DCTN4 (NM_001135644) Human Untagged Clone

CAT#: SC325054

DCTN4 (untagged)-Human dynactin 4 (p62) (DCTN4), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DCTN4
• Synonyms: DYN4; P62
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001135644, the custom clone sequence may differ by one or more nucleotides
  ATGCCATCGGCTGAAGCCAAACTAAAAAAGAATAGATGTGCCAATTGTTTTGACTGTCCT
  GGCTGCATGCACACCCTCTCTACTCGGGCCACGAGCATCTCCACACAGCTTCCAGATGAC
  CCAGCCAAGACCACCATGAAGAAAGCCTATTACCTGGCATGTGGATTTTGTCGCTGGACG
  TCTAGAGATGTGGGCATGGCAGACAAATCTGTAGCTAGTGGCGGTTGGCAGGAACCTGAA
  AATCCTCACACACAACGGATGAACAAATTGATTGAATATTACCAGCAGCTTGCTCAGAAA
  GAGAAGGTTGAGCGAGATCGCAAGAAACTGGCACGACGTAGAAACTATATGCCTCTGGCT
  TTTTCGGACAAATATGGTCTTGGAACCAGGCTTCAGCGACCACGAGCTGGTGCATCCATC
  AGTACCCTTGCCGGACTTTCCCTTAAAGAAGGAGAGGATCAGAAAGAGATAAAGATTGAG
  CCAGCTCAGGCTGTGGATGAAGTGGAACCTCTACCTGAAGACTATTATACAAGACCAGTA
  AATTTAACAGAGGTAACAACCCTTCAGCAGCGTCTGTTACAGCCTGACTTCCAGCCAGTC
  TGTGCTTCACAGCTCTATCCTCGCCACAAACATCTTCTGATCAAACGGTCCCTGCGCTGC
  CGTAAATGTGAACATAATTTGAGCAAGCCAGAATTTAACCCAACGTCAATCAAATTCAAA
  ATCCAGCTGGTCGCTGTCAATTATATTCCAGAAGTGAGAATCATGTCAATTCCCAACCTT
  CGCTACATGAAGGAGAGCCAGGTCCTCCTGACTCTTACAAATCCAGTTGAGAACCTCACC
  CATGTGACTCTCTTCGAGTGTGAGGAGGGGGACCCTGATGATATCAACAGCACTGCTAAG
  GTGGTGGTGCCTCCCAAAGAGCTCGTTTTAGCTGGCAAGGATGCAGCAGCAGAGTACGAT
  GAGTTGGCAGAACCTCAAGACTTTCAGGACGATCCTGACATTATAGCCTTCAGAAAGGCC
  AACAAAGTGGGTATTTTCATCAAAGTTACACCACAGCGTGAGGAGGGTGAAGTGACCGTG
  TGCTTCAAGATGAAGCATGATTTTAAAAACCTGGCAGCCCCCATTCGCCCCATTGAAGAA
  AGTGACCAGGGAACAGAAGTCATCTGGCTCACCCAGCATGTGGAACTTAGCTTGGGCCCA
  CTTCTTCCT
  
• Restriction Sites: Please inquire
• ACCN: NM_001135644
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001135644.1, NP_001129116.1
• RefSeq Size: 4105 bp
• RefSeq ORF: 1212 bp
• Locus ID: 51164
• UniProt ID: Q9UJW0
• Cytogenetics: 5q33.1
• Protein Pathways: Huntington's disease
• Gene Summary: Could have a dual role in dynein targeting and in ACTR1A/Arp1 subunit of dynactin pointed-end capping. Could be involved in ACTR1A pointed-end binding and in additional roles in linking dynein and dynactin to the cortical cytoskeleton.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence and lacks an alternate in-frame exon compared to variant 1. The resulting isoform (c) is shorter at the N-terminus and lacks a 7 aa internal segment compared to isoform a.