ZNF286 (ZNF286A) (NM_001130842) Human Untagged Clone

CAT#: SC326021

ZNF286A (untagged)-Human zinc finger protein 286A (ZNF286A), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ZNF286
• Synonyms: ZNF286
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC326021 representing NM_001130842.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGAGACAGATTTGGCTGAAATGCCTGAGAAAGGAGCTCTGTCTTCCCAGGATTCTCCCCATTTCCAA
  GAGAAGAGCACAGAAGAGGGAGAAGTGGCTGCTCTGCGCCTCACGGCCAGATCCCAGGAAACAGTGACA
  TTCAAGGATGTGGCCATGGACTTTACACCAGAGGAGTGGGGGAAGCTGGATCCTGCACAAAGGGATGTG
  ATGCTGGAGAACTATAGGAACCTAGTCTCACTTTGGCTTCCAGTTTCCAAACCTGAGAGCTACAACTTG
  GAGAATGGAAAAGAACCATTGAAGCTTGAGAGAAAAGCCCCCAAAAGCAGCTATTCAGACATGGAGACT
  AGACCACAGAGCAAGGATTCAACTTCAGTGCAAGATTTTTCCAAAGCAGAATCATGCAAAGTTGCAATA
  ATAGACAGACTGACACGGAATAGTGTCTATGACTCTAACTTGGAAGCAGCCCTTGAATGTGAAAATTGG
  TTAGAGAATCAGCAAGGAAATCAGGAGAGACATTTGAGAGAAATGTTCACTCACATGAATTCACTCTCT
  GAGGAAACAGACCATAAGCATGATGTATACTGGAAAAGCTTCAATCAGAAATCTGTCCTTATCACTGAA
  GACAGAGTTCCCAAAGGATCTTATGCCTTCCATACACTTGAAAAAAGCTTGAAACAAAAATCAAACTTA
  ATGAAAAAGCAGAGAACTTATAAAGAGAAAAAACCTCATAAATGTAATGATTGTGGTGAACTCTTCACC
  TACCATTCAGTGCTTATTCGACACCAGAGAGTCCATACTGGAGAGAAACCCTATACCTGCAATGAATGT
  GGGAAATCTTTTAGCCACAGAGCTAATTTAACTAAACACCAGAGAACTCATACTAGAATTCTCTTTGAA
  TGCAGTGAATGCAAGAAAACCTTCACAGAAAGCTCATCCCTTGCAACACATCAGAGAATTCACGTTGGA
  GAGAGACCTTATGAATGCAATGAATGTGGGAAAGGTTTTAATCGAAGTACACATCTTGTGCAGCATCAG
  TTGATTCATACTGGAGTGAAGCCTTATGAATGTAATGAATGTGATAAAGCTTTTATTCATTCATCAGCA
  CTCATTAAACATCAAAGAACTCATACTGGAGAGAAACCTTATAAATGTCAAGAATGTGGGAAAGCCTTT
  AGCCATTGCTCATCCCTAACTAAGCATCAGAGAGTTCATACTGGAGAAAAGCCATATGAATGCAGTGAA
  TGTGGAAAAACTTTTAGTCAGAGCACACATCTTGTTCAACATCAGAGAATTCACACTGGAGAGAAACCC
  TATGAGTGTAATGAATGTGGGAAAACCTTCAGCCGGAGCTCCAATTTTGCTAAACATCAAAGAATTCAT
  ATTGGAAAGAAACCGTACAAATGTAGCGAGTGTGGAAAAGCCTTCATTCATTCATCAGCTCTCATTCAA
  CATCAGAGAACTCATACCGGAGAGAAACCCTTTAGATGTAATGAGTGTGGGAAAAGCTTTAAGTGCAGT
  TCATCTCTCATCAGACATCAAAGAGTTCACACTGAAGAGCAACCCTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001130842
• Insert Size: 1566 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001130842.1
• RefSeq Size: 5472 bp
• RefSeq ORF: 1566 bp
• Locus ID: 57335
• UniProt ID: Q9HBT8
• Cytogenetics: 17p12
• Protein Families: Transcription Factors
• MW: 60.2 kDa
• Gene Summary: May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at a downstream start codon, compared to variant 3. Variants 1 and 2 encode the same isoform (2), which has a shorter N-terminus, compared to isoform 1.