RPAIN (NM_001160244) Human Untagged Clone

CAT#: SC326744

RPAIN (untagged)-Human RPA interacting protein (RPAIN) transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPAIN
• Synonyms: HRIP; RIP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001160244, the custom clone sequence may differ by one or more nucleotides
  ATGGCGGAGTCGTTGAGGTCTCCGCGCCGCTCCCTGTACAAACTGGTGGGCTCGCCGCCT
  TGGAAAGAGGCTTTCCGGCAGAGATGCCTGGAGAGAATGAGAAACAGCCGGGACAGGCTC
  CTAAACAGGTACCGCCAGGCTGGAAGCAGTGGGCCAGGGAATTCTCAGAACAGCTTTCTA
  GTTCAAGAGGTGATGGAAGAAGAGTGGAATGCTTTGCAGTCAGTGGAGAATTGTCCAGAA
  GACTTGGCTCAGCTGGAGGAGCTGATAGACATGGCTGTGCTGGAGGAAATTCAACAGGAG
  CTGATCAACCAAGAGCAGTCCATCATCAGCGAGTATGAGAAGAGCTTGCAGTTTGATGAA
  AAGTGTCTCAGCATCATGCTGGCTGAGTGGGAGGCAAACCCACTCATCTGTCCTGTATGT
  ACAAAGTACAACCTGAGAATCACAAGCGGTGTGGTGGTGTGTCAGTGTGGCCTGTCCATC
  CCATCTCATGCCTGTGATACTTGGGCTGTGATCCTC
  
• Restriction Sites: Please inquire
• ACCN: NM_001160244
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001160244.1, NP_001153716.1
• RefSeq Size: 1538 bp
• RefSeq ORF: 519 bp
• Locus ID: 84268
• UniProt ID: Q86UA6
• Cytogenetics: 17p13.2
• Gene Summary: Mediates the import of RPA complex into the nucleus, possibly via some interaction with importin beta. Isoform 2 is sumoylated and mediates the localization of RPA complex into the PML body of the nucleus, thereby participating in RPA function in DNA metabolism.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) differs in the 3' coding region and 3' UTR, compared to variant 1. It encodes isoform c, which has a shorter and distinct C-terminus compared to isoform a.