OGA (NM_001142434) Human Untagged Clone

CAT#: SC326262

MGEA5 (untagged)-Human meningioma expressed antigen 5 (hyaluronidase) (MGEA5), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: OGA
• Synonyms: MEA5; MGEA5; NCOAT
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC326262 sequence for NM_001142434 edited (data generated by NextGen Sequencing)
  
  
  ATGGTGCAGAAGGAGAGTCAAGCGACGTTGGAGGAGCGGGAGAGCGAGCTCAGCTCCAACCCTGCCGCCT
  CTGCGGGGGCATCGCTGGAGCCGCCGGCAGCTCCGGCACCCGGAGAAGACAACCCCGCCGGGGCTGGGGG
  AGCGGCGGTGGCCGGGGCTGCAGGAGGGGCTCGGCGGTTCCTCTGCGGTGTGGTGGAAGGATTTTATGGA
  AGACCTTGGGTTATGGAACAGAGAAAAGAACTCTTTAGAAGGCTCCAGAAATGGGAATTAAATACATACT
  TGTATGCCCCAAAAGATGACTACAAACATAGGATGTTTTGGCGAGAGATGTATTCAGTGGAGGAAGCTGA
  GCAACTTATGACTCTCATCTCTGCTGCACGAGAATATGAGATAGAGTTCATCTATGCGATCTCACCTGGA
  TTGGATATCACTTTTTCTAACCCCAAGGAAGTATCCACATTGAAACGTAAATTGGACCAGGTTTCTCAGT
  TTGGGTGCAGATCATTTGCTTTGCTTTTTGATGATATAGACCATAATATGTGTGCAGCAGACAAAGAGGT
  ATTCAGTTCTTTTGCTCATGCCCAAGTCTCCATCACAAATGAAATCTATCAGTACCTAGGAGAGCCAGAA
  ACTTTCCTCTTCTGTCCCACAGAATACTGTGGCACTTTCTGTTATCCAAATGTGTCTCAGTCTCCATATT
  TAAGGACTGTGGGTGAAAAGCTTCTACCTGGAATTGAAGTGCTTTGGACAGGTCCCAAAGTTGTTTCTAA
  AGAAATTCCAGTAGAGTCCATCGAAGAGGTTTCTAAGATTATTAAGAGAGCTCCAGTAATCTGGGATAAC
  ATTCATGCTAATGATTATGATCAGAAGAGACTGTTTCTGGGCCCGTACAAAGGAAGATCCACAGAACTCA
  TCCCACGGTTAAAAGGAGTCCTCACTAATCCAAATTGTGAATTTGAAGCCAACTACGTTGCTATCCACAC
  CCTTGCCACCTGGTACAAATCAAACATGAATGGAGTGAGAAAAGATGTAGTGATGAGTAGGCAAGTTGCA
  CACAGTGGAGCTAAAGCAAGTGTAGTTGATGGGACTCCTTTAGTTGCAGCACCCTCTTTAAATGCCACAA
  CCGTAGTAACAACAGTTTATCAGGAGCCCATTATGAGCCAGGGAGCAGCCTTGAGTGGTGAGCCTACTAC
  TCTGACCAAGGAAGAAGAAAAGAAACAGCCTGATGAAGAACCCATGGACATGGTGGTGGAAAAACAAGAA
  GAAACGGACCACAAGAATGACAATCAAATACTGAGTGAAATTGTTGAAGCGAAAATGGCAGAGGAATTGA
  AACCAATGGACACTGATAAAGAGAGCATAGCTGAATCAAAATCCCCAGAGATGTCCATGCAAGAAGATTG
  TATTAGTGACATTGCCCCCATGCAAACTGATGAACAGACAAACAAGGAGCAGTTTGTGCCAGGTCCAAAT
  GAAAAGCCTTTGTACACTGCGGAACCAGTGACCCTGGAGGATTTGCAGTTACTTGCTGATCTATTCTACC
  TTCCTTACGAGCATGGACCCAAAGGAGCACAGATGTTACGGGAATTTCAATGGCTTCGAGCAAATAGTAG
  TGTTGTCAGTGTCAATTGCAAAGGAAAAGACTCTAAAAAAATTGAAGAATGGCGGTCACGAGCAGCCAAG
  TTTGAAGAGATGTGTGGACTAGTGATGGGAATGTTCACTCGGCTCTCCAATTGTGCCAACAGGACAATTC
  TTTATGACATGTACTCCTATGTTTGGGATATCAAGAGTATAATGTCTATGGTGAAGTCTTTTGTACAGTG
  GTTAGGGTGTCGTAGTCATTCTTCAGCACAATTCTTAATTGGAGACCAAGAACCCTGGGCCTTTAGAGGT
  GGTCTAGCAGGAGAGTTCCAGCGTTTGCTGCCAATTGATGGGGCAAATGATCTCTTTTTTCAGCCACCTC
  CACTGACTCCTACCTCCAAAGTTTATACTATCAGACCTTATTTTCCTAAGGATGAGGCATCCGTGTACAA
  GATTTGCAGAGAAATGTATGACGATGGAGTGGGTTTACCCTTTCAAAGTCAGCCTGATCTTATTGGAGAC
  AAGTTAGTAGGAGGGCTGCTTTCCCTCAGCCTGGATTACTGCTTTGTCCTAGAAGATGAAGATGGCATAT
  GTGGTTATGCCTTGGGCACTGTAGATGTGACCCCCTTTATTAAAAAATGTAAAATTTCCTGGATCCCCTT
  CATGCAGGAGAAGTATACCAAGCCAAATGGTGACAAGGAACTCTCTGAGGCTGAGAAAATAATGTTGAGT
  TTCCATGAAGAACAGGAAGTACTGCCAGAAACTTTCCTTGCTAATTTCCCTTCTCTGATAAAGATGGACA
  TTCACAAAAAAGTAACTGACCCAAGTGTGGCCAAAAGCATGATGGCTTGCCTCCTGTCTTCACTGAAGGC
  TAATGGCTCCCGGGGAGCTTTCTGTGAAGTGAGACCAGATGATAAAAGAATTCTGGAATTTTACAGCAAG
  TTAGGATGTTTTGAAATTGCAAAAATGGAAGGATTTCCAAAGGATGTGGTTATACTTGGTCGGAGCCTGT
  GA
  
  Clone variation with respect to NM_001142434.1
  1645:a=>g
• Restriction Sites: Please inquire
• ACCN: NM_001142434
• Insert Size: 3100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001142434.1, NP_001135906.1
• RefSeq Size: 5061 bp
• RefSeq ORF: 2592 bp
• Locus ID: 10724
• UniProt ID: O60502
• Cytogenetics: 10q24.32
• Gene Summary: The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (Gao et al., 2001 [PubMed 11148210]).[supplied by OMIM, Mar 2008]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the coding region, compared to variant 1. This results in a shorter protein (isoform b), compared to isoform a.