BRD8 (NM_001164326) Human Untagged Clone

CAT#: SC327236

BRD8 (untagged)-Human bromodomain containing 8 (BRD8) transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BRD8
• Synonyms: p120; SMAP; SMAP2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001164326, the custom clone sequence may differ by one or more nucleotides
  ATGGCGACGGGAACGGGCAAACACAAGCTGCTAAGCACTGGCCCCACAGAGCCATGGTCC
  ATCCGAGAGAAGCTATGTTTAGCATCTTCTGTCATGAGAAGTGGCGATCAAAATTGGGTA
  TCAGTTAGCAGAGCAATCAAGCCCTTTGCAGAACCTGGCCGCCCTCCAGACTGGTTCTCT
  CAAAAACATTGTGCTTCCCAGTACTCGGAGCTTTTAGAGACCACTGAGACACCAAAACGG
  AAACGAGGTGAAAAGGGAGAAGTGGTGGAAACTGTTGAAGATGTTATTGTTCGGAAATTG
  ACTGCTGAGCGAGTTGAAGAACTAAAGAAAGTGATAAAGGAAACCCAGGAGAGATATAGA
  CGGCTAAAGAGAGATGCAGAACTAATTCAAGCTGGACACATGGACAGCAGACTGGATGAG
  CTTTGCAATGACATTGCAACGAAAAAGAAATTGGAAGAAGAGGAGGCTGAAGTAAAGAGG
  AAGGCTACAGATGCTGCATACCAGGCTCGTCAAGCAGTAAAAACACCCCCCCGGAGGTTA
  CCCACTGTGATGGTTCGCTCTCCTATAGATTCTGCCTCCCCAGGAGGTGATTATCCACTT
  GGGGACTTGACTCCAACCACTATGGAAGAGGCTACCTCTGGGGTAACCCCCGGGACTTTG
  CCGAGTACCCCAGTCACCTCGTTTCCTGGGATTCCTGACACCCTTCCTCCAGGCTCTGCA
  CCCTTAGAAGCCCCCATGACCCCAGTAACAGATGATTCACCCCAGAAAAAGATGCTTGGA
  CAGAAAGCAACTCCACCCCCCTCCCCTCTGCTGTCAGAGCTCTTGAAGAAGGGCAGCCTC
  CTGCCTACTAGCCCCAGACTGGTCAATGAGAGTGAAATGGCTGTGGCTTCTGGCCACCTG
  AACAGTACAGGTGTCCTCCTGGAGGTAGGCGGGGTCCTTCCCATGATACATGGTGGGGAG
  ATACAGCAAACACCCAATACTGTTGCAGCCTCCCCTGCTGCATCAGTGAGTCAACCCGAC
  AACTGTGTTCCCATGGAGGCTGTGGGGGATCCACATACTGTGACTGTTTCCATGGACAGC
  AGTGAAATATCCATGATCATCAATTCTATCAAAGAAGAGTGTTTTCGATCAGGGGTAGCA
  GAGGCTCCTGTTGGATCAAAGGCTCCCAGCATAGATGGGAAGGAAGAATTAGATCTGGCT
  GAGAAGATGGATATTGCTGTGTCTTACACAGGTGAAGAGCTGGATTTTGAGACTGTTGGA
  GACATCATTGCCATCATTGAGGACAAGGTAGATGATCATCCTGAAGTGCTGGATGTGGCA
  GCAGTGGAAGCAGCACTGTCATTTTGTGAAGAAAATGATGATCCTCAGTCCCTGCCTGGC
  CCCTGGGAGCATCCTATCCAGCAGGAGCGGGACAAGCCAGTACCTCTCCCTGCACCAGAA
  ATGACGGTCAAGCAAGAGAGACTGGACTTTGAGGAAACGGAAAACAAGGGAATACATGAA
  CTGGTGGACATCAGGGAGCCCAGTGCAGAGATCAAGGTGGAACCTGCAGAACCAGAGCCA
  GTCATTTCAGGAGCCGAAATAGTAGCTGGAGTTGTTCCAGCCACAAGTATGGAGCCACCA
  GAACTCAGGAGTCAGGACTTAGATGAGGAACTGGGAAGTACTGCAGCTGGAGAGATTGTT
  GAAGCAGATGTTGCCATTGGGAAAGGCGATGAGACTCCACTTACAAATGTGAAGACAGAG
  GCATCCCCTGAAAGCATGTTGTCTCCATCACATGGCTCAAATCCCATTGAAGATCCTTTA
  GAGGCAGAGACTCAGCACAAGTTTGAAATGTCAGACTCATTGAAAGAAGAATCAGGGACT
  ATTTTTGGAAGCCAGATAAAGGATGCCCCAGGTGAGGATGAGGAGGAAGATGGTGTCAGT
  GAAGCGGCCAGCCTAGAGGAGCCTAAGGAAGAGGATCAAGGAGAAGGCTACTTGTCAGAA
  ATGGATAATGAACCTCCTGTGAGCGAGAGTGATGATGGCTTCAGCATACACAATGCTACA
  CTGCAGTCACACACACTGGCAGACTCCATCCCCAGCAGCCCTGCTTCTTCACAGTTCTCT
  GTCTGTAGTGAGGATCAGGAAGCTATTCAGGCACAGAAAATTTGGAAGAAAGCCATCATG
  CTTGTATGGAGAGCTGCAGCTAATCATAGGTATGCCAATGTCTTCCTGCAGCCTGTTACA
  GATGACATAGCACCTGGCTACCACAGCATTGTGCAGAGGCCTATGGATTTGTCAACTATT
  AAGAAAAACATAGAAAATGGACTGATCCGAAGCACAGCTGAATTTCAGCGTGACATTATG
  CTGATGTTTCAGAATGCTGTAATGTACAATAGCTCAGACCATGATGTCTATCACATGGCA
  GTGGAGATGCAGCGAGATGTCTTGGAACAGATCCAGCAATTCTTGGCCACGCAGTTGATT
  ATGCAAACATCCGAGTCTGGGATCAGTGCTAAAAGTCTTCGAGGGAGAGATTCTACCCGC
  AAACAGGATGCTTCAGAGAAGGATGGAGGAACCAGGGGACGCCGCTGTGCCATTGAAGCA
  GATATGAAGATGAAAAAG
  
• Restriction Sites: Please inquire
• ACCN: NM_001164326
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164326.1, NP_001157798.1
• RefSeq Size: 2998 bp
• RefSeq ORF: 2601 bp
• Locus ID: 10902
• UniProt ID: Q9H0E9
• Cytogenetics: 5q31.2
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: The protein encoded by this gene interacts with thyroid hormone receptor in a ligand-dependent manner and enhances thyroid hormone-dependent activation from thyroid response elements. This protein contains a bromodomain and is thought to be a nuclear receptor coactivator. Multiple alternatively spliced transcript variants that encode distinct isoforms have been identified. [provided by RefSeq, Jul 2014]
  Transcript Variant: This variant (4) lacks two alternate in-frame exons compared to variant 1. The resulting isoform (4) has the same N- and C-termini but is shorter compared to isoform 1.