CASS4 (NM_001164115) Human Untagged Clone

CAT#: SC326913

CASS4 (untagged)-Human Cas scaffolding protein family member 4 (CASS4) transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CASS4
• Synonyms: C20orf32; CAS4; HEFL; HEPL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC326913 representing NM_001164115.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAAGGGAACAGGCATCATGGACTGTGCGCCCAAGGCACTCCTGGCCAGGGCACTTTATGACAACTGC
  CCTGACTGCTCTGACGAGCTGGCTTTCAGCAGAGGGGACATCCTGACCATTCTGGAGCAACACGTGCCA
  GAAAGCGAGGGTTGGTGGAAGTGTTTGCTCCATGGGAGGCAAGGCCTGGCCCCTGCCAACCGCCTCCAA
  ATCCTCACGGAGGTCGCTGCAGACAGGCCGTGCCCCCCATTCCTGAGAGGCCTGGAAGAAGCTCCTGCC
  AGCTCAGAGGAGACCTATCAGGTGCCCACTCTACCCCGCCCTCCCACTCCAGGCCCCGTTTATGAGCAG
  ATGAGGAGTTGGGCGGAGGGGCCCCAGCCCCCTACTGCCCAAGTCTATGAATTCCCCGACCCTCCCACC
  AGTGCCAGAATCATCTGTGAAAAGACTCTCAGCTTTCCAAAACAGGCCATCCTCACGCTTCCCAGACCT
  GTCCGGGCCTCACTGCCGACTCTGCCTTCCCAGGTGTATGACGTGCCTACCCAGCACCGGGGCCCCGTG
  GTCCTGAAGGAGCCAGAGAAGCAGCAGTTATATGACATACCAGCCAGCCCCAAGAAGGCAGGACTCCAT
  CCCCCAGACAGCCAAGCAAGTAATCCTGGCCCTCTTATACCTCAGCCTTCGAGTCAACAGACTCCTGAG
  AGGAAACCCCGCTTATCTGAACACTGCCGGCTCTACTTTGGGGCGCTCTTCAAAGCCATCAGCGCATTT
  CACGGCAGCCTCAGCAGCAGCCAGCCCGCGGAGATCATCACTCAGAGCAAGCTGGTCATCATGGTGGGA
  CAGAAGCTGGTGGACACGCTGTGCATGGAGACCCAGGAGAGGGACGTGCGCAACGAGATCCTCCGTGGC
  AGCAGTCACCTCTGCAGCCTGCTCAAGGACGTAGCGCTGGCCACTAAGAATGCCGTGCTCACGTACCCC
  AGCCCTGCCGCGCTGGGGCACCTCCAGGCGGAGGCTGAGAAGCTGGAGCAACACACGCGGCAGTTCAGA
  GGGACACTGGGATGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001164115
• Insert Size: 1050 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164115.1
• RefSeq Size: 1844 bp
• RefSeq ORF: 1050 bp
• Locus ID: 57091
• UniProt ID: Q9NQ75
• Cytogenetics: 20q13.31
• MW: 38.3 kDa
• Gene Summary: Docking protein that plays a role in tyrosine kinase-based signaling related to cell adhesion and cell spreading. Regulates PTK2/FAK1 activity, focal adhesion integrity, and cell spreading.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (c) has the same N- and C-termini but is shorter compared to isoform a.