RNF156 (MGRN1) (NM_001142291) Human Untagged Clone

CAT#: SC326035

MGRN1 (untagged)-Human mahogunin, ring finger 1 (MGRN1), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RNF156
• Synonyms: RNF156
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC326035 representing NM_001142291.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGGCTCCATTCTCAGCCGCCGCATCGCGGGGGTGGAGGACATCGACATCCAGGCGAACTCGGCCTAT
  CGCTACCCTCCGAAGTCCGGAAACTACTTTGCTTCGCACTTTTTCATGGGAGGAGAGAAATTCGACACC
  CCCCACCCTGAAGGTTACCTCTTTGGAGAGAACATGGATCTGAACTTCCTGGGCAGCCGCCCGGTCCAG
  TTTCCCTACGTCACTCCTGCCCCCCACGAGCCCGTGAAGACGCTGCGGAGCCTGGTGAACATCCGCAAA
  GACTCCCTGCGGCTGGTGAGGTACAAAGACGATGCCGACAGCCCCACCGAGGACGGCGACAAGCCCCGG
  GTGCTCTACAGCCTGGAGTTCACCTTCGACGCCGATGCCCGCGTGGCCATCACCATCTACTGCCAGGCA
  TCGGAGGAGTTCCTGAACGGCAGGGCAGTATACAGCCCCAAGAGCCCCTCGCTACAGTCCGAGACCGTC
  CACTACAAGAGAGGGGTGAGCCAGCAGTTCTCCCTGCCCTCCTTCAAGATTGACTTCTCGGAATGGAAG
  GATGACGAGCTGAACTTTGACCTGGACCGGGGCGTGTTTCCAGTAGTCATCCAGGCTGTGGTGGACGAA
  GGAGATGTGGTGGAAGTGACTGGCCACGCCCACGTGCTCTTGGCTGCCTTTGAAAAGCACATGGACGGC
  AGCTTCTCTGTGAAGCCTTTAAAGCAGAAGCAAATTGTGGACCGGGTCAGCTACCTCCTGCAGGAGATC
  TATGGCATTGAGAACAAGAACAACCAGGAGACCAAGCCCTCGGACGACGAGAACAGCGACAACAGCAAC
  GAGTGTGTGGTGTGCCTGTCCGACCTGCGGGACACGCTGATCCTGCCCTGCCGCCACCTGTGCCTCTGT
  ACCTCCTGCGCCGACACGCTGCGCTACCAGGCCAACAACTGCCCCATCTGCCGGCTGCCTTTCCGGGCC
  CTCCTGCAGATCCGGGCGGTGCGGAAGAAGCCAGGAGCCCTGTCCCCCGTGTCCTTCAGCCCCGTCCTG
  GCCCAGAGCCTGGAGCATGATGAGCACTCTAACTCTGACAGCGTCCCACCTGGCTACGAGCCCATCTCG
  CTGCTCGAGGCGCTCAACGGCCTCCGGGCTGTCTCCCCGGCCATCCCCTCGGCCCCTCTTTATGAAGAA
  ATCACCTATTCAGGCATCTCGGACGGCCTGTCCCAGGCCAGCTGTCCCCTCGCGGCTATCGACCACATC
  CTGGACAGCAGCCGCCAGAAGGGCAGGCCGCAGAGCAAGGCCCCCGACAGCACCCTACGGTCCCCGTCT
  TCCCCCATCCACGAAGAGGATGAGGAGAAGCTCTCCGAGGACGTGGACGCCCCTCCCCCACTGGGTGGC
  GCAGAGCTGGCCCTGCGGGAAAGCAGCTCCCCTGAGAGTTTCATAACAGAAGAGGTTGATGAGTCGTCG
  TCACCACAGCAAGGGACCCGAGCAGCTTCCATTGAGAATGTCCTGCAGGACAGCAGCCCCGAGCACTGT
  GGCCGAGGCCCACCTGCTGACATCTACCTGCCAGCCCTGGGGCCCGACTCCTGCTCTGTTGGTATAGAC
  GAGTAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001142291
• Insert Size: 1593 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001142291.2
• RefSeq Size: 6405 bp
• RefSeq ORF: 1593 bp
• Locus ID: 23295
• UniProt ID: O60291
• Cytogenetics: 16p13.3
• Protein Pathways: Ubiquitin mediated proteolysis
• MW: 58.3 kDa
• Gene Summary: Mahogunin (MGRN1) is a C3HC4 RING-containing protein with E3 ubiquitin ligase activity in vitro.[supplied by OMIM, Apr 2004]
  Transcript Variant: This variant (4) lacks an alternate in-frame exon and uses an alternate splice junction at the 5' end of the last exon compared to variant 1. The resulting isoform (4) is shorter and has a distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.