TLK1 (NM_001136554) Human Untagged Clone

CAT#: SC325302

TLK1 (untagged)-Human tousled-like kinase 1 (TLK1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TLK1
• Synonyms: PKU-beta
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001136554, the custom clone sequence may differ by one or more nucleotides
  ATGGATGAGCTTCATAGTCTGGATCCAAGAAGGCAAGAGTTATTGGAAGCTAGATTTACT
  GGAGTTGCAAGTGGGAGCACTGGAAGTACGGGCAGTTGCAGTGTTGGAGCTAAAGCCTCA
  ACAAATAACGAAAGCTCTAATCACAGTTTTGGAAGCTTGGGATCTTTAAGTGACAAAGAA
  TCAGAGACACCGGAGAAGAAACAATCGGAATCATCCAGGGGAAGAAAGAGAAAAGCAGAA
  AACCAGAATGAAAGTAGTCAGGGAAAAAGTATTGGGGGACGTGGCCACAAAATTAGCGAC
  TATTTTGAATACCAGGGTGGAAATGGCTCAAGTCCAGTAAGAGGCATACCTCCTGCAATC
  CGTTCTCCTCAAAATTCACATTCACATTCCACTCCTTCCTCATCTGTTCGACCGAATAGC
  CCTTCTCCTACTGCATTAGCATTTGGGGACCACCCTATTGTACAACCAAAGCAATTATCC
  TTTAAAATTATTCAGACTGATCTCACAATGCTGAAATTAGCAGCATTAGAAAGTAATAAA
  ATCCAGGACCTGGAAAAGAAGGAGGGACGTATAGATGATTTGCTCAGGGCTAACTGTGAT
  CTCAGACGGCAAATAGATGAACAACAAAAATTACTTGAAAAATACAAAGAACGATTAAAT
  AAGTGCATATCAATGAGCAAGAAACTTCTTATTGAAAAGAGTACACAAGAAAAGCTGTCA
  AGCAGAGAGAAGAGTATGCAAGATCGATTACGCCTCGGGCACTTTACAACAGTTAGACAT
  GGCGCTTCATTTACTGAACAATGGACAGATGGTTTTGCATTTCAGAATCTTGTGAAGCAA
  CAAGAATGGGTGAATCAGCAAAGGGAAGATATTGAAAGGCAAAGGAAACTTCTAGCCAAA
  CGCAAACCTCCCACAGCTAATAATTCTCAGGCACCCTCTACCAATTCTGAACCAAAACAA
  AGGAAAAACAAAGCAGTCAATGGAGCAGAGAATGATCCCTTTGTTAGACCAAATTTACCA
  CAACTGTTGACTTTGGCAGAATATCATGAACAGGAAGAAATTTTCAAACTTAGACTAGGA
  CATCTCAAAAAGGAAGAGGCAGAAATCCAGGCAGAACTTGAACGTTTGGAAAGAGTCAGA
  AATCTTCACATACGTGAGCTGAAAAGAATAAACAATGAAGATAATTCACAGTTCAAAGAT
  CACCCAACATTAAATGAAAGATATTTATTACTTCATCTGCTTGGTAGAGGTGGCTTTAGT
  GAAGTGTATAAGGCTTTTGACCTTTATGAACAAAGATATGCTGCTGTGAAGATACATCAG
  CTTAATAAAAGCTGGAGAGATGAGAAGAAAGAAAACTACCACAAACATGCCTGCAGAGAG
  TATAGAATACACAAAGAACTGGATCACCCCAGAATAGTTAAACTCTATGATTATTTCTCC
  TTGGATACAGATACGTTTTGTACAGTGTTAGAATACTGTGAAGGCAATGACTTGGATTTC
  TATCTGAAGCAACACAAGTTAATGTCAGAGAAAGAAGCTCGGTCTATTGTAATGCAGATT
  GTAAATGCACTAAGATATCTCAATGAGATCAAACCCCCTATTATACATTATGATCTTAAG
  CCAGGAAACATCCTACTGGTAGATGGAACAGCATGTGGTGAAATCAAAATCACTGATTTT
  GGTCTGTCCAAGATTATGGATGATGATAGCTATGGTGTAGATGGAATGGATCTAACTTCC
  CAGGGGGCAGGCACTTACTGGTATTTACCTCCTGAGTGTTTTGTAGTTGGAAAAGAGCCA
  CCAAAGATTTCCAACAAGGTTGATGTATGGTCGGTTGGAGTCATCTTCTTTCAGTGTCTT
  TATGGTAGAAAGCCATTTGGTCACAATCAATCTCAACAAGACATTCTTCAAGAAAATACA
  ATATTAAAAGCCACAGAAGTCCAGTTCCCTGTAAAACCGGTTGTAAGCAGTGAAGCCAAG
  GCATTTATAAGACGCTGTTTGGCATATCGAAAAGAAGATCGATTTGATGTGCACCAGCTG
  GCAAATGACCCATACCTTCTCCCACACATGAGAAGATCAAATTCTTCAGGAAACCTACAC
  ATGGCTGGGCTGACAGCATCCCCTACACCCCCTTCTTCAAGCATAATTACTTAC
  
• Restriction Sites: Please inquire
• ACCN: NM_001136554
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001136554.1, NP_001130026.1
• RefSeq Size: 5310 bp
• RefSeq ORF: 2157 bp
• Locus ID: 9874
• UniProt ID: Q9UKI8
• Cytogenetics: 2q31.1
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: The protein encoded by this gene is a serine/threonine kinase that may be involved in the regulation of chromatin assembly. The encoded protein is only active when it is phosphorylated, and this phosphorylation is cell cycle-dependent, with the maximal activity of this protein coming during S phase. The catalytic activity of this protein is diminished by DNA damage and by blockage of DNA replication. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
  Transcript Variant: This variant (2) differs in the 5' UTR and coding region, and uses a downstream start codon, compared to variant 1, resulting in a shorter protein (isoform 2).