGCAGTCGCGGATGCGGTGGAGCCACGTAGCAGCCCGTCCCCGCCGGCCTTCTTCCCGTGGGAGGCT
  CCAGATCGTCCTATTGGAACTTGGGGCCCATCTCCCCAAGAGACATGGGATCCCATGGGGCCGGGCTCA
  TCGATAGCATTTACTCAGGAAGCTCAGAATGGGTTAACTCAGGAGGAGTTGGCACCGCCTACACCGTCC
  GCACCCGGCACTCCAGAACCAACAGAGATGCAGAGTCCATCCACGCGGGAAATTTCAGATCTTGCCTTT
  GGAGGGAGTCAGCAGTCCCCAGAGGTAGCAGCACCCGAGCCGCCCGGCAGCCACCCTGTGGGCACCCTG
  GACGCGGATAAGTGCCCGGAAGTCTTGGGTCCTGGAGAGGCGGCCTCGGGACGCCCGCGCATGGCCATT
  CCGCGGCCTCGAGACGTGCGCAAGTTGGTGAAGACCACGTACGCGCCAGGCTTCCCGGCAGGAGCACAA
  GGCTCTGGGCTGCCTGCGCCTCCTGCCGACCCCTGCGGGGAGGAGGGCGGCGAATCCAAGACGCAAGAG
  CCGCCAGCACTGGGGCCCCCCGCCCCGGCTCACTACACTTCCGTTTTCATCAAGGATTTTCTACCGGTG
  GTGCCGCACCCCTACGAGCCTCCTGAACCGTCCTTCGACACGGTCGCCCGGGACGCCTCACAGCCCAAC
  GGGGTCCTGCGGCGGAGGGCAGAGAACAGCACGGCGAAGCCCTTCAAGCGCACAGAGATCCGCCTGCCT
  GGGGCCCTGGCCTTGGGTCGCCGGCCCGAGGTAACCTCGCGAGTGCGAGCGCGCGGCCCTGGAGGAGAG
  AACAGGGATGTAGAGGCCCAGCGCCTGGTCCCCGACGGCGACGGTCGGACCAGCCCTCTAGGCGGCGCC
  CGCAGCTCATCCCAGCGCTCCCCCGTAGGGCCAGCAGGGGTCCGATCGCCCCGCCCCGGCTCCCCTCAG
  ATGCAAGCCAGCCCGAGCCCTGGGATAGCACCCAAACCGAAGACACCACCTACGGCCCCCGAGCCCGCG
  GCTGCCGTCCAGGCGCCACTCCCGCGGGAGCCCCTGGCGTTGGCGGGCAGGACGGCCCCAGCCCAGCCC
  CGCGCTGCCTCGGCGCCTCCCACGGACCGGTCCCCGCAAAGCCCCTCCCAGGGAGCGCGCAGGCAGCCC
  GGGGCCGCGCCCCTGGGGAAGGTCTTGGTGGACCCCGAGAGCGGCCGCTACTACTTTGTGGAGGCGCCG
  CGGCAGCCTCGGCTGCGGGTGCTCTTCGACCCTGAGAGTGGGCAGTACGTGGAGGTGTTGCTGCCGCCC
  TCGTCTCCCGGGCCGCCCCACCGCGTCTACACCCCTCTGGCCCTGGGGCTCGGCCTCTACCCGCCCGCC
  TATGGGCCTATACCCAGCCTCTCTCTGCCACCGTCCCCGGGCCCGCAGGCCCTCGGCAGCCCCCAGCTA
  CCCTGGGTCTCTGAGGCAGGGCCCCTGGACGGGACCTACTACCTGCCGGTGAGCGGGACCCCCAACCCC
  GCACCTCCTCTGCTCCTCTGTGCGCCGCCCTCCAGCTCAGGTCCCACCCAGCCCGGCAAGGGTTCATTG
  TTCCCGCTGTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001161546
• Insert Size: 3048 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001161546.1
• RefSeq Size: 3251 bp
• RefSeq ORF: 3048 bp
• Locus ID: 389333
• UniProt ID: E7EW31
• Cytogenetics: 5q31.2
• MW: 106.9 kDa