ROBO1 (NM_001145844) Human Untagged Clone

CAT#: SC326515

ROBO1 (untagged)-Human roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant 3, mRNA

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ROBO1
• Synonyms: axon guidance receptor; DUTT1; FLJ21882; MGC131599; MGC133277; roundabout, axon guidance receptor, homolog 1 (Drosophila); roundabout 1; SAX3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001145844 edited
  TCTGCCTCGGCTCTTTGTTGTTCGCTTTGGATGGTTCTTGAAAGTGTCTGAGCCTCCTCG
  GAAATCCTGGGGCCGGAGAAGACAAACCTTGGAATTCTTCCTCTGCAAAAGTCTCTGAGA
  TACTGACAAGCGTCCGGAAAGGTCGACGAGTAATTGCCCTGAAAACTCTTGGCTAATTGA
  CCCACGTTGCTTATATTAAGCCTTTGTGTGTGGTGTGTGGCTTCATACATTTGGGGACCC
  TATTTCCACTCCCTCCTCTTGGCATGAGACTGTATACAGGATCCACCCGAGGACAATGAT
  TGCGGAGCCCGCTCACTTTTACCTGTTTGGATTAATATGTCTCTGTTCAGGCTCCCGTCT
  TCGTCAGGAAGATTTTCCACCTCGCATTGTTGAACACCCTTCAGACCTGATTGTCTCAAA
  AGGAGAACCTGCAACTTTGAACTGCAAAGCTGAAGGCCGCCCCACACCCACTATTGAATG
  GTACAAAGGGGGAGAGAGAGTGGAGACAGACAAAGATGACCCTCGCTCACACCGAATGTT
  GCTGCCGAGTGGATCTTTATTTTTCTTACGTATAGTACATGGACGGAAAAGTAGACCTGA
  TGAAGGAGTCTATGTCTGTGTAGCAAGGAATTACCTTGGAGAGGCTGTGAGCCACAATGC
  ATCGCTGGAAGTAGCCATACTTCGGGATGACTTCAGACAAAACCCTTCGGATGTCATGGT
  TGCAGTAGGAGAGCCTGCAGTAATGGAATGCCAACCTCCACGAGGCCATCCTGAGCCCAC
  CATTTCATGGAAGAAAGATGGCTCTCCACTGGATGATAAAGATGAAAGAATAACTATACG
  AGGAGGAAAGCTCATGATCACTTACACCCGTAAAAGTGACGCTGGCAAATATGTTTGTGT
  TGGTACCAATATGGTTGGGGAACGTGAGAGTGAAGTAGCCGAGCTGACTGTCTTAGAGAG
  ACCATCATTTGTGAAGAGACCCAGTAACTTGGCAGTAACTGTGGATGACAGTGCAGAATT
  TAAATGTGAGGCCCGAGGTGACCCTGTACCTACAGTACGATGGAGGAAAGATGATGGAGA
  GCTGCCCAAATCCAGATATGAAATCCGAGATGATCATACCTTGAAAATTAGGAAGGTGAC
  AGCTGGTGACATGGGTTCATACACTTGTGTTGCAGAAAATATGGTGGGCAAAGCTGAAGC
  ATCTGCTACTCTGACTGTTCAAGTTGGGTCTGAACCTCCACATTTTGTTGTGAAACCCCG
  TGACCAGGTTGTTGCTTTGGGACGGACTGTAACTTTTCAGTGTGAAGCAACCGGAAATCC
  TCAACCAGCTATTTTCTGGAGGAGAGAAGGGAGTCAGAATCTACTTTTCTCATATCAACC
  ACCACAGTCATCCAGCCGATTTTCAGTCTCCCAGACTGGCGACCTCACAATTACTAATGT
  CCAGCGATCTGATGTTGGTTATTACATCTGCCAGACTTTAAATGTTGCTGGAAGCATCAT
  CACAAAGGCATATTTGGAAGTTACAGATGTGATTGCAGATCGGCCTCCCCCAGTTATTCG
  ACAAGGTCCTGTGAATCAGACTGTAGCCGTGGATGGCACTTTCGTCCTCAGCTGTGTGGC
  CACAGGCAGTCCAGTGCCCACCATTCTGTGGAGAAAGGATGGAGTCCTCGTTTCAACCCA
  AGACTCTCGAATCAAACAGTTGGAGAATGGAGTACTGCAGATCCGATATGCTAAGCTGGG
  TGATACTGGTCGGTACACCTGCATTGCATCAACCCCCAGTGGTGAAGCAACATGGAGTGC
  TTACATTGAAGTTCAAGAATTTGGAGTTCCAGTTCAGCCTCCAAGACCTACTGACCCAAA
  TTTAATCCCTAGTGCCCCATCAAAACCTGAAGTGACAGATGTCAGCAGAAATACAGTCAC
  ATTATCATGGCAACCAAATTTGAATTCAGGAGCAACTCCAACATCTTATATTATAGAAGC
  CTTCAGCCATGCATCTGGTAGCAGCTGGCAGACCGTAGCAGAGAATGTGAAAACAGAAAC
  ATCTGCCATTAAAGGACTCAAACCTAATGCAATTTACCTTTTCCTTGTGAGGGCAGCTAA
  TGCATATGGAATTAGTGATCCAAGCCAAATATCAGATCCAGTGAAAACACAAGATGTCCT
  ACCAACAAGTCAGGGGGTGGACCACAAGCAGGTCCAGAGAGAGCTGGGAAATGCTGTTCT
  GCACCTCCACAACCCCACCGTCCTTTCTTCCTCTTCCATCGAAGTGCACTGGACAGTAGA
  TCAACAGTCTCAGTATATACAAGGATATAAAATTCTCTATCGGCCATCTGGAGCCAACCA
  CGGAGAATCAGACTGGTTAGTTTTTGAAGTGAGGACGCCAGCCAAAAACAGTGTGGTAAT
  CCCTGATCTCAGAAAGGGAGTCAACTATGAAATTAAGGCTCGCCCTTTTTTTAATGAATT
  TCAAGGAGCAGATAGTGAAATCAAGTTTGCCAAAACCCTGGAAGAAGCACCCAGTGCCCC
  ACCCCAAGGTGTAACTGTATCCAAGAATGATGGAAACGGAACTGCAATTCTAGTTAGTTG
  GCAGCCACCTCCAGAAGACACTCAAAATGGAATGGTCCAAGAGTATAAGGTTTGGTGTCT
  GGGCAATGAAACTCGATACCACATCAACAAAACAGTGGATGGTTCCACCTTTTCCGTGGT
  CATTCCCTTTCTTGTTCCTGGAATCCGATACAGTGTGGAAGTGGCAGCCAGCACTGGGGC
  TGGGTCTGGGGTAAAGAGTGAGCCTCAGTTCATCCAGCTGGATGCCCATGGAAACCCTGT
  GTCACCTGAGGACCAAGTCAGCCTCGCTCAGCAGATTTCAGATGTGGTGAAGCAGCCGGC
  CTTCATAGCAGGTATTGGAGCAGCCTGTTGGATCATCCTCATGGTCTTCAGCATCTGGCT
  TTATCGACACCGCAAGAAGAGAAACGGACTTACTAGTACATACGCGGGTATCAGAAAAGT
  AACTTACCAGAGAGGAGGCGAAGCTGTCAGCAGTGGAGGGAGGCCTGGACTTCTCAACAT
  CAGTGAACCTGCCGCGCAGCCATGGCTGGCAGACACGTGGCCTAATACTGGCAACAACCA
  CAATGACTGCTCCATCAGCTGCTGCACGGCAGGCAATGGAAACAGCGACAGCAACCTCAC
  TACCTACAGTCGCCCAGCTGATTGTATAGCAAATTATAACAACCAACTGGATAACAAACA
  AACAAATCTGATGCTCCCTGAGTCAACTGTTTATGGTGATGTGGACCTTAGTAACAAAAT
  CAATGAGATGAAAACCTTCAATAGCCCAAATCTGAAGGATGGGCGTTTTGTCAATCCATC
  AGGGCAGCCTACTCCTTACGCCACCACTCAGCTCATCCAGTCAAACCTCAGCAACAACAT
  GAACAATGGCAGCGGGGACTCTGGCGAGAAGCACTGGAAACCACTGGGACAGCAGAAACA
  AGAAGTGGCACCAGTTCAGTACAACATCGTGGAGCAAAACAAGCTGAACAAAGATTATCG
  AGCAAATGACACAGTTCCTCCAACTATCCCATACAACCAATCATACGACCAGAACACAGG
  AGGATCCTACAACAGCTCAGACCGGGGCAGTAGTACATCTGGGAGTCAGGGGCACAAGAA
  AGGGGCAAGAACACCCAAGGTACCAAAACAGGGTGGCATGAACTGGGCAGACCTGCTTCC
  TCCTCCCCCAGCACATCCTCCTCCACACAGCAATAGCGAAGAGTACAACATTTCTGTAGA
  TGAAAGCTATGACCAAGAAATGCCATGTCCCGTGCCACCAGCAAGGATGTATTTGCAACA
  AGATGAATTAGAAGAGGAGGAAGATGAACGAGGCCCCACTCCCCCTGTTCGGGGAGCAGC
  TTCTTCTCCAGCTGCCGTGTCCTATAGCCATCAGTCCACTGCCACTCTGACTCCCTCCCC
  ACAGGAAGAACTCCAGCCCATGTTACAGGATTGTCCAGAGGAGACTGGCCACATGCAGCA
  CCAGCCCGACAGGAGACGGCAGCCTGTGAGTCCTCCTCCACCACCACGGCCGATCTCCCC
  TCCACATACCTATGGCTACATTTCAGGACCCCTGGTCTCAGATATGGATACGGATGCGCC
  AGAAGAGGAAGAAGACGAAGCCGACATGGAGGTAGCCAAGATGCAAACCAGAAGGCTTTT
  GTTACGTGGGCTTGAGCAGACACCTGCCTCCAGTGTTGGGGACCTGGAGAGCTCTGTCAC
  GGGGTCCATGATCAACGGCTGGGGCTCAGCCTCAGAGGAGGACAACATTTCCAGCGGACG
  CTCCAGTGTTAGTTCTTCGGACGGCTCCTTTTTCACTGATGCTGACTTTGCCCAGGCAGT
  CGCAGCAGCGGCAGAGTATGCTGGTCTGAAAGTAGCACGACGGCAAATGCAGGATGCTGC
  TGGCCGTCGACATTTTCATGCGTCTCAGTGCCCTAGGCCCACAAGTCCCGTGTCTACAGA
  CAGCAACATGAGTGCCGCCGTAATGCAGAAAACCAGACCAGCCAAGAAACTGAAACACCA
  GCCAGGACATCTGCGCAGAGAAACCTACACAGATGATCTTCCACCACCTCCTGTGCCGCC
  ACCTGCTATAAAGTCACCTACTGCCCAATCCAAGACACAGCTGGAAGTACGACCTGTAGT
  GGTGCCAAAACTCCCTTCTATGGATGCAAGAACAGACAGATCATCAGACAGAAAAGGAAG
  CAGTTACAAGGGGAGAGAAGTGTTGGATGGAAGACAGGTTGTTGACATGCGAACAAATCC
  AGGTGATCCCAGAGAAGCACAGGAACAGCAAAATGACGGGAAAGGACGTGGAAACAAGGC
  AGCAAAACGAGACCTTCCACCAGCAAAGACTCATCTCATCCAAGAGGATATTCTACCTTA
  TTGTAGACCTACTTTTCCAACATCAAATAATCCCAGAGATCCCAGTTCCTCAAGCTCAAT
  GTCATCAAGAGGATCAGGAAGCAGACAAAGAGAACAAGCAAATGTAGGTCGAAGAAATAT
  TGCAGAAATGCAGGTACTTGGAGGATATGAAAGAGGAGAAGATAATAATGAAGAATTAGA
  GGAAACTGAAAGCTGAAGACAACCAAGAGGCTTATGAGATCTAATGTGAAAATCATCACT
  CAAGATGCCTCCTGTCAGATGACACATGACGCCAGATAAAATGTTCAGTGCAATCAGAGT
  GTACAAATTGTCGTTTTTATTCCTCTTATTGGGATATCATTTTAAAAACTTTATTGGGTT
  TTTATTGTTGTTGTTTGATCCCTAACCCTACAAAGAGCCTTCCTATTCCCCTCGCTGTTG
  G
  
• Restriction Sites: Please inquire
• ACCN: NM_001145844
• Insert Size: 5300 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_001145844.1.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001145844.1, NP_001139316.1
• RefSeq Size: 7550 bp
• RefSeq ORF: 4821 bp
• Locus ID: 6091
• Cytogenetics: 3p12.3
• Protein Families: Druggable Genome
• Protein Pathways: Axon guidance
• Gene Summary: Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
  Transcript Variant: This variant (3) represents use of an alternate promoter and 5' UTR, uses a distinct translation start site, includes an alternate in-frame exon in the 5' coding region, and lacks an alternate in-frame exon in the central coding region, compared to variant 1. The resulting isoform (c) has a shorter and distinct N-terminus and differs at two internal regions, compared to isoform a. The 5' UTR of this variant contains a 55 aa uORF with a strong Kozak signal that may inhibit translation of this protein.