Tropomodulin 2 (TMOD2) (NM_001142885) Human Untagged Clone

CAT#: SC325761

TMOD2 (untagged)-Human tropomodulin 2 (neuronal) (TMOD2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TMOD2
• Synonyms: N-TMOD; NTMOD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC325761 representing NM_001142885.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCACTCCCCTTTCAAAAAGAGCTGGAGAAATACAAGAACATTGATGAAGATGAGCTTCTTGGCAAA
  CTCTCAGAAGAGGAACTGAAACAGTTGGAAAATGTTCTAGATGACCTAGATCCTGAGAGTGCCATGCTG
  CCAGCTGGATTTCGACAGAAAGACCAGACACAGAAGGCAGCCACCGGCCCCTTTGACCGCGAGCACCTC
  CTCATGTACCTGGAGAAGGAGGCTTTGGAACAGAAAGACAGAGAGGACTTTGTGCCCTTCACTGGAGAA
  AAGAAAGGGAGAGTCTTTATCCCTAAAGAAAAGCCTATAGAAACTCGTAAAGAAGAAAAAGTGACCCTT
  GACCCAGAACTGGAAGAAGCTTTGGCCAGTGCCTCTGACACCGAACTCTATGATCTTGCAGCTGTCCTT
  GGAGTACACAATTTGCTCAACAATCCAAAGTTCGATGAAGAAACAGCCAACAATAAAGGTGGCAAAGGA
  CCTGTCAGAAATGTTGTCAAAGGTGAAAAAGTAAAGCCAGTATTTGAGGAACCACCAAATCCCACAAAT
  GTGGAAATAAGCCTGCAGCAGATGAAAGCCAATGATCCTAGCTTGCAAGAAGTCAACCTCAACAACATT
  AAGGCTTTTGCAGACATGCTGAAAGTAAACAAGACCTTGACAAGTCTAAACATAGAATCCAATTTTATC
  ACTGGAACTGGGATCCTGGCCCTGGTAGAGGCACTGAAAGAAAATGACACCTTGACAGAAATCAAGATT
  GACAACCAGAGGCAGCAGTTGGGAACAGCTGTAGAGATGGAAATTGCCCAGATGCTGGAGGAGAATTCA
  AGGATCCTCAAGTTTGGATACCAGTTTACCAAGCAAGGGCCACGAACAAGGGTGGCAGCTGCCATCACA
  AAGAATAATGACCTGGTTCGTAAGAAGAGAGTTGAAGCAGACCGAAGGTAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001142885
• Insert Size: 948 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001142885.1
• RefSeq Size: 9078 bp
• RefSeq ORF: 948 bp
• Locus ID: 29767
• UniProt ID: Q9NZR1
• Cytogenetics: 15q21.2
• MW: 35.6 kDa
• Gene Summary: This gene encodes a neuronal-specific member of the tropomodulin family of actin-regulatory proteins. The encoded protein caps the pointed end of actin filaments preventing both elongation and depolymerization. The capping activity of this protein is dependent on its association with tropomyosin. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Dec 2008]
  Transcript Variant: This variant (2) lacks an exon in the coding region compared to variant 1. The encoded protein (isoform b) is shorter compared to isoform a.